A study of the fragments of DNA irradiated with MeV ions is important for the understanding of the DNA damage mechanism and the subsequent biological effects (induced by heavy ions). In this experiment, the products o...A study of the fragments of DNA irradiated with MeV ions is important for the understanding of the DNA damage mechanism and the subsequent biological effects (induced by heavy ions). In this experiment, the products of calf thymus DNA (CT DNA) irradiated with MeV fluorine ions were analyzed using agarose gel electrophoresis, modified time-of-flight mass spectrometer (MALDI-TOF), and high-performance liquid chromatography (HPLC). The results showed that the molecular mass of the fragments were concentrated around 831 bp with agarose gel elec- trophoresis, there was no observable product in the range of 1,000 ? 30,000 (m/q) using MALDI-TOF, and small bio- molecules were separated from the products. The results of this study indicated that the strand breaks of calf thymus DNA induced by MeV fluorine ions were nonrandom.展开更多
[Objective] To provide a reference for scientific and rational utilization of forage silage. [Method] The number and species of microorganisms in forage were analyzed, and the characteristics of 11 strains of lactic a...[Objective] To provide a reference for scientific and rational utilization of forage silage. [Method] The number and species of microorganisms in forage were analyzed, and the characteristics of 11 strains of lactic acid bacteria （LAB） were studied. [ Result] All the isolates were Gram-positive, catalase negative and aeroanaerobic. The isolated strains of homofermentation accounted for 78%. The isolates were divided into three groups, Lactobacillus, Leuconostocaceae and Lactococcus, according to their morphological and biochemical characters. [ Conclusion] The microorganisms in forage in northwest China were isolated and identified, which provides a scientific basis for utilization of forage silage.展开更多
文摘A study of the fragments of DNA irradiated with MeV ions is important for the understanding of the DNA damage mechanism and the subsequent biological effects (induced by heavy ions). In this experiment, the products of calf thymus DNA (CT DNA) irradiated with MeV fluorine ions were analyzed using agarose gel electrophoresis, modified time-of-flight mass spectrometer (MALDI-TOF), and high-performance liquid chromatography (HPLC). The results showed that the molecular mass of the fragments were concentrated around 831 bp with agarose gel elec- trophoresis, there was no observable product in the range of 1,000 ? 30,000 (m/q) using MALDI-TOF, and small bio- molecules were separated from the products. The results of this study indicated that the strand breaks of calf thymus DNA induced by MeV fluorine ions were nonrandom.
基金supported by the grants from Application of Nuclear Techniques in Agriculture of the Chinese Ministry of Agriculture (200803034)Natural Science Foundation of Henan Province (2007180048 and 2010B180029)
文摘[Objective] To provide a reference for scientific and rational utilization of forage silage. [Method] The number and species of microorganisms in forage were analyzed, and the characteristics of 11 strains of lactic acid bacteria （LAB） were studied. [ Result] All the isolates were Gram-positive, catalase negative and aeroanaerobic. The isolated strains of homofermentation accounted for 78%. The isolates were divided into three groups, Lactobacillus, Leuconostocaceae and Lactococcus, according to their morphological and biochemical characters. [ Conclusion] The microorganisms in forage in northwest China were isolated and identified, which provides a scientific basis for utilization of forage silage.
