The aim of this study was to determine the prevalence of M. hyopneumoniae infection in suckling pigs. Nasal swabs were collected from 300 suckling pigs originating from five farrow-to-finishing farms. One farm had a c...The aim of this study was to determine the prevalence of M. hyopneumoniae infection in suckling pigs. Nasal swabs were collected from 300 suckling pigs originating from five farrow-to-finishing farms. One farm had a confirmed PRDC problem (farm A) and four other farms previously had a PRDC problem (farms B, C, D and E). Thirty (30) lactating sows in parity 1, 2 and 3 were selected from each farm (Ten sows per parity). Two piglets from each sow were randomly sampled for nasal swab at 3 weeks of age. The samples were analyzed by the nested PCR technique. Forty five per cent (27/60) of nasal swabs from farm A were found positive. On the other hand, a total of 2.08 per cent were found positive (5/240) from farm B, C, D and E. The tendency of piglet infection per sow by parity showed that first parity had more prevalence than the second and the third parity (60%, 55%, 20%), respectively. We have found a correction between M. hyopneumoniae early infection in suckling pigs and a confirmed PRDC problem (farm A) as oppose to farms that did not have a PRDC problem. The strategies to prevent M. hyopneumoniae early infection are to maintain good lactation, antibiotic prevention program and early M. hyopneumoniae展开更多
Multidrug resistance(MDR)problems become critical concerns in animal production through food chains and the environment.Thus,the cross-sectional study of E.coli in swine feces,drinking water,and wastewater collected o...Multidrug resistance(MDR)problems become critical concerns in animal production through food chains and the environment.Thus,the cross-sectional study of E.coli in swine feces,drinking water,and wastewater collected on four medium and four large swine farms in Thailand was studied.The orders of antibiotics used were AMX-COL-GM-TIL and AMX-COL-GM-CER on medium-sized and large swine farms,respectively.Hemolytic E.coli(HEC)was more frequently isolated from all samples than Non-hemolytic E.coli(NHEC).The medium-sized farms were resistant to AMP,AMX,TE,and C,while large farms were resistant to AMP,AMX,TE,and PIP.Only two sensitive antibiotics,namely AK and TE,were available on both farms,whereas,banned NT was still detected in the sludge.The MDR of E.coli isolates was 95.7%and 87.7%on medium-sized and large swine farms,while the highest resistance found was 15 drugs used for NHEC on medium-sized swine farms.The PFGE molecular typing revealed the largest cluster containing five isolations,correlated with drinking water,sow,and fattening isolates;however,the phenotypic and genotypic were not in a similar pattern.The most seriously MDR related to HEC was found in the piglets,effluent,and sludge.Thus,the AMR and MDR of E.coli existence in medium-sized and large swine farms in this study.MDR of E.coli was substantially found in the environmental,including effluent and sludge.Therefore,it inevitably operates the long-term monitoring of AMR,especially targeted piglets and its environment as the main focus of critical antibiotics use in swine farms.展开更多
文摘The aim of this study was to determine the prevalence of M. hyopneumoniae infection in suckling pigs. Nasal swabs were collected from 300 suckling pigs originating from five farrow-to-finishing farms. One farm had a confirmed PRDC problem (farm A) and four other farms previously had a PRDC problem (farms B, C, D and E). Thirty (30) lactating sows in parity 1, 2 and 3 were selected from each farm (Ten sows per parity). Two piglets from each sow were randomly sampled for nasal swab at 3 weeks of age. The samples were analyzed by the nested PCR technique. Forty five per cent (27/60) of nasal swabs from farm A were found positive. On the other hand, a total of 2.08 per cent were found positive (5/240) from farm B, C, D and E. The tendency of piglet infection per sow by parity showed that first parity had more prevalence than the second and the third parity (60%, 55%, 20%), respectively. We have found a correction between M. hyopneumoniae early infection in suckling pigs and a confirmed PRDC problem (farm A) as oppose to farms that did not have a PRDC problem. The strategies to prevent M. hyopneumoniae early infection are to maintain good lactation, antibiotic prevention program and early M. hyopneumoniae
文摘Multidrug resistance(MDR)problems become critical concerns in animal production through food chains and the environment.Thus,the cross-sectional study of E.coli in swine feces,drinking water,and wastewater collected on four medium and four large swine farms in Thailand was studied.The orders of antibiotics used were AMX-COL-GM-TIL and AMX-COL-GM-CER on medium-sized and large swine farms,respectively.Hemolytic E.coli(HEC)was more frequently isolated from all samples than Non-hemolytic E.coli(NHEC).The medium-sized farms were resistant to AMP,AMX,TE,and C,while large farms were resistant to AMP,AMX,TE,and PIP.Only two sensitive antibiotics,namely AK and TE,were available on both farms,whereas,banned NT was still detected in the sludge.The MDR of E.coli isolates was 95.7%and 87.7%on medium-sized and large swine farms,while the highest resistance found was 15 drugs used for NHEC on medium-sized swine farms.The PFGE molecular typing revealed the largest cluster containing five isolations,correlated with drinking water,sow,and fattening isolates;however,the phenotypic and genotypic were not in a similar pattern.The most seriously MDR related to HEC was found in the piglets,effluent,and sludge.Thus,the AMR and MDR of E.coli existence in medium-sized and large swine farms in this study.MDR of E.coli was substantially found in the environmental,including effluent and sludge.Therefore,it inevitably operates the long-term monitoring of AMR,especially targeted piglets and its environment as the main focus of critical antibiotics use in swine farms.
