新时代课程育人研究的兴起与使命

新时代党中央高度重视思想政治工作的课堂教学主渠道,我国高等教育界对于课程育人的相关研究蓬勃兴起。课程育人的基本任务是大力推动以“课程思政”为目标的课堂教学改革,当前对于课程育人的研究主要围绕“课程思政”建设展开。学者们围绕课程思政的内涵、价值与意义、实现路径、课程思政与“思政课程”的关系以及在具体课程中的实施等领域展开研究,形成了一些有价值的观点。历经“起步、发展”阶段,未来研究应突出重点,彰显新时代特征,尽快形成课程育人理论体系,并增强研究的可借鉴性和可操作性,进一步迈向“深入”阶段,将课程育人研究做细、做深、做实。

肝硬化合并自发性细菌性腹膜炎患者腹水病原学及耐药性分析

目的分析活动性肝硬化并发自发性腹膜炎（SBP）患者腹水培养的细菌学及耐药变化趋势，为抗菌药物合理应用提供依据。方法选择2012年1月至2016年12月天津市第二人民医院住院治疗的诊断符合SBP且腹水细菌学培养阳性患者140例，根据腹水培养结果将患者分为革兰阳性（G^＋）菌感染组50例和革兰阴性（G-）菌感染组90例。分析其临床资料、细菌学资料及耐药情况。结果从2012至2016年G^＋菌感染在SBP细菌谱中的例数呈逐年上升趋势（分别为3、4、10、12、21例），SBP条件致病菌比例逐年增多，从2012年的33．3％增长到2016年的41．9％。G^＋菌感染组血中性粒细胞比例（N）、腹水白细胞计数（WBC）、多核粒细胞数、降钙素原（PCT）均较G-菌感染组明显减少（均P〈0．05）。从2012年至2016年G菌感染以大肠埃希菌为主占50．O％（45例），其中耐药率较低的3种抗菌药物为头孢哌酮／舒巴坦（0）、亚胺培南（3．33％）、阿米卡星（7．78％），头孢菌素类耐药率最高，依次是头孢唑林、头孢呋辛钠、头孢他啶分别为47．78％、38．89％、34．44％；G^＋菌感染葡萄球菌33例（占64．7％）、肠球菌13例（占25．5％）、链球菌4例（占7．8％），耐药率较低的3种抗菌药物为替加环素、利奈唑胺、万古霉素分别为0、4．0％、8．0％，葡萄球菌对利奈唑胺和替加环素的耐药率均为0，肠球菌对替加环素的耐药率为0。结论对于SBP患者，致病菌仍以菌为主，但G^＋菌感染比例呈上升趋势；治疗首选的药物包括碳青霉烯类的亚胺培南和β-内酰胺类抗菌药与β-内酰胺酶抑制剂合剂头孢哌酮／舒巴坦，肾功能较好者可加用阿米卡星；经验治疗效果欠佳，需要考虑应用抗G^＋菌的药物。

牛体细胞核移植胚胎的批量化生产

利用2枚显微去核后的半卵与1枚供体细胞同步融合,并结合微穴法(WOWs)培养体系批量化生产成年牛克隆胚胎(日产40～80枚).结果,重构胚电融合率95.7%(3059/3197)、卵裂率87.1%(2637/3027)、囊胚率41.1%(1244/3027)和可冻胚率(72.5%,933/1244)均达到较高水平.克隆胚胎采用玻璃微管玻璃化冷冻保存数月后移植,30日龄时妊娠率为28.1%(48/171),已经产下5头足月克隆牛续.结果表明,该方法具有产业开发潜力.

头孢他啶与5种止血药在输液中配伍的稳定性考察

目的 考察头孢他啶与 5种止血药 (维生素K1、酚磺乙胺、氨甲苯酸、氨甲环酸、氨基己酸 )在 3种输液中配伍后的稳定性。方法 分别采用紫外分光光度法、系数倍率法及双波长分光光度法观察头孢他啶与 5种止血药配伍后在 5℃、2 0℃、37℃下 ,4h内头孢他啶含量及混合液的pH和外观的变化。结果 头孢他啶与 5种止血药在NS、5 %GS、GNS中配伍后 ,在不同温度下4小时内含量及混合液pH均无显著变化。结论 注射用头孢他啶与 5种止血药在 3种输液中在 4h内可配伍应用。

改良式颈动脉内膜剥脱术对重度颈动脉狭窄的早期疗效分析

目的评价在传统的颈动脉内膜剥脱术基础上进行术式改良的改良式颈动脉内膜剥脱术治疗重度颈动脉狭窄的疗效以及早期结局。方法回顾性分析2018年3月至2019年4月南方医院血管外科采用改良式颈动脉内膜剥脱术治疗7例重度颈动脉狭窄患者的临床资料。结果共行7次改良式颈动脉内膜剥脱术,手术均获得成功,颈动脉平均阻断时间为(14.1±4.4)min。术后发生轻症脑梗死1例,未发生切口血肿、颅神经损伤、颅脑高灌注、心肌梗死或死亡。术后改良Rankin量表评分0分1例,1分6例,结果较术前有所改善(t=2.500,P<0.05)。结论改良式颈动脉内膜剥脱术能避免颈内动脉因血管缝合导致的管腔丢失,保证术后通畅率,术后并发症发生无明显升高,但仍需大样本和远期随访进行验证。

Analysis of Wave Loads on A Semi-Submersible Platform

For the global and structural fatigue strength analysis of a semi-submersible platform, wave loads under design conditions are calculated by use of the three-dimensional boundary element method. Methods for calculating the forward-speed free-surface Green function are discussed and a computer program with this Green function is developed. According to the special rules, the wave loads under several typical design conditions of the platform are calculated. The maximum vertical bending moment, torsion moment and horizontal split force are determined from a series of contour maps of wave loads for the wave period of 5 to 18 seconds at a certain interval and the wave phase of 0degrees to 360degrees at a certain interval. The wave height is determined by the function of wave period with a given exceedance probability. The maximum wave loads under the combination of wave parameters are used as the input of hydrodynamic pressure in the three-dimensional finite element analysis process. The transfer functions of wave loads on the platform are used for the fatigue strength analysis of the K-tubular joint and the sub-model of the structure.

Notes Effects of four nitrogen substrates on growth of several red tide species

Eight common red tide species including the Dinoflagellates Alejcandrium tamarense,Prorocentrum minimum , Prorocentrum mican , Diatoms Skeletonema costatum , Cerataulina pelag-ica, Leptocylindrus minimus, a Raphidophyte Heterosigma akashiwo and a Chrysophyte Isochrysis galbana were chosen to study the effect of four nitrogen substrates (NOs ?N, NHi - N, Urea ?N, Mixed ?N) on the growth of these algae. After two transfers, the results showed that at N concentration of 550 fano\, NO; - N was the best nitrogen source among these four nitrogen substrates; Urea - N and Mixed ~ N were also good for the growth of most algae, but not as good as NQj ?N for some species; NH??N inhibited the growth of all these species except H. akashrwo. At concentrations of 280 and 50 fimol experiments on the growth of /. galbana , P. minimum and L. minimus in Nri( - N and NO; - N substrates were also performed. The results showed that the growth rates of /. galbana were not significantly different from each other in NO; - N and NH?- N substrates both concentrations of 280 and 50 fonol. In concentration of 280 (anol NHt - N substrate, the growth rates of P. minimum and L. minimus were slower than in same concentration of NO? ~~ N; At concentration of 50 (unol , the growth rate of P. minimum in NH?- N was not significantly different from that in NOj ?N, while the growth rate of L. minimus was only about 30% of that in NQ3 ~ N substrate. The results indicated that each alga had its own preference in N-substrate and concentration, therefore, different nitrogen substrates may play a role in red tide formation.

The coda attenuation of the Yao'an area in Yunnan Province

An earthquake with M=6.5 happened on January 15, 2000 in Yaoan of Yunnan Province. After the earthquake, a temporary digital network with 6 detectors around the epicenter area was set up. 402 aftershocks were located more precisely. According to coda short recording observed, the coda averaging quality factor has been acquired via Satos single scattering model analyses, Qc( f )=49f 0.95,f=1.5~20.0 Hz, which has the attenuation characteristics of high structural active region.

Study on Active Drive System for Colonoscope

The paper deals with active drive system for colonoscope. The system is mainly composed of soft mobile mechanism for earthworm locomotion and turning mechanism based on shape memory effect. The soft mobile mechanism contacts colon wall with air in inflatable balloons, so the robot has better soft and non invasive properties. The turning mechanism can be actively bent by shape memory alloy components. It ensures the colonoscope to adapt to the tortuous shape of colon. Some experiment results are given in the paper.

A PREDICTOR-CORRECTOR INTERIOR-POINT ALGORITHM FOR CONVEX QUADRATIC PROGRAMMING

The simplified Newton method, at the expense of fast convergence, reduces the work required by Newton method by reusing the initial Jacobian matrix. The composite Newton method attempts to balance the trade-off between expense and fast convergence by composing one Newton step with one simplified Newton step. Recently, Mehrotra suggested a predictor-corrector variant of primal-dual interior point method for linear programming. It is currently the interiorpoint method of the choice for linear programming. In this work we propose a predictor-corrector interior-point algorithm for convex quadratic programming. It is proved that the algorithm is equivalent to a level-1 perturbed composite Newton method. Computations in the algorithm do not require that the initial primal and dual points be feasible. Numerical experiments are made.

Study on medium-short term earthquake forecast in Yunnan Province by precursory events

The medium-short term forecast for a certain kinds of main earthquake events might be possible with the time-to-failure method presented by Varnes (1989), Bufe and Varnes (1993), which is to simulate an accelerative releasing model of precursory earthquake energy. By fitting the observed data with the theoretical formula, a medium-short term forecast technique for the main shock events could be established, by which the location, time and magnitude of the main shock could be determined. The data used in the paper are obtained from the earthquake catalogue recorded by Yunnan Regional Seismological Network with a time coverage of 1965~2002. The statistical analyses for the past 37 years show that the data of M2.5 earthquakes were fairly complete. In the present paper, 30 main shocks occurred in Yunnan region were simulated. For 25 of them, the forecasting time and magnitude from the simulation of precursory sequence are very close to the actual values with the precision of about 0.57 (magnitude unit). Suppose that the last event of the precursory sequence is known, then the time error for the forecasting main shock is about 0.64 year. For the other 5 main shocks, the simulation cannot be made due to the insufficient precursory events for the full determination of energy accelerating curve or disturbance to the energy-release curve. The results in the paper indicate that there is no obviously linear relation in the optimal searching radius for the main shock and the precursory events because Yunnan is an active region with damage earthquakes and moderate and small earthquakes. However, there is a strong correlation between the main shock moment and the coefficient k/m. The optimal fitting range for the forecasting time and magnitude can be further reduced using the relation between the main shock moment lgM0 and the coefficient lgk/m and the value range of the restricting index m, by which the forecast precision of the simulated main shock can be improved. The time-to-failure method is used to fit 30 main shocks in the paper and more than 80% of them have acquired better results, indicating that the method is prospective for its ability to forecast the known main shock sequence. Therefore, the prospect is cheerful to make medium-short term forecast for the forthcoming main shocks by the precursory events.

In-vitro maturation of human germinal vesicle stage oocytes cultured with human follicular fluid:a report of 3 pregnancies

In-vitro maturation (IVM) of human oocyte is an assisted reproductive technique , which has been used in clinic for more than 10 years since the first report by Cha 1991[1]. Many clinical trials have indicated that IVM technology is not only used to treat infertile women with stubborn polycystic ovarian syn drome (PCOS)[2], to prevent patients from ovarian hyperstimulation syn drome (OHSS)[3],and to rescue the poor developed oocytes in stimu lated cycles , but also to reduce the cost and to avert the side-effects of gonadotropin sti mulation for in-vitro fertilization (IVF). Although there were lots of studies involving to the competence of the immature oocyte from mammalian or human ovar ies to become mature in vitro, the pregnancy rate from IVM is much lower than th at of IVF as reported[4]. This indicates that optimization of IVM remains a challenge. ……

Establishment of animal model for potency evaluationof inactivated SARS virus experimental vaccine

The purpose of this study was to test the effectiveness of source virus strain for the manufacture of the inactivated SARS virus vaccine, and establish an experimental method and preliminary standard for potency evaluation. Mice were divided into groups for being immunized with corresponding serially diluted experimental SARS virus inactivated vaccine. And the rabbits were immunized with undiluted vaccine. Challenge assay was conducted with a heterologous SARS virus. And the neutralization antibody was determined with plaque reduction neutralization test (PRNT), to which the neutralization antibody in the convalescent serum of SARS patients was compared. The experimental vaccine viral strains were proved to be suitable for manufacturing the vaccine. Mice immunized by vaccines of serial dilutions were able to elicit neutralizing antibody. The antibody titer from mice immunized with the undiluted vaccine could reach up to 1∶495.2, while those of rabbits immunized with the undiluted vaccine could reach a GMT of 55.0-79.9. The capability of the antibody to neutralize the virus from Guangdong is more efficient than that from Beijing. The GMT of neutralizing antibody in SARS convalescents living in south and north China ranged from 50.12 to 54.95, and the titers of convalescents from north China were higher than those from south China. Mice and rabbits used as the model for evaluation of potency are of sensitivity, and the test is of reproducibility. The candidate challenge viral strains showed a relatively consistent effect on evaluating antibodies produced by various batches and different vaccine-source strains, hence they can be used to evaluate potency of the vaccine. The method for testing the vaccine potency and the evaluation standard was established preliminarily.

CONSTRUCTION OF INTEGRATED CAD TOOLS ENVIRONMENT TO SUPPORT CONCURRENT COLLABORATIVE DESIGN

A CAD tools environment is described to support concurrent collaborative design. The environment has four components: shared design representation, design process control, CAD tools, designer interface. Some related issues for the construction, such as form feature handling, constraint satisfaction, design process control, STEP based information integration, are discussed in more detail.

DEVELOPMENT OF A COUPLED REGIONAL AIR-SEA MODEL AND ITS NUMERICAL SIMULATION FOR SUMMER MONSOON IN 1998

A coupled regional air-sea model is developed by using the regional climate model (P-σ RCM) and the regional ocean model (POM),which is used to simulate East Asian monsoon and oceanic elements in East Asian coastal waters.The simulated surface layer oceanic elements are basically consistent with the reality and can reflect the interaction between the monsoon and the surface layer currents.The great difference with the reality is “cold drift” of the simulated surface temperature.The coupled model has certain ability to simulate the atmosphere geopotential height fields,precipitation and low-level southwest wind from May to August in 1998.It can display the process of summer monsoon onset during the third dekad of May and the evolution features after the onset.The differences between the simulation results of the coupled model and that of the single P-a RCM are shown mainly in the low-level atmosphere and the model internal regions.

Study on Therapeutic Mechanism of Anti-Rheumatism Effect of Siegesbeckia Pubescens

Objective: To explore the mechanism of anti-rheumatic effect of the active fraction of Siegesbeckia pubescens (AFSP). Methods: Adjuvant arthritis (AA) model of rat was produced to observe the effect of AFSP on lymphocyte proliferation, interleukin-1 and -2 (IL-1,-2) activity, pathologic section of ankle joint, and analgesic effect, in model rat. Results: AFSP could reduce the inflammatory pathologic response of ankle joint. It has good analgesic effect, the analgesic rate being 65%. AFSP could also strengthen T-lymphocyte proliferation, promote IL-2 activity and inhibit IL-1 activity. Compared with the control group, the difference was significant (P<0.01). Conclusion: By means of regulating the immune function of organism, AFSP could improve the local pathologic response to antagonize against rheumatism, therefore, it is an effective anti-rheumatism herbal medicine.

SEASONAL VARIATIONS OF CONVECTIVE ACTIVITIES OVER THE SOUTH CHINA SEA AND ITS NEIGHBORHOOD AND THEIR COMPARATIVE ANALYSES IN THE STRONG AND WEAK CONVECTION YEARS

The seasonal variations of convective activities over the South China Sea(SCS)and its neighborhood.as well as the similarities and differences of convection in the different key regions during the strong and weak convection years are analyzed by using the pentad data of TBB from 1980 to 1993.The results show that in winter and summer the seasonal variations of the convective activities are synchronous over the SCS and its neighborhood,the anomalous convection amplitudes are obviously different in different regions.The significant extents of convective activities have somewhat seasonal differences in the strong and weak convection years.In the strong convection years,it is in winter,spring and autumn that the convection anomaly is more evident than that in the normal years,however,after the summer monsoon onset the convection is sustained.stable and similar to that in the normal years.In the weak convection years.the convection weakens greatly in each season.but the primary weakening occurs in spring.summer and autumn.No matter in the strong or the weak convection years.the convective activities are somewhat of difference in the Bay of Bengal.the Indochina Peninsula.the SCS and the Philippines.In addition. the convective activities are also different over the south and the north parts of the SCS.the convection variation in the strong year is similar to that in the weak year over the north part of the SCS.but over the south part there are great differences.

降低血浆中的低密度脂蛋白（LDL）

PCSK9是一种蛋白酶，能调控低密度脂蛋白受体（LDLR）中的蛋白质水平。进而控制血浆中堤密度脂蛋白（LDL）中的胆固醇水平。所以，PCSK9是一种通过降低LDL胆固醇水平来减少动脉粥样硬化、心脏病、中风及外周血管疾病等疾病风险的蛋白。