Phoenix dactylifera L.seed protein hydrolysates as a potential source of peptides with antidiabetic and anti-hypercholesterolemic properties:An in vitro study

This study aimed to explore the effect of enzymatic hydrolysis conditions(enzyme types and hydrolysis time)of date seed protein hydrolysates(DSPH)on in-vitro inhibition of molecular markers related with diabetic and hypercholesteremia.The DSPH was prepared using alcalase,bromelain,papain,and protease at different hydrolysis time(HT)(2,4 and 6 h).Higher degree of hydrolysis was observed for papain at 6 h HT.The results showed that alcalase and bromelain generated DSPHs at 6 h HT greatly improved the pancreatic lipase inhibition activity while the cholesterol esterase inhibition activity was greatly enhanced with alcalase and protease at 6 h HT and papain generated DSPHs at 4 h HT.Bromelain generated DSPH displayed the highest inhibition of dipeptidyl peptidase-IV(DPP-IV)and amylase at 6 and 2 h hydrolysis time,respectively.All the enzymes at 2 h HT except that of papain showed the highestα-glucosidase inhibitory activities.These results revealed that the DSPH displayed enhanced inhibitory activities towards molecular markers related with diabetes and obesity and thus have promising potential to be used as health-promoting ingredients in the fabrication of functional foods.