The Na+ - K+ ATPase is an enzyme responsible for the active transport of Na+ and K+ in most eukaryotic cells. The aim of the present study was to determine the effect of Tachykinin neuropeptide, Neurokinin B (NKB) and...The Na+ - K+ ATPase is an enzyme responsible for the active transport of Na+ and K+ in most eukaryotic cells. The aim of the present study was to determine the effect of Tachykinin neuropeptide, Neurokinin B (NKB) and Amyloid beta fragment Aβ (25 - 35) on 17β estradiol (E2) treated aging female rat brain synaptosomes of different age groups, by assaying Na+ - K+ ATPase enzyme activity. An in vitro incubation of isolated synaptosomes with Aβ (25 - 35) showed toxic effects while NKB showed stimulating effect on the Na+ - K+ ATPase activity, and the combined NKB + Aβ (25 - 35) incubations showed a partial effect as compared to the Aβ (25 - 35) alone. To understand whether E2 affects the expression of Na+ - K+ ATPase molecules, we examined the expression of Na+ - K+ ATPase subunit α1 and β2 in E2 treated aging female rat brain synaptosomes. The enzyme was quantified by SDS PAGE in control and E2 treated rat brain. We observed that the expression of α1 and β2 Na+ - K+ ATPase molecules increased and reversed to a normal level in E2 treated synaptosomes. These results confirmed that E2 increased turnover of Na+ - K+ ATPase molecules in aging rat brain. The present findings also suggest a possible role of NKB with E2 in the age related changes in the brain.展开更多
BACKGROUND Nursing officers are an integral component of any medical team.They participate in taking care of basic airway management and assist in advanced airway management,specifically amidst the current coronavirus...BACKGROUND Nursing officers are an integral component of any medical team.They participate in taking care of basic airway management and assist in advanced airway management,specifically amidst the current coronavirus disease 2019(COVID-19)pandemic.AIM To assess the efficacy of a standardized web-based training module for nurses in preparedness to fight against COVID-19.METHODS The training was held in three sessions of 1 h each,consisting of live audio-visual lectures,case scenarios,and skill demonstrations.The sequence of airway equipment,drug preparation,airway examination,and plans of airway management was demonstrated through mannequin-based video-clips.RESULTS Pre-and post-test scores as well as objective structured clinical examination scores were analyzed using Student’s t-test and the Likert scale was used for feedback assessment.It was found that the mean score out of the total score of 20 was 8.47±4.2 in the pre-test,while in the post-test it was 17.4±1.8(P value<0.001).The participants also felt self-reliant in executing the roles of airway assistant(63.3%)and drug assistant(74.3%).Fear of self-infection with COVID-19 was also high,as 66%of participants feared working with the patient’s airway.CONCLUSION Amidst this COVID-19 emergency,when the health care systems are being persistently challenged,training of nursing staff in the safe conduct of airway management can ensure delivery of life-saving treatment.展开更多
文摘The Na+ - K+ ATPase is an enzyme responsible for the active transport of Na+ and K+ in most eukaryotic cells. The aim of the present study was to determine the effect of Tachykinin neuropeptide, Neurokinin B (NKB) and Amyloid beta fragment Aβ (25 - 35) on 17β estradiol (E2) treated aging female rat brain synaptosomes of different age groups, by assaying Na+ - K+ ATPase enzyme activity. An in vitro incubation of isolated synaptosomes with Aβ (25 - 35) showed toxic effects while NKB showed stimulating effect on the Na+ - K+ ATPase activity, and the combined NKB + Aβ (25 - 35) incubations showed a partial effect as compared to the Aβ (25 - 35) alone. To understand whether E2 affects the expression of Na+ - K+ ATPase molecules, we examined the expression of Na+ - K+ ATPase subunit α1 and β2 in E2 treated aging female rat brain synaptosomes. The enzyme was quantified by SDS PAGE in control and E2 treated rat brain. We observed that the expression of α1 and β2 Na+ - K+ ATPase molecules increased and reversed to a normal level in E2 treated synaptosomes. These results confirmed that E2 increased turnover of Na+ - K+ ATPase molecules in aging rat brain. The present findings also suggest a possible role of NKB with E2 in the age related changes in the brain.
文摘BACKGROUND Nursing officers are an integral component of any medical team.They participate in taking care of basic airway management and assist in advanced airway management,specifically amidst the current coronavirus disease 2019(COVID-19)pandemic.AIM To assess the efficacy of a standardized web-based training module for nurses in preparedness to fight against COVID-19.METHODS The training was held in three sessions of 1 h each,consisting of live audio-visual lectures,case scenarios,and skill demonstrations.The sequence of airway equipment,drug preparation,airway examination,and plans of airway management was demonstrated through mannequin-based video-clips.RESULTS Pre-and post-test scores as well as objective structured clinical examination scores were analyzed using Student’s t-test and the Likert scale was used for feedback assessment.It was found that the mean score out of the total score of 20 was 8.47±4.2 in the pre-test,while in the post-test it was 17.4±1.8(P value<0.001).The participants also felt self-reliant in executing the roles of airway assistant(63.3%)and drug assistant(74.3%).Fear of self-infection with COVID-19 was also high,as 66%of participants feared working with the patient’s airway.CONCLUSION Amidst this COVID-19 emergency,when the health care systems are being persistently challenged,training of nursing staff in the safe conduct of airway management can ensure delivery of life-saving treatment.
