Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred...Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six deaths.To clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells.The complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number KP012546.Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade.No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014.The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,China.These results help clarify the origin of this epidemic and predict the future status of dengue in China.展开更多
China is experiencing the most severe dengue outbreak since the 1980s. Till 31 October 2014, a total of 44497 la- boratory-confirmed dengue cases with six deaths have been reported in China's Mainland, among which...China is experiencing the most severe dengue outbreak since the 1980s. Till 31 October 2014, a total of 44497 la- boratory-confirmed dengue cases with six deaths have been reported in China's Mainland, among which 43031 cases were from Guangdong. Although dengue fever is classified as a notifiable infectious disease according to Chinese Ministry of Health, the public and health care system has not fully appreciated the medical burden of this disease. The unprec- edented dengue cases in China have set off the alarm for public health to respond to emerging and re-emerging dis- eases. A comprehensive strategy is needed to win the battle against dengue and other infectious diseases, including sur- veillance, diagnostics, vector control, vaccine, and drug development.展开更多
基金supported by Guangzhou Science and Technology Program for Public Wellbeing(2014Y2-00185)the Special Program of National Science and Technology of China(2013ZX10004-805)the National Natural Science Foundation of China(81301491 and 31270974)
文摘Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six deaths.To clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells.The complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number KP012546.Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade.No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014.The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,China.These results help clarify the origin of this epidemic and predict the future status of dengue in China.
文摘China is experiencing the most severe dengue outbreak since the 1980s. Till 31 October 2014, a total of 44497 la- boratory-confirmed dengue cases with six deaths have been reported in China's Mainland, among which 43031 cases were from Guangdong. Although dengue fever is classified as a notifiable infectious disease according to Chinese Ministry of Health, the public and health care system has not fully appreciated the medical burden of this disease. The unprec- edented dengue cases in China have set off the alarm for public health to respond to emerging and re-emerging dis- eases. A comprehensive strategy is needed to win the battle against dengue and other infectious diseases, including sur- veillance, diagnostics, vector control, vaccine, and drug development.
