Biomanufacturing(BM)is a multidisciplinary area incorporating the characteristics of living organisms and engineering principles to create valuable products for various sectors,including medicine,energy,and the enviro...Biomanufacturing(BM)is a multidisciplinary area incorporating the characteristics of living organisms and engineering principles to create valuable products for various sectors,including medicine,energy,and the environment.BM has undergone a remarkable transformation in the last two decades,entering the era of BM4.0 and becoming a pivotal driver of the sustainable revolution.Notably,Japan has made significant advances in BM,contributing to its development through the creation of innovative materials,advanced processes,and interdisciplinary applications.However,because of certain development policies,this research has not been widely recognized on an international level.This paper provides a comprehensive summary of the research progress made by renowned Japanese laboratories and researchers in biomedical materials,bio-three-dimensional(3D)printing,and biomedical applications in the last five years.Their unique contributions are introduced and analyzed,illuminating the distinctive approaches and breakthroughs within each domain.Additionally,this review highlights the current challenges and prospects of BM.The viewpoints presented in this paper are intended to serve as a valuable reference for scholars studying BM in Japan.展开更多
After decades of research and development, the WSR-88 D(NEXRAD) network in the United States was upgraded with dual-polarization capability, providing polarimetric radar data(PRD) that have the potential to improve we...After decades of research and development, the WSR-88 D(NEXRAD) network in the United States was upgraded with dual-polarization capability, providing polarimetric radar data(PRD) that have the potential to improve weather observations,quantification, forecasting, and warnings. The weather radar networks in China and other countries are also being upgraded with dual-polarization capability. Now, with radar polarimetry technology having matured, and PRD available both nationally and globally, it is important to understand the current status and future challenges and opportunities. The potential impact of PRD has been limited by their oftentimes subjective and empirical use. More importantly, the community has not begun to regularly derive from PRD the state parameters, such as water mixing ratios and number concentrations, used in numerical weather prediction(NWP) models.In this review, we summarize the current status of weather radar polarimetry, discuss the issues and limitations of PRD usage, and explore potential approaches to more efficiently use PRD for quantitative precipitation estimation and forecasting based on statistical retrieval with physical constraints where prior information is used and observation error is included. This approach aligns the observation-based retrievals favored by the radar meteorology community with the model-based analysis of the NWP community. We also examine the challenges and opportunities of polarimetric phased array radar research and development for future weather observation.展开更多
Background: Minimal hepatic encephalopathy(MHE) is an early and reversible form of hepatic encephalopathy. The documentations on the treatment with probiotics are inconsistent. The present meta-analysis was to verify ...Background: Minimal hepatic encephalopathy(MHE) is an early and reversible form of hepatic encephalopathy. The documentations on the treatment with probiotics are inconsistent. The present meta-analysis was to verify the role of probiotics in the treatment of cirrhotic patients with MHE.Data sources: Seven electronic databases were searched for relevant randomized controlled trials(RCTs)published until July 2015. The effects of probiotics on serum ammonia, endotoxin, and MHE were evaluated.Results: A total of 14 RCTs(combined n = 1132) were included in the meta-analysis. When probiotics were compared to placebo or no treatment, probiotics were more likely to reduce values in the number connection test(NCT; week 4: MD =-30.25, 95% CI:-49.85 to-10.66), improve MHE(week 4: OR = 0.18,95% CI: 0.07 to 0.47; week 12: OR = 0.15, 95% CI: 0.07 to 0.32), and prevent overt HE progression(week4: OR = 0.22, 95% CI: 0.07 to 0.67) in patients with liver cirrhosis. When probiotics was compared to lactulose, probiotics tended to reduce serum ammonia levels(week 4: MD =-0.33 μmol/L, 95% CI:-5.39 to 4.74; week 8: MD = 6.22 μmol/L, 95% CI:-24.04 to 36.48), decrease NCT(week 8: MD = 3.93, 95% CI:-0.72 to 8.58), improve MHE(week 4: OR = 0.93, 95% CI: 0.45 to 1.91; week 12: OR = 0.73, 95% CI: 0.35 to 1.51) and prevent the development of overt HE(week 4: OR = 0.96, 95% CI: 0.17 to 5.44; week 12:OR = 2.7, 95% CI: 0.50 to 14.64) in patients with liver cirrhosis. However, lactulose appears to be more effective in reducing NCT values as compared to probiotics(week 4: MD = 6.7, 95% CI: 0.58 to 12.82).Conclusion: Probiotics can decrease serum ammonia and endotoxin levels, improve MHE, and prevent overt HE development in patients with liver cirrhosis.展开更多
AIM To investigate the mechanism of chaperone-mediated autophagy(CMA)-induced resistance to irradiationtriggered apoptosis through regulation of the p53 protein in hepatocellular carcinoma(HCC). METHODS Firstly, we de...AIM To investigate the mechanism of chaperone-mediated autophagy(CMA)-induced resistance to irradiationtriggered apoptosis through regulation of the p53 protein in hepatocellular carcinoma(HCC). METHODS Firstly, we detected expression of lysosome-associated membrane protein 2a(Lamp-2a), which is the key protein of CMA, by western blot in Hep G2 and SMMC7721 cells after irradiation. We further used sh RNA Lamp-2a HCC cells to verify the radioresistance induced by CMA. Next, we detected the HMGB1 and p53 expression after irradiation by western blot, and we further used RNA interference and ethyl pyruvate(EP), as a HMGB1 inhibitor, to observe changes of p53 expression. Finally, an immunoprecipitation assay was conducted to explore the interaction between Lamp-2a and HMGB1, and the data were analyzed. RESULTS We found the expression of Lamp-2a was increased on irradiation while apoptosis decreased in Hep G2 and SMMC7721 cells. The apoptosis was increased markedly in the sh RNA Lamp-2a Hep G2 and SMMC7721 cells as detected by western blot and colony formation assay. Next, we found p53 expression was gradually reduced on irradiation but obviously increased in sh RNA Lamp-2a cells. Furthermore, p53 increased the cell apoptosis on irradiation in Hep3B(p53-/-) cells. Finally, p53 levels were regulated by HMGB1 as measured through RNA interference and the EP treatment. HMGB1 was able to combine with Lamp-2a as seen by immunoprecipitation assay and was degraded via the CMA pathway. The decreased HMGB1 inhibited p53 expression induced by irradiation and further reduced the apoptosis in HCC cells. CONCLUSION CMA pathway activation appears to down-regulate the susceptibility of HCC to irradiation by degrading HMGB1 with further impact on p53 expression. These findings have clinical relevance for radiotherapy of HCC.展开更多
A new classification of petroleum systems(PSs) based on reservoir qualities is proposed. We classify PSs into the following three basic types:(1) source-rock petroleum system(SPS);(2) tight-reservoir or tight petroleu...A new classification of petroleum systems(PSs) based on reservoir qualities is proposed. We classify PSs into the following three basic types:(1) source-rock petroleum system(SPS);(2) tight-reservoir or tight petroleum system(TPS);and(3) conventional-reservoir or conventional petroleum system(CPS). The CPS is a PS in which hydrocarbons accumulate in conventional reservoirs, and all the essential elements and processes are significant and indispensable. Oil and gas accumulations are geographically discrete and therefore exist as discontinuous accumulations. The TPS is a PS where hydrocarbons accumulate in tight reservoirs and the source rock, reservoir, seal, migration, and trap are also indispensable,but the traps are mostly non-anticlinal and the accumulations are primarily quasi-continuous and secondarily discontinuous. The SPS is a PS where both hydrocarbon generation and accumulation occurred in source rocks and traps and migration are unnecessary or inconsequential; the hydrocarbon distribution is extensive and continuous and has no distinct boundaries. The aforementioned three PSs can be derived from a common hydrocarbon source kitchen and are closely linked in terms of their formation and distribution. Therefore, to maximize the exploration efficiency, a comprehensive study and different strategies are needed by considering the SPS, TPS, and CPS as parts of a greater whole.展开更多
Kruppel-like factor 4（Klf4） is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis.However, its regulation during embryogenesis is still unclear. Here, we report that Tcf711, a key do...Kruppel-like factor 4（Klf4） is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis.However, its regulation during embryogenesis is still unclear. Here, we report that Tcf711, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf711. Moreover, the dominant negative form of Tcf711（dnTcf711）, which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent.Taken together, our results demonstrate that Tcf711 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.展开更多
The NAC transcription factor family is plant-specific with various biological functions.However,there are few studies on the NAC gene involving coniferous species.Bioinformatics research and expression analysis of NAC...The NAC transcription factor family is plant-specific with various biological functions.However,there are few studies on the NAC gene involving coniferous species.Bioinformatics research and expression analysis of NAC genes in Larix olgensis can be used to analyse the function of the NAC gene in the future.Screening of excellent genetic materials and molecular breeding have been utilized to cultivate high-quality,stress-resistant larches.According to the transcriptome data for L.olgensis,the genes Uni-gene81490 and Unigene70699 with complete ORFs(open reading frames)were obtained by conserved domain analy-sis and named LoNAC1 and LoNAC2,respectively.The cDNAs of LoNAC1 and LoNAC2 were 1971 bp and 1095 bp in length,encoding 656 and 364 amino acids,respectively.The molecular weights of the proteins encoded by the two genes were predicted to be 72.61 kDa and 41.13 kDa,and subcellular localization analysis indicated that the proteins were concentrated in the nucleus.The results of real-time quantitative PCR analysis showed that at different growth stages and in different tissues of L.olgensis,the relative expression levels of the two NAC genes were highest in the stem,and the expression differences were more obvious in non-lignified tissues.After drought,salt and alkali stress and hormone treatment,expression was induced to different degrees.The expression levels of LoNAC1 and LoNAC2 in semi-lignified L.olgensis were higher than in the other two periods(non-lignified and lignified),and expression levels significantly increased under drought and salt stress.Relative expression levels changed under hormone treatment.It is speculated that these two genes may not only be related to drought and salt stress and secondary growth but may also be induced by hormones such as abscisic acid.Overall,LoNAC1 and LoNAC2 are genetic materials that can be used for molecular breeding of larch.展开更多
The appropriate regulation of band structure is an effective strategy in constructing efficient photocatalytic systems.Present photocatalytic system mainly employs powder photocatalysts,which makes their recovery reli...The appropriate regulation of band structure is an effective strategy in constructing efficient photocatalytic systems.Present photocatalytic system mainly employs powder photocatalysts,which makes their recovery reliant on expensive separation processes and severely limits their industrial application.Herein,we constructed a novel CdS/Ni_(3)S_(2)heterostructure using free-standing and flexible nickel fiber paper as the matrix.The regulated energy band structure achieves effective electron–hole separation.The as-synthesized flexible photocatalyst exhibits considerable photocatalytic activity toward the H_(2)evolution reaction under visible-light irradiation,with an H_(2)production rate of5.63μmol·cm^(-2)·h^(-1)(14.1 mmol·g^(-1)cat·h^(-1)according to the catalyst loading content).Additionally,the otherwisewasted excited holes simultaneously drive organic transformations to yield value-added organic products,thus markedly improving the photocatalytic H_(2)evolution rate.Such a photocatalytic system is scaled up further,where a self-supported 20 cm×25 cm sample achieves a champion H_(2)production rate of 60-80μmol·h^(-1)under practical sun irradiation.This newly developed self-supported photocatalyst produces opportunities for practical solar H2production with biomass upgrading.展开更多
Background The number of pediatric cases of infection with the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant has increased.Here,we describe the clinical characteristics of children in a te...Background The number of pediatric cases of infection with the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant has increased.Here,we describe the clinical characteristics of children in a tertiary children's medical center in Shanghai.Methods A total of 676 pediatric coronavirus disease 2019(COVID-19)cases caused by the Omicron variant who were admitted to the Shanghai Children's Medical Center from March 28 to April 30,2022 were enrolled in this single-center,prospective,observational real-world study.Patient demographics and clinical characteristics,especially COVID-19 vaccine status,were assessed.Results Children of all ages appeared susceptible to the SARS-CoV-2 Omicron variant,with no significant difference between sexes.A high SARS-CoV-2 viral load upon admission was associated with leukocytopenia,neutropenia,and thrombocytopenia(P=0.003,P=0.021,and P=0.017,respectively)but not with physical symptoms or radiographic chest abnormalities.Univariable linear regression models indicated that comorbidities(P=0.001)were associated with a longer time until viral clearance,and increasing age(P<0.001)and two doses of COVID-19 vaccine(P=0.001)were associated with a shorter time to viral clearance.Multivariable analysis revealed an independent effect of comorbidities(P<0.001)and age(P=0.003).The interaction effect between age and comorbidity showed that the negative association between age and time to virus clearance remained significant only in patients without underlying diseases(P<0.001).Conclusion This study describes the clinical characteristics of children infected with the Omicron variant of SARS-CoV-2 and calls for additional studies to evaluate the effectiveness and safety of vaccination against COVID-19 in children.展开更多
Background Chemotherapy and hematopoietic stem cell transplantation(HSCT)can damage the immune system,and may result in a loss of protection from infectious diseases.This study aimed to evaluate the impact of these tr...Background Chemotherapy and hematopoietic stem cell transplantation(HSCT)can damage the immune system,and may result in a loss of protection from infectious diseases.This study aimed to evaluate the impact of these treatments on the decrease in antibody titers of the measles,mumps,and rubella(MMR)vaccine and seroconversion post-revaccination of MMR.Methods After completion of treatment for primary diseases,participants received an MMR revaccination.Antibody titers for MMR before revaccination were analyzed for all 110 children.After revaccination,68 participants received a follow-up evaluation of antibody titer and adverse reaction.Results Multivariable analysis showed that therapeutic schedules were the only factor correlated with lack of antibody titers for measles after completing treatment(P=0.008),while for mumps and rubella,no statistically significant difference was observed.Importantly,our study clearly demonstrated positive seroconversion rates for measles(97.5%),mumps(81.0%),and rubella(93.2%),with antibody levels rising across the board and peaking at around 6 months following revaccination.However,6 months after revaccination,a downtrend of antibody titer levels was observed,which is comparatively earlier than the waning immunity observed in healthy children.Furthermore,we found MMR revaccination to be safe,with only a single adverse reaction(local pain at the injection site)reported.Conclusions MMR revaccination is immunogenic for the population.We suggest periodic monitoring of antibody titers,in addition to a booster vaccination,although the optimal timing of booster vaccination remains to be investigated further.展开更多
Tropical cyclones(TCs)forecasts from seven global models in 2013 were assessed to study the current capability of track and intensity forecast guidance over the western North Pacific.Analysis of along-and cross-track ...Tropical cyclones(TCs)forecasts from seven global models in 2013 were assessed to study the current capability of track and intensity forecast guidance over the western North Pacific.Analysis of along-and cross-track error revealed stepped decreases in the values of each quantile at each lead time level by showing the annual track error distribution from 2010 to 2013,particularly in the ECMWF-IFS,NCEP-GFS and UKMO-Met UM models.The TC propagation direction was much easier to handle for most of the global models;however,the propagation speed seemed to be more closely linked to the inner-core dynamics and thus processes that take place at smaller spatial scales.A new model evaluation tool,‘track error rose’,was used to analyze the models’systematic error in the track forecast using the same concepts as the‘wind rose’.The results showed that as the lead time increased,most of the global models forecast a TC moving speed that was slower than observations and the largest track error often appeared around the rear direction of the observation position.Another new model evaluation tool,the Taylor diagram,was used to evaluate the intensity predictions from the global models.A Taylor diagram provides a way of plotting standard deviation,centered root mean square,and the correlation coefficient on a two-dimensional graph,indicating how closely a predicted TC intensity matches observations.This made it easy to distinguish the intensity forecast performance of the seven global models and determine which models were in relatively good agreement with observations.Furthermore,it also provided a statistical measure of the correlation between modeled and observed TC intensity,offering a practical way of assessing and summarizing model capability.展开更多
Forecasts of tropical cyclone(TC)tracks from six global models during 2010 and 2012 were assessed to study the current capability of track forecast guidance over the western North Pacific.To measure the performance of...Forecasts of tropical cyclone(TC)tracks from six global models during 2010 and 2012 were assessed to study the current capability of track forecast guidance over the western North Pacific.To measure the performance of the global model forecasts,a series of statistical evaluations of track forecasts up to 120 h were carried out,including the mean,median,percentile distribution,regional distribution,relative position,correlation analysis,and binned analysis.Results showed that certain improvements have been made for the six global models in their prediction accuracy and stability in the past three years.Remarkably,stepped decreases in the values of each quantile were found at all lead time levels from 2010 to 2012 for NCEP-GFS.An analysis of the regional distribution of position errors showed that a high-latitude region,low-latitude region(which covers mostly the TC genesis region)and the South China Sea are the three main areas within which large errors tend to concentrate.The majority of the models show their own respective characteristics of systematic bias at each lead time,as established through the relative position analysis results.Only the results of NCEP-GFS and CMA-T639 did not show any obvious systematic bias in the three-year study period.Binned analyses indicated that the prediction accuracy and stability of most of the models were better for strong TCs than for weak TCs at short lead time levels.It was also found that the models tend to perform better for initially large TCs,or for those with weak vertical wind shear at lead times shorter than 48 h.The results demonstrate the heavy reliance of forecast errors upon the initial characteristics of a TC or its environmental conditions.展开更多
Phosphorescent carbon nanodots(CNDs)have various attractive properties and potential applications,but it remains a formidable challenge to achieve large-scale phosphorescent CNDs limited by current methods.Herein,a la...Phosphorescent carbon nanodots(CNDs)have various attractive properties and potential applications,but it remains a formidable challenge to achieve large-scale phosphorescent CNDs limited by current methods.Herein,a large-scale synthesis method for phosphorescent CNDs has been demonstrated via precursors’self-exothermic reaction at room temperature.The as-prepared CNDs show fluorescence and phosphorescence property,which are comparable with that synthesized by solvothermal and microwave method.Experimental and computational studies indicate that exotic atom doped sp^(2) hybridized carbon core works as an emissive center,which facilities the intersystem crossing from singlet state to triplet state.The CNDs show phosphorescence with tunable lifetimes from 193 ms to 1.13 s at different temperatures.The demonstration of large-scale synthesis of phosphorescent CNDs at room temperature opens up a new window for room temperature fabrication phosphorescent CNDs.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.52105072).
文摘Biomanufacturing(BM)is a multidisciplinary area incorporating the characteristics of living organisms and engineering principles to create valuable products for various sectors,including medicine,energy,and the environment.BM has undergone a remarkable transformation in the last two decades,entering the era of BM4.0 and becoming a pivotal driver of the sustainable revolution.Notably,Japan has made significant advances in BM,contributing to its development through the creation of innovative materials,advanced processes,and interdisciplinary applications.However,because of certain development policies,this research has not been widely recognized on an international level.This paper provides a comprehensive summary of the research progress made by renowned Japanese laboratories and researchers in biomedical materials,bio-three-dimensional(3D)printing,and biomedical applications in the last five years.Their unique contributions are introduced and analyzed,illuminating the distinctive approaches and breakthroughs within each domain.Additionally,this review highlights the current challenges and prospects of BM.The viewpoints presented in this paper are intended to serve as a valuable reference for scholars studying BM in Japan.
基金supported by the NOAA (Grant Nos. NA16AOR4320115 and NA11OAR4320072)NSF (Grant No. AGS-1341878)
文摘After decades of research and development, the WSR-88 D(NEXRAD) network in the United States was upgraded with dual-polarization capability, providing polarimetric radar data(PRD) that have the potential to improve weather observations,quantification, forecasting, and warnings. The weather radar networks in China and other countries are also being upgraded with dual-polarization capability. Now, with radar polarimetry technology having matured, and PRD available both nationally and globally, it is important to understand the current status and future challenges and opportunities. The potential impact of PRD has been limited by their oftentimes subjective and empirical use. More importantly, the community has not begun to regularly derive from PRD the state parameters, such as water mixing ratios and number concentrations, used in numerical weather prediction(NWP) models.In this review, we summarize the current status of weather radar polarimetry, discuss the issues and limitations of PRD usage, and explore potential approaches to more efficiently use PRD for quantitative precipitation estimation and forecasting based on statistical retrieval with physical constraints where prior information is used and observation error is included. This approach aligns the observation-based retrievals favored by the radar meteorology community with the model-based analysis of the NWP community. We also examine the challenges and opportunities of polarimetric phased array radar research and development for future weather observation.
基金supported by grants from the Mega-Project for National Science and Technology Development under the"11th and 12th Five-Year Plan of China"(2013ZX10004901,2013ZX10004904and 2014ZX10004008)
文摘Background: Minimal hepatic encephalopathy(MHE) is an early and reversible form of hepatic encephalopathy. The documentations on the treatment with probiotics are inconsistent. The present meta-analysis was to verify the role of probiotics in the treatment of cirrhotic patients with MHE.Data sources: Seven electronic databases were searched for relevant randomized controlled trials(RCTs)published until July 2015. The effects of probiotics on serum ammonia, endotoxin, and MHE were evaluated.Results: A total of 14 RCTs(combined n = 1132) were included in the meta-analysis. When probiotics were compared to placebo or no treatment, probiotics were more likely to reduce values in the number connection test(NCT; week 4: MD =-30.25, 95% CI:-49.85 to-10.66), improve MHE(week 4: OR = 0.18,95% CI: 0.07 to 0.47; week 12: OR = 0.15, 95% CI: 0.07 to 0.32), and prevent overt HE progression(week4: OR = 0.22, 95% CI: 0.07 to 0.67) in patients with liver cirrhosis. When probiotics was compared to lactulose, probiotics tended to reduce serum ammonia levels(week 4: MD =-0.33 μmol/L, 95% CI:-5.39 to 4.74; week 8: MD = 6.22 μmol/L, 95% CI:-24.04 to 36.48), decrease NCT(week 8: MD = 3.93, 95% CI:-0.72 to 8.58), improve MHE(week 4: OR = 0.93, 95% CI: 0.45 to 1.91; week 12: OR = 0.73, 95% CI: 0.35 to 1.51) and prevent the development of overt HE(week 4: OR = 0.96, 95% CI: 0.17 to 5.44; week 12:OR = 2.7, 95% CI: 0.50 to 14.64) in patients with liver cirrhosis. However, lactulose appears to be more effective in reducing NCT values as compared to probiotics(week 4: MD = 6.7, 95% CI: 0.58 to 12.82).Conclusion: Probiotics can decrease serum ammonia and endotoxin levels, improve MHE, and prevent overt HE development in patients with liver cirrhosis.
基金Supported by Natural Science Foundation of Hebei Province,China,No.H2016209007
文摘AIM To investigate the mechanism of chaperone-mediated autophagy(CMA)-induced resistance to irradiationtriggered apoptosis through regulation of the p53 protein in hepatocellular carcinoma(HCC). METHODS Firstly, we detected expression of lysosome-associated membrane protein 2a(Lamp-2a), which is the key protein of CMA, by western blot in Hep G2 and SMMC7721 cells after irradiation. We further used sh RNA Lamp-2a HCC cells to verify the radioresistance induced by CMA. Next, we detected the HMGB1 and p53 expression after irradiation by western blot, and we further used RNA interference and ethyl pyruvate(EP), as a HMGB1 inhibitor, to observe changes of p53 expression. Finally, an immunoprecipitation assay was conducted to explore the interaction between Lamp-2a and HMGB1, and the data were analyzed. RESULTS We found the expression of Lamp-2a was increased on irradiation while apoptosis decreased in Hep G2 and SMMC7721 cells. The apoptosis was increased markedly in the sh RNA Lamp-2a Hep G2 and SMMC7721 cells as detected by western blot and colony formation assay. Next, we found p53 expression was gradually reduced on irradiation but obviously increased in sh RNA Lamp-2a cells. Furthermore, p53 increased the cell apoptosis on irradiation in Hep3B(p53-/-) cells. Finally, p53 levels were regulated by HMGB1 as measured through RNA interference and the EP treatment. HMGB1 was able to combine with Lamp-2a as seen by immunoprecipitation assay and was degraded via the CMA pathway. The decreased HMGB1 inhibited p53 expression induced by irradiation and further reduced the apoptosis in HCC cells. CONCLUSION CMA pathway activation appears to down-regulate the susceptibility of HCC to irradiation by degrading HMGB1 with further impact on p53 expression. These findings have clinical relevance for radiotherapy of HCC.
基金supported by National Science and Technology Major Project of China (Project No. 2016ZX05050, 2011ZX05018001-004)National Natural Science Foundation Project of China (No. 41402121 and 41502132)
文摘A new classification of petroleum systems(PSs) based on reservoir qualities is proposed. We classify PSs into the following three basic types:(1) source-rock petroleum system(SPS);(2) tight-reservoir or tight petroleum system(TPS);and(3) conventional-reservoir or conventional petroleum system(CPS). The CPS is a PS in which hydrocarbons accumulate in conventional reservoirs, and all the essential elements and processes are significant and indispensable. Oil and gas accumulations are geographically discrete and therefore exist as discontinuous accumulations. The TPS is a PS where hydrocarbons accumulate in tight reservoirs and the source rock, reservoir, seal, migration, and trap are also indispensable,but the traps are mostly non-anticlinal and the accumulations are primarily quasi-continuous and secondarily discontinuous. The SPS is a PS where both hydrocarbon generation and accumulation occurred in source rocks and traps and migration are unnecessary or inconsequential; the hydrocarbon distribution is extensive and continuous and has no distinct boundaries. The aforementioned three PSs can be derived from a common hydrocarbon source kitchen and are closely linked in terms of their formation and distribution. Therefore, to maximize the exploration efficiency, a comprehensive study and different strategies are needed by considering the SPS, TPS, and CPS as parts of a greater whole.
基金supported by the Start-up Funding of Henan University of Science and Technology(13480027) to Q. C.the Key Science Foundation of Nanjing Medical University(2015NJMUZD002)+2 种基金the Natural Science Foundation of Higher Education Institutions of Jiangsu Province(16KJB-180020)Natural Science Foundation of Jiangsu Province (BK20171053)National Natural Science Funds of China (81702747) to C.L
文摘Kruppel-like factor 4（Klf4） is a zinc finger transcription factor and plays crucial roles in Xenopus embryogenesis.However, its regulation during embryogenesis is still unclear. Here, we report that Tcf711, a key downstream transducer of the Wnt signaling pathway, could promote Klf4 transcription and stimulate Klf4 promoter activity in early Xenopus embryos. Furthermore, cycloheximide treatment showed a direct effect on Klf4 transcription facilitated by Tcf711. Moreover, the dominant negative form of Tcf711（dnTcf711）, which lacks N-terminus of the β-catenin binding motif, could still activate Klf4 transcription, suggesting that this regulation is Wnt/β-catenin independent.Taken together, our results demonstrate that Tcf711 lies upstream of Klf4 to maintain its expression level during Xenopus embryogenesis.
基金This project was supported by the National Science and Technology Major Project(2018ZX08020003-001-001)the National Natural Science Foundation of China(Grant No.31700595)+1 种基金the Fundamental Research Funds for the Central Universities(2572019BA13)Heilongjiang Touyan Innovation Team Program.
文摘The NAC transcription factor family is plant-specific with various biological functions.However,there are few studies on the NAC gene involving coniferous species.Bioinformatics research and expression analysis of NAC genes in Larix olgensis can be used to analyse the function of the NAC gene in the future.Screening of excellent genetic materials and molecular breeding have been utilized to cultivate high-quality,stress-resistant larches.According to the transcriptome data for L.olgensis,the genes Uni-gene81490 and Unigene70699 with complete ORFs(open reading frames)were obtained by conserved domain analy-sis and named LoNAC1 and LoNAC2,respectively.The cDNAs of LoNAC1 and LoNAC2 were 1971 bp and 1095 bp in length,encoding 656 and 364 amino acids,respectively.The molecular weights of the proteins encoded by the two genes were predicted to be 72.61 kDa and 41.13 kDa,and subcellular localization analysis indicated that the proteins were concentrated in the nucleus.The results of real-time quantitative PCR analysis showed that at different growth stages and in different tissues of L.olgensis,the relative expression levels of the two NAC genes were highest in the stem,and the expression differences were more obvious in non-lignified tissues.After drought,salt and alkali stress and hormone treatment,expression was induced to different degrees.The expression levels of LoNAC1 and LoNAC2 in semi-lignified L.olgensis were higher than in the other two periods(non-lignified and lignified),and expression levels significantly increased under drought and salt stress.Relative expression levels changed under hormone treatment.It is speculated that these two genes may not only be related to drought and salt stress and secondary growth but may also be induced by hormones such as abscisic acid.Overall,LoNAC1 and LoNAC2 are genetic materials that can be used for molecular breeding of larch.
基金supported by the National Natural Science Foundation of China(Nos.51972147,52022037 and 52202366)Taishan Scholars Project Special Funds(No.tsqn201812083),the Innovative Team Project of Jinan(No.2021GXRC019)+1 种基金the Natural Science Foundation of Shandong Province(Nos.ZR2019YQ20,ZR2021QE011,ZR2021JQ15 and ZR2022YQ42)the King Abdullah University of Science and Technology(KAUST)。
文摘The appropriate regulation of band structure is an effective strategy in constructing efficient photocatalytic systems.Present photocatalytic system mainly employs powder photocatalysts,which makes their recovery reliant on expensive separation processes and severely limits their industrial application.Herein,we constructed a novel CdS/Ni_(3)S_(2)heterostructure using free-standing and flexible nickel fiber paper as the matrix.The regulated energy band structure achieves effective electron–hole separation.The as-synthesized flexible photocatalyst exhibits considerable photocatalytic activity toward the H_(2)evolution reaction under visible-light irradiation,with an H_(2)production rate of5.63μmol·cm^(-2)·h^(-1)(14.1 mmol·g^(-1)cat·h^(-1)according to the catalyst loading content).Additionally,the otherwisewasted excited holes simultaneously drive organic transformations to yield value-added organic products,thus markedly improving the photocatalytic H_(2)evolution rate.Such a photocatalytic system is scaled up further,where a self-supported 20 cm×25 cm sample achieves a champion H_(2)production rate of 60-80μmol·h^(-1)under practical sun irradiation.This newly developed self-supported photocatalyst produces opportunities for practical solar H2production with biomass upgrading.
基金This work was funded by the Innovative Research Team of High-Level Local Universities in Shanghai(SHSMUZDCX20212800)Science and Technology Commission Shanghai Municipality(20JC1410200,20JC1410204)+2 种基金2021 Shanghai University Teacher Training Plan-Industry University Research Practice Plan Project(EYJ26.RLO17)Shanghai Municipal Health Commission(2019SY049)Shanghai Medical Leading Talents Program(2019LJ22).
文摘Background The number of pediatric cases of infection with the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant has increased.Here,we describe the clinical characteristics of children in a tertiary children's medical center in Shanghai.Methods A total of 676 pediatric coronavirus disease 2019(COVID-19)cases caused by the Omicron variant who were admitted to the Shanghai Children's Medical Center from March 28 to April 30,2022 were enrolled in this single-center,prospective,observational real-world study.Patient demographics and clinical characteristics,especially COVID-19 vaccine status,were assessed.Results Children of all ages appeared susceptible to the SARS-CoV-2 Omicron variant,with no significant difference between sexes.A high SARS-CoV-2 viral load upon admission was associated with leukocytopenia,neutropenia,and thrombocytopenia(P=0.003,P=0.021,and P=0.017,respectively)but not with physical symptoms or radiographic chest abnormalities.Univariable linear regression models indicated that comorbidities(P=0.001)were associated with a longer time until viral clearance,and increasing age(P<0.001)and two doses of COVID-19 vaccine(P=0.001)were associated with a shorter time to viral clearance.Multivariable analysis revealed an independent effect of comorbidities(P<0.001)and age(P=0.003).The interaction effect between age and comorbidity showed that the negative association between age and time to virus clearance remained significant only in patients without underlying diseases(P<0.001).Conclusion This study describes the clinical characteristics of children infected with the Omicron variant of SARS-CoV-2 and calls for additional studies to evaluate the effectiveness and safety of vaccination against COVID-19 in children.
文摘Background Chemotherapy and hematopoietic stem cell transplantation(HSCT)can damage the immune system,and may result in a loss of protection from infectious diseases.This study aimed to evaluate the impact of these treatments on the decrease in antibody titers of the measles,mumps,and rubella(MMR)vaccine and seroconversion post-revaccination of MMR.Methods After completion of treatment for primary diseases,participants received an MMR revaccination.Antibody titers for MMR before revaccination were analyzed for all 110 children.After revaccination,68 participants received a follow-up evaluation of antibody titer and adverse reaction.Results Multivariable analysis showed that therapeutic schedules were the only factor correlated with lack of antibody titers for measles after completing treatment(P=0.008),while for mumps and rubella,no statistically significant difference was observed.Importantly,our study clearly demonstrated positive seroconversion rates for measles(97.5%),mumps(81.0%),and rubella(93.2%),with antibody levels rising across the board and peaking at around 6 months following revaccination.However,6 months after revaccination,a downtrend of antibody titer levels was observed,which is comparatively earlier than the waning immunity observed in healthy children.Furthermore,we found MMR revaccination to be safe,with only a single adverse reaction(local pain at the injection site)reported.Conclusions MMR revaccination is immunogenic for the population.We suggest periodic monitoring of antibody titers,in addition to a booster vaccination,although the optimal timing of booster vaccination remains to be investigated further.
基金supported by WMO-TLFDP,National Natural Science Foundation of China (No.41275067,No.41405060,No.41305067)ESCAP/WMO Typhoon Committee AOP 2013Science and Technology Development Project of SMB MS201403
文摘Tropical cyclones(TCs)forecasts from seven global models in 2013 were assessed to study the current capability of track and intensity forecast guidance over the western North Pacific.Analysis of along-and cross-track error revealed stepped decreases in the values of each quantile at each lead time level by showing the annual track error distribution from 2010 to 2013,particularly in the ECMWF-IFS,NCEP-GFS and UKMO-Met UM models.The TC propagation direction was much easier to handle for most of the global models;however,the propagation speed seemed to be more closely linked to the inner-core dynamics and thus processes that take place at smaller spatial scales.A new model evaluation tool,‘track error rose’,was used to analyze the models’systematic error in the track forecast using the same concepts as the‘wind rose’.The results showed that as the lead time increased,most of the global models forecast a TC moving speed that was slower than observations and the largest track error often appeared around the rear direction of the observation position.Another new model evaluation tool,the Taylor diagram,was used to evaluate the intensity predictions from the global models.A Taylor diagram provides a way of plotting standard deviation,centered root mean square,and the correlation coefficient on a two-dimensional graph,indicating how closely a predicted TC intensity matches observations.This made it easy to distinguish the intensity forecast performance of the seven global models and determine which models were in relatively good agreement with observations.Furthermore,it also provided a statistical measure of the correlation between modeled and observed TC intensity,offering a practical way of assessing and summarizing model capability.
基金supported by the Projects for Public Welfare(Meteorology)of China(GYHY201006008)National Basic Research Program of China(2009CB421505)+2 种基金the National Natural Science Foundation of China(41275067)the Science and technology Development Project of SMB(QM201202)WMO-TLFDP,the ESCAP/WMO typhoon Committee。
文摘Forecasts of tropical cyclone(TC)tracks from six global models during 2010 and 2012 were assessed to study the current capability of track forecast guidance over the western North Pacific.To measure the performance of the global model forecasts,a series of statistical evaluations of track forecasts up to 120 h were carried out,including the mean,median,percentile distribution,regional distribution,relative position,correlation analysis,and binned analysis.Results showed that certain improvements have been made for the six global models in their prediction accuracy and stability in the past three years.Remarkably,stepped decreases in the values of each quantile were found at all lead time levels from 2010 to 2012 for NCEP-GFS.An analysis of the regional distribution of position errors showed that a high-latitude region,low-latitude region(which covers mostly the TC genesis region)and the South China Sea are the three main areas within which large errors tend to concentrate.The majority of the models show their own respective characteristics of systematic bias at each lead time,as established through the relative position analysis results.Only the results of NCEP-GFS and CMA-T639 did not show any obvious systematic bias in the three-year study period.Binned analyses indicated that the prediction accuracy and stability of most of the models were better for strong TCs than for weak TCs at short lead time levels.It was also found that the models tend to perform better for initially large TCs,or for those with weak vertical wind shear at lead times shorter than 48 h.The results demonstrate the heavy reliance of forecast errors upon the initial characteristics of a TC or its environmental conditions.
基金This work was supported by the National Natural Science Foundation of China(Nos.11904326,U1804155,and U1604263)China Postdoctoral Science Foundation(Nos.2019TQ0287,and 2019M662510)+1 种基金the Chemical Dynamics Research Center(No.21688102)the Key Technology Team of the Chinese Academy of Sciences(No.GJJSTD20190002).
文摘Phosphorescent carbon nanodots(CNDs)have various attractive properties and potential applications,but it remains a formidable challenge to achieve large-scale phosphorescent CNDs limited by current methods.Herein,a large-scale synthesis method for phosphorescent CNDs has been demonstrated via precursors’self-exothermic reaction at room temperature.The as-prepared CNDs show fluorescence and phosphorescence property,which are comparable with that synthesized by solvothermal and microwave method.Experimental and computational studies indicate that exotic atom doped sp^(2) hybridized carbon core works as an emissive center,which facilities the intersystem crossing from singlet state to triplet state.The CNDs show phosphorescence with tunable lifetimes from 193 ms to 1.13 s at different temperatures.The demonstration of large-scale synthesis of phosphorescent CNDs at room temperature opens up a new window for room temperature fabrication phosphorescent CNDs.
