Effects of Buddlejae Flos Granules on Inflammatory Factors TGF-β1,NF-κB,IL-10 and IL-12 in Lacrimal Gland Cells of Castrated Male Rabbits

Objective To observe the effects of Buddlejae Flos (Mi Meng Hua,密蒙花) granules on the expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland cells in castrated male rabbits. Methods A total of 30 New Zealand male white rabbits were randomly divided into 5 groups. Group A: blank group;group B: model group;group C: Buddlejae Flos (Mi Meng Hua,密蒙花) granules group;group D: placebo group;group E: testosterone group. Except for the group A, all rabbits were treated with bilateral testicular and epididymis resection before intervention. Rabbits in group C were administered with Buddlejae Flos (Mi Meng Hua,密蒙花) granules (100 mg/kg), 3 times per day;Rabbits in group D were administered with normal saline, 3 times per day. Rabbits in group E were injected with testosterone propionate (0.5 mL/kg) in the thigh muscle, every 3 days. Schirmer I test (SIT) and break-up time (BUT) were measured on 1st day before modeling and 30th day after modeling. After 30 days of intervention, all rabbits were sacrificed. The removal lacrimal gland was stained in immunohistochemica staining. The expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland tissue of each group was detected, and the structure of lacrimal gland was observed. Results The results of SIT and BUT showed that there was significant difference between group B and D compared with group A (P < 0.05). There were no significant differences between group C and E (P > 0.05). There were no significant differences in the comparison of inflammatory factors TGF-β1, NF-κB, IL-10 and IL-12 between group B and D (P > 0.05). There were significant differences between group B and D compared with other groups (P < 0.01). There was no significant difference between group A, C and E (P > 0.05). Conclusions Buddlejae Flos (Mi Meng Hua,密蒙花) granules have an inhibitory effect on the expression of TGF-β1, NF-κB, IL-10 and IL-12 in castrated male rabbit lacrimal gland cells, and alleviate the inflammation of lacrimal gland tissue by inhibiting the expression of TGF-β1, NF-κB, IL-10 and IL-12 in lacrimal gland tissue, so as to treat dry eye.

Effects of Lycii Fructus and Salviae Miltiorrhizae on the Syndrome of Deficiency with Blood Stasis in RCS(rdy-/-, p-/-) Rats with Retinitis Pigmentosa: An Intervention Study

Objective To investigate the effects of Lycii Fructus(LF,Gou Qi Zi,枸杞子)and Salviae Miltiorrhizae Radix Ex Rhizoma(SM,Dan Shen,丹参)on the syndrome of deficiency with blood stasis in the RCS(rdy-/-,p-/-)rats with retinitis pigmentosa(RP).Methods A total of 32 RCS(rdy-/-,p-/-)rats were divided into 4 groups(equal amounts of female and male rats in each group):model group treated with 0.9%normal saline,LF group treated with LF formula granules,SM group treated with SM formula granules,and LF and SM(L·S)group treated with LF and SM formula granules.Eight RCS(rdy+/+,p+/+)rats(4 males and 4 females)were treated with 0.9%normal saline to serve as blank group.The contents of E2,PG,P-Selectin,plasma viscosity,whole blood relative index of the high shear rate and fibrinogen content in plasma,and the content of cAMP and cGMP in retinal homogenate were detected.The retina was evaluated by hematoxylin-eosin staining.Results The contents of E2,PG,P-Selectin,plasma viscosity,whole blood relative index of the high shear rate,and fibrinogen content in the plasma of L·S group significantly differed from those of model group(P<0.01),but were similar to those of blank group.The contents of cAMP and cGMP in the retinal homogenate of L·S group significantly differed from those in model group(P<0.01)but were similar to those in blank group(P>0.05).Conclusions LF and SM can effectively treat retinitis pigmentosa by ameliorating the syndrome of deficiency with blood stasis.

密蒙花颗粒剂对去势雄兔泪腺细胞凋亡因子Bax、Caspase-3、Fas和FasL的影响

目的观察密蒙花颗粒剂对去势雄兔泪腺细胞Bax、Caspase-3、Fas和FasL表达的影响:方法30只新西兰长耳白兔随机平均分为5组,分别为:A组:空白组,不灌胃;B组:模型组,不灌胃;C组:密蒙花颗粒剂组,灌胃药物为密蒙花颗粒剂;D组:安慰剂组,灌胃药物为生理盐水;E组:睾酮组,在兔大腿肌肉处注射丙酸睾酮注射液,,除A组外,所有兔子在干预前的造模方式均为双侧睾丸及附睾切除术,并在造模前1天和造模后第30天测定SIT和BUT。干预30天后,将所有兔子处死,摘取泪腺组织进行伊红染色观察泪腺组织结构,免疫组化法检测各组泪腺组织中Bax、Caspase-3、Fas和FasL表达结果HE染色显示:密蒙花颗粒剂组兔子泪腺组织排列整齐,细胞结构清晰,有少量炎症细胞浸润和细胞凋亡,Bax sCaspase-3、Fas和FasL少量表达于细胞膜和细胞浆中免疫组化检测显示密蒙花颗粒剂组Bax、Caspase-3、Fas和FasL平均光密度均较模型组减少(P<0.01).结论密蒙花颗粒剂对去势雄兔泪腺细胞Bax、Caspase-3、Fas和FasL表达具有抑制作用;密蒙花颗粒剂通过抑制泪腺组织中Bax xCaspase-3、Fas和FasL的表达减轻泪腺组织的凋亡,达到治疗干眼的目的。

密蒙花滴眼液对去势兔干眼模型泪腺细胞凋亡相关因子Caspase-3、Caspase-8的干预作用

目的通过建立去势兔干眼模型,观察密蒙花滴眼液对其泪腺细胞凋亡相关因子Caspase-3、Caspase-8的干预作用。方法将35只新西兰白兔随机分为正常组(A组)、模型组(B组)、0.3、0.5、1.0 mg/mL、1.5、3.0 mg/mL密蒙花滴眼液(C、D、E、F、G组)。A、B组不予处理,C、D、E、F、G组分别予以相应密蒙花滴眼液局部滴眼,3次/日。疗程为4周。造模前、用药前及用药后检测各组基础泪液分泌试验(Schirmer′s test I,SIT)水平,免疫组化检测各组泪腺组织中Caspase-3、Caspase-8的表达情况,Real-time PCR法检测各组泪腺组织中Caspase-3、Caspase-8 mRNA的相对表达情况。结果与A比较,用药前及用药后B组SIT值降低(P<0.01);与B组比较,各治疗组用药后SIT值升高(P<0.01);各治疗组用药后与本组用药前比较,SIT值升高(P<0.01)。与A组比较,B组Caspase-3、Caspase-8表达及mRNA相对表达量升高(P<0.01)。与B组比较,D、F、G组Caspase-3及C、D、E、F、G组Caspase-8表达降低(P<0.01);C、D、E、F、G组Caspase-3及D、E、F、G组Caspase-8 mRNA相对表达量降低(P<0.01)。与C组比较,Caspase-3表达降低(P<0.05);D、E、F、G组Caspase-8 mRNA相对表达量降低(P<0.01)。除D与G组比较外,其他各浓度组间Caspase-3 mRNA相对表达量比较均有统计学意义(P<0.01)。结论密蒙花滴眼液可以通过下调去势兔干眼模型泪腺细胞凋亡因子Caspase-3和Caspase-8的表达,达到维持泪腺基础分泌量和保护泪腺的作用。