A real-time label-free lateral offset spliced coreless fiber(CF)Mach-Zehnder interferometer(MZI)biosensor functionalized with hydrophobin Grifola frondosa I(HGFI)was proposed for the detection of cytokine tumour necro...A real-time label-free lateral offset spliced coreless fiber(CF)Mach-Zehnder interferometer(MZI)biosensor functionalized with hydrophobin Grifola frondosa I(HGFI)was proposed for the detection of cytokine tumour necrosis factor alpha(TNF-α).The nanolayer self-assembled on the optical fiber surfaces by HGFI rendered the immobilization of probe TNF-αantibody and recognition of antigen TNF-α.Trifluoroacetic acid was utilized to remove the HGFI layer from the glass surface,which was validated by field emission scanning electron microscopy(FESEM)and water contact angle(WCA).Results demonstrated that the processes of HGFI modification,antibody immobilization and specific antibody detection can be monitored in real time.The proposed biosensor exhibited good specificity,repeatability and low detection limit for TNF-α,extending its application in inflammation and disease monitoring.展开更多
基金Supported by the National Natural Science Foundation of China(31770102)Tianjin Manicipal Science and Technology Bureau(21JCQNJC00110)+1 种基金Tianjin Manicipal Health Commission(KJ20070)Chun Foundation of Tianjin First Central Hospital(2020CM12)。
基金supported by the Sino-Swiss Scientific and Technological Cooperation Project supported by the Ministry of Science and Technology of China(No.2015DFG32140)the National Key Research and Development Program of China(Nos.2018YFA0900100 and 2018YFB1802302)+2 种基金the National Natural Science Foundation of China(Nos.11774181,11804250,11904180,11904262,61875091 and 62105164)the Natural Science Foundation of Tianjin City(Nos.19JCYBJC16700,20JCQNJC01480,21JCQNJC00210 and 21JCYBJC00080)the Tianjin Education Commission Scientific Research Project(Nos.2018KJ146 and 2019KJ016).
文摘A real-time label-free lateral offset spliced coreless fiber(CF)Mach-Zehnder interferometer(MZI)biosensor functionalized with hydrophobin Grifola frondosa I(HGFI)was proposed for the detection of cytokine tumour necrosis factor alpha(TNF-α).The nanolayer self-assembled on the optical fiber surfaces by HGFI rendered the immobilization of probe TNF-αantibody and recognition of antigen TNF-α.Trifluoroacetic acid was utilized to remove the HGFI layer from the glass surface,which was validated by field emission scanning electron microscopy(FESEM)and water contact angle(WCA).Results demonstrated that the processes of HGFI modification,antibody immobilization and specific antibody detection can be monitored in real time.The proposed biosensor exhibited good specificity,repeatability and low detection limit for TNF-α,extending its application in inflammation and disease monitoring.
