健脾消积颗粒提取工艺及质量标准研究

目的:优化健脾消积颗粒的提取工艺,建立其质量标准。方法:以柚皮苷和新橙皮苷的含量为主要评价指标,利用单因素考察和正交试验确定健脾消积颗粒的最佳提取工艺。将枳壳和莪术作为指标成分建立样品的薄层色谱法(TLC);测定样品的水分、粒度和溶化性;使用高效液相色谱法(HPLC)同时测定该样品中柚皮苷和新橙皮苷的含量。结果:健脾消积颗粒的最佳提取工艺为10倍量水煎煮2次,第1次2 h、第2次1 h,稠膏相对密度为1.20~1.25(70℃);枳壳和莪术的TLC图斑点清晰且阴性样品无干扰;6批样品的水分为2.80%~3.78%,粒度为1.29%~4.97%,溶化性为全部溶化,无异物、无焦屑;HPLC测定柚皮苷、新橙皮苷质量浓度分别为9.78~97.80、10.34~103.40μg·mL^(-1)时与峰面积线性良好,加样回收率分别为99.31%~100.69%(RSD=0.49%)、99.69%~100.98%(RSD=0.47%),精密度、稳定性、重复性的RSD均小于2%,每袋健脾消积颗粒样品中柚皮苷不得少于20 mg、新橙皮苷不得少于12 mg。结论:该方法重复性好、专属性强,可用于控制健脾消积颗粒的质量。

野扇花的研究进展

综述了野扇花在化学成分、质量分析方法、药理作用、栽培技术等方面的研究内容,为扩大野扇花的应用和民族医药开发提供科学依据。

星点设计-效应面法优化野扇花颗粒制备工艺及其药效学研究

目的:制备野扇花颗粒,并对其制备工艺进行优化,同时完成制剂初步药效学研究。方法:以料液比、提取次数、提取时间为主要考察因素,以总生物碱含量为评价指标,采用星点设计(CCD)-效应面法优选野扇花颗粒制备工艺,同时观察制剂对小鼠应激性胃溃疡、小鼠热板痛阈、醋酸引起小鼠扭体反应的影响。结果:野扇花颗粒的最优制备工艺料液比为1∶12,提取2次,每次120 min,制剂可显著降低应激性胃溃疡小鼠溃疡点数,可显著延长小鼠热刺激性疼痛的潜伏期,可显著降低醋酸引起的小鼠扭体次数。结论:通过CCD效应面法优选得到的野扇花颗粒制备工艺预测性好、合理可行,同时制剂具有抗胃溃疡、镇痛的药理作用。

瘤果紫玉盘的质量标准

目的:建立瘤果紫玉盘的质量标准,控制该药材质量。方法:参照《中国药典》方法,对10批瘤果紫玉盘药材中的水分、总灰分和浸出物进行测定;采用薄层色谱法分别以槲皮素、山奈酚和对照药材为对照,对该药材进行定性鉴别;采用高效液相色谱法建立药材中芦丁的含量测定方法。结果:瘤果紫玉盘药材的水分不得过15%,总灰分不得过9%,酸不溶性总灰分不得少于2%,80%乙醇浸出物不得少于15%,醚浸出物不得少于0.5%。TLC中槲皮素和山奈酚斑点清晰、分离度好,含量测定中芦丁的精密度、稳定性和重现性试验中RSD%均<3%,瘤果紫玉盘10个产地药材芦丁含量在0.094%～0.583%,按干燥品计算,芦丁含量不得少于0.05%。结论:建立的瘤果紫玉盘药材质量标准可有效地控制药材质量,为临床合理、有效、安全用药提供参考。

Liver metabolomics study reveals protective function of Phyllanthus urinaria against CCl_4-induced liver injury

Phyllanthus Urinaria L.(PUL) is a traditional Chinese medicine used to treat hepatic and renal disorders. However, the mechanism of its hepatoprotective action is not fully understood. In the present study, blood biochemical indexes and liver histopathological changes were used to estimate the extent of hepatic injury. GC/MS and LC/MS-based untargeted metabolomics were used in combination to characterize the potential biomarkers associated with the protective activity of PUL against CCl_4-induced liver injury in rats. PUL treatment could reverse the increase in ALT, AST and ALP induced by CCl_4 and attenuate the pathological changes in rat liver. Significant changes in liver metabolic profiling were observed in PUL-treated group compared with liver injury model group. Seventeen biomarkers related to the hepatoprotective effects of PUL against CCl_4-induced liver injury were screened out using nonparametric test and Pearson's correlation analysis(OPLS-DA). The results suggested that the potential hepatoprotective effects of PUL in attenuating CCl_4-induced hepatotoxicity could be partially attributed to regulating L-carnitine, taurocholic acid, and amino acids metabolism, which may become promising targets for treatment of liver toxicity. In conclusion, this study provides new insights into the mechanism of the hepatoprotection of Phyllanthus Urinaria.