目的:通过噬菌体展示技术制备抗人IgE纳米抗体,将其用于磁微粒化学发光平台建立一种猫皮屑特异性IgE抗体超敏检测方法。方法:在WHO/IUIS过敏原数据库中检索猫致敏蛋白信息,合成序列后在原核表达系统中表达和纯化猫皮屑致敏蛋白Fel d 1...目的:通过噬菌体展示技术制备抗人IgE纳米抗体,将其用于磁微粒化学发光平台建立一种猫皮屑特异性IgE抗体超敏检测方法。方法:在WHO/IUIS过敏原数据库中检索猫致敏蛋白信息,合成序列后在原核表达系统中表达和纯化猫皮屑致敏蛋白Fel d 1。使用人源IgE免疫新疆单峰驼提取淋巴细胞中的RNA建立噬菌体展示纳米抗体库,分析库容、多样性和插入率,通过筛选和鉴定获得抗人IgE纳米抗体。使用重组过敏原偶联磁微粒和吖啶酯标记纳米抗体,建立磁微粒化学法猫皮屑特异性IgE抗体检测方法。结果:噬菌体展示库的库容为1.88×108 cfu/ml,插入率为93.6%,纳米抗体纯度>95%。检测方法的线性范围为0.1~100 U/ml,与ImmunoCAP检测系统临床对比有较好的一致性。结论:成功建立了基于纳米抗体的猫皮屑特异性IgE抗体超敏检测方法,为猫过敏性疾病辅助诊断提供了技术基础。展开更多
Compressive tests of [0]t2 and [90]t2 unidirectional laminates and [45/0/-45/90] 2s quasi-isotropic laminates are accomplished in both room-temperature and dry （RTD） and hygrothermal environment. And simulation stud...Compressive tests of [0]t2 and [90]t2 unidirectional laminates and [45/0/-45/90] 2s quasi-isotropic laminates are accomplished in both room-temperature and dry （RTD） and hygrothermal environment. And simulation studies on the compressive strength of Z-pinned laminates of [0112 and [45/0/-45/90] 2s are conducted by using finite element analysis （FEA）. A microstructural unit cell for FEA is created to simulate a representative laminates unit with one pin. Within the unit cell, the first directions of the elements＇ material coordinate systems are changed to simulate the fibres＇ deflecting around the pin. The hygrothermal effect is simulated by the material properties＇ adjustments which are determined by the compressive tests of non-pined laminates. The experimental results indicate that the percentage of reduction in the compressive modulus of Z-pinned laminates caused by Z-pin becomes smaller with the percentage of 0° fibres decreasing in the laminates; the compressive strength of quasi-isotropic laminates reduces and the percentage of the reduction in the compressive strength declines with Z-pin volume content increasing, and the moisture absorption ratio of the Z-pinned specimens is greater than that of the non-pinned specimens, because the cracks around Z-pin increase the moisture absorption. In addition, the simulations show that the deflection of fibres around Z-pin is the main factor for the reduction in the compressive strength of Z-pinned unidirectional laminates, the dilution of fibre volume content caused by resin-rich pocket is the principal factor for the decline in the compressive strength of Z-pinned quasi-istropic laminates, and the compressive strength of Z-pinned specimens in hygrothermal environment reduces as the result of superimposition of some factors, including the changes in material properties caused by hygrothermal environment, the deflection of fibres and the resin-rich pocket caused by Z-pin.展开更多
文摘目的:通过噬菌体展示技术制备抗人IgE纳米抗体,将其用于磁微粒化学发光平台建立一种猫皮屑特异性IgE抗体超敏检测方法。方法:在WHO/IUIS过敏原数据库中检索猫致敏蛋白信息,合成序列后在原核表达系统中表达和纯化猫皮屑致敏蛋白Fel d 1。使用人源IgE免疫新疆单峰驼提取淋巴细胞中的RNA建立噬菌体展示纳米抗体库,分析库容、多样性和插入率,通过筛选和鉴定获得抗人IgE纳米抗体。使用重组过敏原偶联磁微粒和吖啶酯标记纳米抗体,建立磁微粒化学法猫皮屑特异性IgE抗体检测方法。结果:噬菌体展示库的库容为1.88×108 cfu/ml,插入率为93.6%,纳米抗体纯度>95%。检测方法的线性范围为0.1~100 U/ml,与ImmunoCAP检测系统临床对比有较好的一致性。结论:成功建立了基于纳米抗体的猫皮屑特异性IgE抗体超敏检测方法,为猫过敏性疾病辅助诊断提供了技术基础。
基金National Basic Research Program of China(2011CB606105)
文摘Compressive tests of [0]t2 and [90]t2 unidirectional laminates and [45/0/-45/90] 2s quasi-isotropic laminates are accomplished in both room-temperature and dry （RTD） and hygrothermal environment. And simulation studies on the compressive strength of Z-pinned laminates of [0112 and [45/0/-45/90] 2s are conducted by using finite element analysis （FEA）. A microstructural unit cell for FEA is created to simulate a representative laminates unit with one pin. Within the unit cell, the first directions of the elements＇ material coordinate systems are changed to simulate the fibres＇ deflecting around the pin. The hygrothermal effect is simulated by the material properties＇ adjustments which are determined by the compressive tests of non-pined laminates. The experimental results indicate that the percentage of reduction in the compressive modulus of Z-pinned laminates caused by Z-pin becomes smaller with the percentage of 0° fibres decreasing in the laminates; the compressive strength of quasi-isotropic laminates reduces and the percentage of the reduction in the compressive strength declines with Z-pin volume content increasing, and the moisture absorption ratio of the Z-pinned specimens is greater than that of the non-pinned specimens, because the cracks around Z-pin increase the moisture absorption. In addition, the simulations show that the deflection of fibres around Z-pin is the main factor for the reduction in the compressive strength of Z-pinned unidirectional laminates, the dilution of fibre volume content caused by resin-rich pocket is the principal factor for the decline in the compressive strength of Z-pinned quasi-istropic laminates, and the compressive strength of Z-pinned specimens in hygrothermal environment reduces as the result of superimposition of some factors, including the changes in material properties caused by hygrothermal environment, the deflection of fibres and the resin-rich pocket caused by Z-pin.
