Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor(ABE)-nC as9 tool that induced targeted ...Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor(ABE)-nC as9 tool that induced targeted A·T to G·C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.展开更多
Plant height is a critical trait for yield in rice(Oryza sativa),and gibberellic acid(GA)is involved in modulating rice height.Here,we identified the semi-dwarf 5(sdf5)mutant by screening a rice T-DNA insertion mutant...Plant height is a critical trait for yield in rice(Oryza sativa),and gibberellic acid(GA)is involved in modulating rice height.Here,we identified the semi-dwarf 5(sdf5)mutant by screening a rice T-DNA insertion mutant library using genome-resequencing.sdf5 contains a T-DNA insertion that causes the ectopic expression of LOC_Os05g34325,which encodes a 14-α-demethylase cytochrome P450(CYP51)protein.Levels of the active GA species GA1 and GA4 were reduced in the sdf5 mutant,and the semi-dwarf phenotype of the sdf5 mutant was rescued by exogenous GA4.In addition to its semi-dwarf height,the sdf5 mutant exhibited compact plant architecture,dark green leaves and low 1000-grain weight.Together,these results suggest that SDF5 is involved in GA biosynthesis pathway and modulates rice height and plant development.展开更多
基金supported by the National Natural Science Foundation of China (Grant No. 31501239)the Genetically Modified Breeding Major Projects (Grant No. 2016ZX08010-002-008)
文摘Precise base editing is highly desired in plant functional genomic research and crop molecular breeding. In this study, we constructed a rice-codon optimized adenine base editor(ABE)-nC as9 tool that induced targeted A·T to G·C point mutation of a key single nucleotide polymorphism site in an important agricultural gene. Combined with the modified single-guide RNA variant, our plant ABE tool can efficiently achieve adenine base editing in the rice genome.
基金supported by the Natural Science Foundation of Anhui Province in China(Grant No.1808085MC66)the Program of Rice Genetic Breeding Key Laboratory of Anhui Province(Grant No.SDKF-2020-02)University Synergy Innovation Program of Anhui Province(Grant No.GXXT-2019-033)in China.
文摘Plant height is a critical trait for yield in rice(Oryza sativa),and gibberellic acid(GA)is involved in modulating rice height.Here,we identified the semi-dwarf 5(sdf5)mutant by screening a rice T-DNA insertion mutant library using genome-resequencing.sdf5 contains a T-DNA insertion that causes the ectopic expression of LOC_Os05g34325,which encodes a 14-α-demethylase cytochrome P450(CYP51)protein.Levels of the active GA species GA1 and GA4 were reduced in the sdf5 mutant,and the semi-dwarf phenotype of the sdf5 mutant was rescued by exogenous GA4.In addition to its semi-dwarf height,the sdf5 mutant exhibited compact plant architecture,dark green leaves and low 1000-grain weight.Together,these results suggest that SDF5 is involved in GA biosynthesis pathway and modulates rice height and plant development.
