Biogas slurry is a traditional high-quality organic liquid fertilizer. Three terrestrial plants with high economic value, fu </span><span style="font-family:Verdana;">gui</span> <span st...Biogas slurry is a traditional high-quality organic liquid fertilizer. Three terrestrial plants with high economic value, fu </span><span style="font-family:Verdana;">gui</span> <span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;">, yang </span><span style="font-family:Verdana;">xin</span> <span style="font-family:Verdana;">cai</span> <span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> strawberry, were selected for hydroponic cultivation using an optimal dilution of biogas slurry. The results of the experiments showed that strawberries could bloom and bear fruit and fu </span><span style="font-family:Verdana;">gui</span> <span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;"> and yang </span><span style="font-family:Verdana;">xin</span> <span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;"> grew well. However, in comparison with the control groups planted in soil, all three crops were subject to a certain degree of environmental stress, as shown by changes in growth, biomass indicators, physiological indicators </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> morphological indicators. The leaves and stems of yang </span><span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;"> and fu </span><span style="font-family:Verdana;">gui</span> <span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;"> were tested for several types of heavy metals, and all met the requirements for human consumption. It is feasible to cultivate these three plants with biogas slurry. The results of this study can help guide hydroponic production practices and have practical significance and reference value.展开更多
Cytochrome P450s(CYPs)are the largest family of enzymes in plant and play multifarious roles in development and defense but the available information about the CYP superfamily in citrus is very limited.Here we provide...Cytochrome P450s(CYPs)are the largest family of enzymes in plant and play multifarious roles in development and defense but the available information about the CYP superfamily in citrus is very limited.Here we provide a comprehensive genome-wide analysis of the CYP superfamily in Citrus clementina genome,identifying 301 CYP genes grouped into ten clans and 49 families.The characteristics of both gene structures and motif compositions strongly supported the reliability of the phylogenetic relationship.Duplication analysis indicated that tandem duplication was the major driving force of expansion for this superfamily.Promoter analysis revealed numerous cis-acting elements related to various responsiveness.RNA-seq data elucidated their expression patterns in citrus fruit peel both during development and in response to UV-B.Furthermore,we characterize a UV-B-induced CYP gene(Ciclev10019637m,designated CitF3′H)as a f lavonoid 3-hydroxylase for the first time.CitF3 H catalyzed numerous f lavonoids and favored naringenin in yeast assays.Virusinduced silencing of CitF3′ H in citrus seedlings significantly reduced the levels of 3′-hydroxylated f lavonoids and their derivatives.These results together with the endoplasmic reticulum-localization of CitF3 H in plant suggest that this enzyme is responsible for the biosynthesis of 3-hydroxylated f lavonoids in citrus.Taken together,our findings provide extensive information about the CYP superfamily in citrus and contribute to further functional verification.展开更多
[Objectives]To observe the clinical effect of Huatanjieyu granule on patients with post-stroke depression.[Methods]Total 76 patients with confirmed post-stroke depression were randomly divided into two groups(treatmen...[Objectives]To observe the clinical effect of Huatanjieyu granule on patients with post-stroke depression.[Methods]Total 76 patients with confirmed post-stroke depression were randomly divided into two groups(treatment group and control group)according to the double-blind principle.The treatment group(38 cases)was treated with Huatanjieyu granule combined with escitalopram,and the control group(38 cases)was treated with escitalopram alone.The course of treatment was 6 weeks.Through clinical efficacy,Hamilton Depression Scale(HAMD)and Treatment Emergent Symptom Scale(TESS)scores were compared,and blood,liver function,renal function and electrocardiogram were examined.[Results]The scores of HAMD and TESS in patients with post-stroke depression were significantly decreased by Huatanjieyu granule,and there were no obvious side effects.The total effective rate was 92.1%in the treatment group and 76.3%in the control group.There was significant difference between the two groups(P<0.01).[Conclusions]Huatanjieyu granule was effective in the treatment of post-stroke depression with little side effects and was easy to be accepted by patients.展开更多
Polymethoxylated flavones(PMFs),the main form of flavones in citrus,are derived from the flavone branch of the flavonoid biosynthesis pathway.Flavone synthases(FNSs)are enzymes that catalyze the synthesis of flavones ...Polymethoxylated flavones(PMFs),the main form of flavones in citrus,are derived from the flavone branch of the flavonoid biosynthesis pathway.Flavone synthases(FNSs)are enzymes that catalyze the synthesis of flavones from flavanones.However,the FNS in citrus has not been characterized yet.Here,we identified two type II FNSs,designated CitFNSII-1 and CitFNSII-2,based on phylogenetics and transcriptome analysis.Both recombinant CitFNSII-1 and CitFNSII-2 proteins directly converted naringenin,pinocembrin,and liquiritigenin to the corresponding flavones in yeast.In addition,transient overexpression of CitFNSII-1 and CitFNSII-2,respectively,in citrus peel significantly enhanced the accumulation of total PMFs,while virus-induced CitFNSII-1 and CitFNSII-2 genes silencing simultaneously significantly reduced the expression levels of both genes and total PMF content in citrus seedlings.CitFNSII-1 and CitFNSII-2 presented distinct expression patterns in different cultivars as well as different developmental stages.Methyl salicylate(MeSA)treatment reduced the CitFNSII-2 expression as well as the PMFs content in the peel of Citrus sinensis fruit but did not affect the CitFNSII-1 expression.These results indicated that both CitFNSII-1 and CitFNSII-2 participated in the flavone biosynthesis in citrus while the regulatory mechanism governing their expression might be specific.Our findings improved the understanding of the PMFs biosynthesis pathway in citrus and laid the foundation for further investigation on flavone synthesis regulation.展开更多
[Objectives]This study aimed to study the sleep-improving effect of Anshen Babu ointment on insomnia mice and its mechanism of action.[Methods]Mouse models of insomnia were established by p-chlorophenylalanine(PCPA)in...[Objectives]This study aimed to study the sleep-improving effect of Anshen Babu ointment on insomnia mice and its mechanism of action.[Methods]Mouse models of insomnia were established by p-chlorophenylalanine(PCPA)injection.They were given high,middle and low doses of Anshen Babu ointment,for 7 consecutive days,and then,the spontaneous activity of insomnia mice and the effect on hypnotic time and subthreshold hypnotic dose of pentobarbital sodium were observed,and the levels of 5-HT,IL-6 and TNF-α in the hippocampus of mice were determined.[Results]High and middle-dose Anshen Babu ointment significantly reduced the spontaneous activity of insomnia mice and shorten the hypnotic time of pentobarbital sodium;high and middle-dose Anshen Babu ointment increased the 5-HT content and inhibited the IL-6 and TNF-α expression in the hippocampus of mice.[Conclusions]Anshen Babu ointment has hypnotic and sedative effect on insomnia mice.The mechanism may be achieved by increasing 5-HT and reducing IL-6 and TNF-α levels.展开更多
[Objectives]This study aimed to observe the clinical efficacy of sedative cataplasm in the treatment of insomnia patients with sub-health.[Methods]A total of 90 insomnia patients with sub-health were randomly and even...[Objectives]This study aimed to observe the clinical efficacy of sedative cataplasm in the treatment of insomnia patients with sub-health.[Methods]A total of 90 insomnia patients with sub-health were randomly and evenly divided into treatment group and control group.The patients in the control group were given Bozi Yangxin pills orally,and those in the treatment group were treated with sedative cataplasm on Shenjue acupoint.The treatment duration was 4 weeks.The Pittsburgh Sleep Quality Index(PSQI)was used to evaluate clinical efficacy.[Results]The overall efficacy of the treatment group and control group was 91.1%and 77.1%,respectively,and the difference between the two was significant(P<0.05).After treatment,the PSQI scores of each group changed significantly(P<0.05).[Conclusions]Sedative cataplasm has a good clinical efficacy on sub-healthy insomnia patients.展开更多
Masterpieces of world literature, classical or contemporary, have been available in Chinese translation since the beginning of the 20th century. They outweigh by far the translations of Chinese literary works. Oversea...Masterpieces of world literature, classical or contemporary, have been available in Chinese translation since the beginning of the 20th century. They outweigh by far the translations of Chinese literary works. Overseas literature has had perceptible influence on Chinese modern literature and performance arts. The following ten writers and playwrights are considered leading foreign literary masters by Chinese lovers of non-Chinese literature.展开更多
Adenine is commonly used to establish the animal models for chronic kidney injury and its renal interstitial fibrosis. As an endogenous substance, adenine-induced kidney damage has not yet been fully studied and eluci...Adenine is commonly used to establish the animal models for chronic kidney injury and its renal interstitial fibrosis. As an endogenous substance, adenine-induced kidney damage has not yet been fully studied and elucidated, except for inflammatory reaction. Here we analyzed the proteomics of kidney of rats after adenine overloading using LS-MS/MS assay, and observed the role of anemoside B4(B4). The results showed that adenine could down-regulate 285 proteins and up-regulate 164 proteins in rat kidney tissue compared with the normal group. Down-regulated proteins mainly affected related pathways, such as energy metabolism, while up-regulated proteins affected inflammatory response pathways and metabolic pathways. B4 could significantly reverse the down-regulation of about 40 proteins, which were involved in mitochondria, redox processes, extracellular exosomes, acetylation and other signaling pathways. Simultaneously, B4 could inhibit the up-regulation of five proteins caused by adenine, which were involved in cell cycle, oocyte meiosis, PI3 K-Akt and other signaling pathways. Further experimental results of mRNA expression using real-time PCR assay supported the proteomic analysis. Therefore, we proposed that the damage of rat kidney caused by adenine was more complicated, not only with an inflammatory reaction, but also with extensive effects to various metabolic processes in the body. This work provided a valuable clue for comprehensive understanding of adenine-induced renal damage.展开更多
文摘Biogas slurry is a traditional high-quality organic liquid fertilizer. Three terrestrial plants with high economic value, fu </span><span style="font-family:Verdana;">gui</span> <span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;">, yang </span><span style="font-family:Verdana;">xin</span> <span style="font-family:Verdana;">cai</span> <span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> strawberry, were selected for hydroponic cultivation using an optimal dilution of biogas slurry. The results of the experiments showed that strawberries could bloom and bear fruit and fu </span><span style="font-family:Verdana;">gui</span> <span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;"> and yang </span><span style="font-family:Verdana;">xin</span> <span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;"> grew well. However, in comparison with the control groups planted in soil, all three crops were subject to a certain degree of environmental stress, as shown by changes in growth, biomass indicators, physiological indicators </span><span style="font-family:Verdana;">and</span><span style="font-family:Verdana;"> morphological indicators. The leaves and stems of yang </span><span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;"> and fu </span><span style="font-family:Verdana;">gui</span> <span style="font-family:Verdana;">cai</span><span style="font-family:Verdana;"> were tested for several types of heavy metals, and all met the requirements for human consumption. It is feasible to cultivate these three plants with biogas slurry. The results of this study can help guide hydroponic production practices and have practical significance and reference value.
基金supported by the China Postdoctoral Science Foundation(2021 M692845 and 2021 M700124)the National Natural Science Foundation of China(32101932 and 32072132)the Fundamental Research Funds for the Central Universities(K20220104 and 226-2022-00215).
文摘Cytochrome P450s(CYPs)are the largest family of enzymes in plant and play multifarious roles in development and defense but the available information about the CYP superfamily in citrus is very limited.Here we provide a comprehensive genome-wide analysis of the CYP superfamily in Citrus clementina genome,identifying 301 CYP genes grouped into ten clans and 49 families.The characteristics of both gene structures and motif compositions strongly supported the reliability of the phylogenetic relationship.Duplication analysis indicated that tandem duplication was the major driving force of expansion for this superfamily.Promoter analysis revealed numerous cis-acting elements related to various responsiveness.RNA-seq data elucidated their expression patterns in citrus fruit peel both during development and in response to UV-B.Furthermore,we characterize a UV-B-induced CYP gene(Ciclev10019637m,designated CitF3′H)as a f lavonoid 3-hydroxylase for the first time.CitF3 H catalyzed numerous f lavonoids and favored naringenin in yeast assays.Virusinduced silencing of CitF3′ H in citrus seedlings significantly reduced the levels of 3′-hydroxylated f lavonoids and their derivatives.These results together with the endoplasmic reticulum-localization of CitF3 H in plant suggest that this enzyme is responsible for the biosynthesis of 3-hydroxylated f lavonoids in citrus.Taken together,our findings provide extensive information about the CYP superfamily in citrus and contribute to further functional verification.
基金the Hospital-level Project of Shiyan Taihe Hospital in 2018(2018JJXM092)Shiyan Science and Technology Research and Development Project in 2018(18Y17).
文摘[Objectives]To observe the clinical effect of Huatanjieyu granule on patients with post-stroke depression.[Methods]Total 76 patients with confirmed post-stroke depression were randomly divided into two groups(treatment group and control group)according to the double-blind principle.The treatment group(38 cases)was treated with Huatanjieyu granule combined with escitalopram,and the control group(38 cases)was treated with escitalopram alone.The course of treatment was 6 weeks.Through clinical efficacy,Hamilton Depression Scale(HAMD)and Treatment Emergent Symptom Scale(TESS)scores were compared,and blood,liver function,renal function and electrocardiogram were examined.[Results]The scores of HAMD and TESS in patients with post-stroke depression were significantly decreased by Huatanjieyu granule,and there were no obvious side effects.The total effective rate was 92.1%in the treatment group and 76.3%in the control group.There was significant difference between the two groups(P<0.01).[Conclusions]Huatanjieyu granule was effective in the treatment of post-stroke depression with little side effects and was easy to be accepted by patients.
基金This research was supported by the China Postdoctoral Science Foundation(2021 M700124 and 2021 M692845)the National Natu-ral Science Foundation of China(32072132)the Fundamental Research Funds for the Central Universities(K20220104).
文摘Polymethoxylated flavones(PMFs),the main form of flavones in citrus,are derived from the flavone branch of the flavonoid biosynthesis pathway.Flavone synthases(FNSs)are enzymes that catalyze the synthesis of flavones from flavanones.However,the FNS in citrus has not been characterized yet.Here,we identified two type II FNSs,designated CitFNSII-1 and CitFNSII-2,based on phylogenetics and transcriptome analysis.Both recombinant CitFNSII-1 and CitFNSII-2 proteins directly converted naringenin,pinocembrin,and liquiritigenin to the corresponding flavones in yeast.In addition,transient overexpression of CitFNSII-1 and CitFNSII-2,respectively,in citrus peel significantly enhanced the accumulation of total PMFs,while virus-induced CitFNSII-1 and CitFNSII-2 genes silencing simultaneously significantly reduced the expression levels of both genes and total PMF content in citrus seedlings.CitFNSII-1 and CitFNSII-2 presented distinct expression patterns in different cultivars as well as different developmental stages.Methyl salicylate(MeSA)treatment reduced the CitFNSII-2 expression as well as the PMFs content in the peel of Citrus sinensis fruit but did not affect the CitFNSII-1 expression.These results indicated that both CitFNSII-1 and CitFNSII-2 participated in the flavone biosynthesis in citrus while the regulatory mechanism governing their expression might be specific.Our findings improved the understanding of the PMFs biosynthesis pathway in citrus and laid the foundation for further investigation on flavone synthesis regulation.
基金Science and Technology Research and Development Plan of Shiyan City(16Y76).
文摘[Objectives]This study aimed to study the sleep-improving effect of Anshen Babu ointment on insomnia mice and its mechanism of action.[Methods]Mouse models of insomnia were established by p-chlorophenylalanine(PCPA)injection.They were given high,middle and low doses of Anshen Babu ointment,for 7 consecutive days,and then,the spontaneous activity of insomnia mice and the effect on hypnotic time and subthreshold hypnotic dose of pentobarbital sodium were observed,and the levels of 5-HT,IL-6 and TNF-α in the hippocampus of mice were determined.[Results]High and middle-dose Anshen Babu ointment significantly reduced the spontaneous activity of insomnia mice and shorten the hypnotic time of pentobarbital sodium;high and middle-dose Anshen Babu ointment increased the 5-HT content and inhibited the IL-6 and TNF-α expression in the hippocampus of mice.[Conclusions]Anshen Babu ointment has hypnotic and sedative effect on insomnia mice.The mechanism may be achieved by increasing 5-HT and reducing IL-6 and TNF-α levels.
基金Science and Technology Research and Development Project of Shiyan City(16Y76).
文摘[Objectives]This study aimed to observe the clinical efficacy of sedative cataplasm in the treatment of insomnia patients with sub-health.[Methods]A total of 90 insomnia patients with sub-health were randomly and evenly divided into treatment group and control group.The patients in the control group were given Bozi Yangxin pills orally,and those in the treatment group were treated with sedative cataplasm on Shenjue acupoint.The treatment duration was 4 weeks.The Pittsburgh Sleep Quality Index(PSQI)was used to evaluate clinical efficacy.[Results]The overall efficacy of the treatment group and control group was 91.1%and 77.1%,respectively,and the difference between the two was significant(P<0.05).After treatment,the PSQI scores of each group changed significantly(P<0.05).[Conclusions]Sedative cataplasm has a good clinical efficacy on sub-healthy insomnia patients.
文摘Masterpieces of world literature, classical or contemporary, have been available in Chinese translation since the beginning of the 20th century. They outweigh by far the translations of Chinese literary works. Overseas literature has had perceptible influence on Chinese modern literature and performance arts. The following ten writers and playwrights are considered leading foreign literary masters by Chinese lovers of non-Chinese literature.
基金National Innovative Drugs 13th Five-Year Major Special Project of China(Grant No.2018ZX09301030-002)
文摘Adenine is commonly used to establish the animal models for chronic kidney injury and its renal interstitial fibrosis. As an endogenous substance, adenine-induced kidney damage has not yet been fully studied and elucidated, except for inflammatory reaction. Here we analyzed the proteomics of kidney of rats after adenine overloading using LS-MS/MS assay, and observed the role of anemoside B4(B4). The results showed that adenine could down-regulate 285 proteins and up-regulate 164 proteins in rat kidney tissue compared with the normal group. Down-regulated proteins mainly affected related pathways, such as energy metabolism, while up-regulated proteins affected inflammatory response pathways and metabolic pathways. B4 could significantly reverse the down-regulation of about 40 proteins, which were involved in mitochondria, redox processes, extracellular exosomes, acetylation and other signaling pathways. Simultaneously, B4 could inhibit the up-regulation of five proteins caused by adenine, which were involved in cell cycle, oocyte meiosis, PI3 K-Akt and other signaling pathways. Further experimental results of mRNA expression using real-time PCR assay supported the proteomic analysis. Therefore, we proposed that the damage of rat kidney caused by adenine was more complicated, not only with an inflammatory reaction, but also with extensive effects to various metabolic processes in the body. This work provided a valuable clue for comprehensive understanding of adenine-induced renal damage.
