Analysis of differentially expressed genes in Verruca vulgaris vs.adjacent normal skin by RNA-sequencing

Introduction:Verruca vulgaris is one of the most common low-risk HPV infections and is characterized by excessive proliferation of keratinocytes.Currently,very little genetic information is available regarding verruca vulgaris in the Chinese population.This study aimed to obtain comprehensive transcript information of verruca vulgaris by RNA sequencing.Methods:High-throughput sequencing was performed on three fresh verruca vulgaris samples and adjacent normal skin on the Illumina sequencing platform.The transcriptomes were analyzed using bioinformatics and the differentially expressed genes(DEGs)were verified by immunohistochemistry.Verruca vulgaris exhibited a unique molecular signature.Results:In total,1,643 DEGs were identified in verruca vulgaris compared to normal skin.The functions of the DEGs were studies by Gene Ontology(GO)enrichment,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis,DEGs Reactome analysis,disease annotation function,and STRING protein-protein interaction(PPI)network analysis.The results revealed 595 GO terms associated with the cell cycle,signal transduction,immune system,signaling molecules,and interaction.The Reactome analysis revealed enrichment in reversible hydration of carbon dioxide and BMP signaling,while the disease annotation function revealed that the enriched DEGs are involved in keratosis disorders.The STRING PPI network showed that the edges with the highest density mainly included the 2′-5′oligoadenylate synthase(OAS)family-related proteins.Furthermore,the M-code analysis found ISG15,IRF7,and OASL were scored as significant modules and their high expression compared to the control was verified by immunohistochemistry.Conclusion:These findings contribute to the genetic information of verruca vulgaris in the Chinese population,revealing that interferon-stimulated genes may play essential roles in verruca vulgaris.

Exogenous Inoculation of Microorganisms Effect on Root Exudates and Rhizosphere Microorganism of Tobaccos

Studying the relationship between rhizosphere microorganisms and root exudates is of great significance for the interaction between rhizosphere microorganisms and plants, and the prevention and control of soil-borne diseases. This article analyzed the effects of different microorganisms on tobacco root exudates and rhizosphere microorganisms. It was found that the bacterial wilt pathogen can greatly increase acids and amines, while the probiotic B. amyloliquefaciens ZM9 can eliminate some acids and amines. The results of the study show that the root exudates of pathogenic bacteria may contain a variety of allelochemicals that cause soil-borne diseases.

A Case of Senile Vaginitis Treated by Acupuncture

The article records details of the treatment process and efect of an elderly patient with senile vaginitis,where needling,a method of Chinese Traditional Medicine(TCM)method,was applied mainly,combined with oral administration of western medicine.It then analyses this disease from prospective of TCM.In the end,it concludes that no matter traditional needling method or traditional needling method combined with moxibustion,it has achieved a satisfied treatment effect while applying needling on chosen acupuncture points,so it is worthy being further popularized.

In situ thermal-induced generation of{Ag^(0)Ag^(Ⅰ)}dimer within Co-Ag phosphonates

The thermal decomposition of Ag NO_(3)is known to produce metallic Ag,but single-atomic dispersion is hard to achieve instead of the aggregation state of nanoparticles.Herein,we develop an efficient approach to thermally generate and stabilize single Ag atoms via the coordination effect.Two desired Co-Ag phosphonates[Ag_(2)^(Ⅰ)Co_(2)^(Ⅲ)(notp H_(3))_2(NO_(3))]X[X=NO_(3)^(-)(1)or Cl O_(4)^(-)(2)]were synthesized by solid-phase grinding method or solution crystallization.Both crystal structures reveal slightly different packing arrangements of various lattice anions and identical one-dimensional(1-D)coordination chains,formed in each case by the coordination of Ag(Ⅰ)to the metalloligand Co(notp H_(3))and NO_(3)^(-) anion.The number of Ag(Ⅰ)ions connected to each NO_(3)^(-) anion reduces from 5 in bulk Ag NO_(3) to 2 in compounds 1 and 2,leading to the Ag NO_(3)component stepwise decomposition at a lower temperature(<300℃).During the thermal decomposition,the changes of supermolecular structures and Ag oxidation states were monitored by PXRD,IR and XAFS measurements.The most interesting finding is that 1 and 2 can retain chain structures and harvest Ag(0)atoms in the chain by controlling decomposition temperatures(220℃for 1 and 254℃for 2).