目的:探讨经脐缘两孔法腹腔镜阑尾切除术的临床疗效。方法:回顾分析2017年7月至2020年6月为166例患者采用经脐缘两孔单器械打结法行腹腔镜阑尾切除术的临床资料。分别于脐缘上、下穿刺10 mm、5 mm Trocar,双极电凝阶梯式电凝阑尾系膜并...目的:探讨经脐缘两孔法腹腔镜阑尾切除术的临床疗效。方法:回顾分析2017年7月至2020年6月为166例患者采用经脐缘两孔单器械打结法行腹腔镜阑尾切除术的临床资料。分别于脐缘上、下穿刺10 mm、5 mm Trocar,双极电凝阶梯式电凝阑尾系膜并离断,系膜无需结扎。经腹壁一次性注射器9号针头将7号丝线带入腹腔,线尾留于体外,腹腔镜下单器械打结结扎阑尾根部,切除阑尾。分析手术时间、中转开腹、术后并发症等指标,以明确手术安全性与可行性。结果:164例患者经两孔法顺利完成手术,手术成功率98.80%。2例因回盲部粘连严重,无法游离暴露阑尾,其中1例增加一枚Trocar完成手术,另1例中转开腹。手术时间22~95 min,平均(51.02±12.74)min;术后无相关并发症发生,患者均治愈出院。住院3~8 d,平均(6.34±1.03)d。结论:在熟练掌握腹腔镜下单器械打结技术的条件下,经脐缘两孔法行腹腔镜阑尾切除术操作方便,成功率较高,创伤较小,临床可推广应用。展开更多
Hydrophobic mismatch between the hydrophobic length of membrane proteins and hydrophobic thickness of membranes is a crucial factor in controlling protein function and assembly.We combined fluorescence with circular d...Hydrophobic mismatch between the hydrophobic length of membrane proteins and hydrophobic thickness of membranes is a crucial factor in controlling protein function and assembly.We combined fluorescence with circular dichroism（CD） and attenuated total reflection infrared（ATR-IR） spectroscopic methods to investigate the behaviors of the peptide and lipids under hydrophobic mismatch using a model peptide from the fourth transmembrane domain of natural resistance-associated macrophage protein 1（Nramp1）,the phosphatidylcholines（PCs） and phosphatidylglycerols（PGs） with different lengths of acyl chains（14：0,16：0 and 18：0）.In all PG lipid membranes,the peptide forms stable α-helix structure,and the helix axis is parallel to lipid chains.The helical span and orientation hardly change in varying thickness of PG membranes,while the lipid chains can deform to accommodate to the hydrophobic surface of embedded peptide.By comparison,the helical structures of the model peptide in PC lipid membranes are less stable.Upon incorporation with PC lipid membranes,the peptide can deform itself to accommodate to the hydrophobic thickness of lipid membranes in response to hydrophobic mismatch.In addition,hydrophobic mismatch can increase the aggregation propensity of the peptide in both PC and PG lipid membranes and the peptide in PC membranes has more aggregation tendency than that in PG membranes.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.20973083,20934002)
文摘Hydrophobic mismatch between the hydrophobic length of membrane proteins and hydrophobic thickness of membranes is a crucial factor in controlling protein function and assembly.We combined fluorescence with circular dichroism（CD） and attenuated total reflection infrared（ATR-IR） spectroscopic methods to investigate the behaviors of the peptide and lipids under hydrophobic mismatch using a model peptide from the fourth transmembrane domain of natural resistance-associated macrophage protein 1（Nramp1）,the phosphatidylcholines（PCs） and phosphatidylglycerols（PGs） with different lengths of acyl chains（14：0,16：0 and 18：0）.In all PG lipid membranes,the peptide forms stable α-helix structure,and the helix axis is parallel to lipid chains.The helical span and orientation hardly change in varying thickness of PG membranes,while the lipid chains can deform to accommodate to the hydrophobic surface of embedded peptide.By comparison,the helical structures of the model peptide in PC lipid membranes are less stable.Upon incorporation with PC lipid membranes,the peptide can deform itself to accommodate to the hydrophobic thickness of lipid membranes in response to hydrophobic mismatch.In addition,hydrophobic mismatch can increase the aggregation propensity of the peptide in both PC and PG lipid membranes and the peptide in PC membranes has more aggregation tendency than that in PG membranes.
