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超声波辅助提取牛油果油工艺及产品性质研究
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作者 李颖 邱妍玲 +2 位作者 罗剑斌 邱松山 萧允艺 《粮食与油脂》 北大核心 2022年第11期55-59,共5页
以牛油果为原料,在单因素试验基础上,采用响应面法优化超声波辅助提取牛油果油工艺条件,并考察其理化性质及抗氧化能力。结果表明:最佳提取工艺条件为液料比21∶1(mL/g)、提取时间11.5 min、提取温度46℃,在此条件下牛油果油的得率为44.... 以牛油果为原料,在单因素试验基础上,采用响应面法优化超声波辅助提取牛油果油工艺条件,并考察其理化性质及抗氧化能力。结果表明:最佳提取工艺条件为液料比21∶1(mL/g)、提取时间11.5 min、提取温度46℃,在此条件下牛油果油的得率为44.48%;提取的牛油果油色泽浅绿色,透明液体,具有牛油果香味,碘值297 g/100 g,折光率1.468 3,酸价1.70 mg KOH/g,皂化值164 mg KOH/g,过氧化值0.12 mmol/kg;其具有较强的抗氧化能力,对铁离子的最大还原能力为0.501 mg/mL,对DPPH自由基最大清除率为42.41%,对羟基自由基最大清除率为81.65%,对超氧阴离子自由基最大清除率为73.34%。 展开更多
关键词 牛油果油 超声波辅助 理化性质 抗氧化能力
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荔枝酵素的发酵工艺优化及其酚类化合物的转化分析 被引量:7
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作者 周英彪 邹晓桐 +7 位作者 刘骏 邓乃铨 潘巧怡 孙杨 曾杏柳 邱松山 姜翠翠 韦明肯 《生物技术》 CAS 2021年第2期176-182,197,共8页
[目的]优化荔枝酵素的发酵工艺,并初步分析其重要功能因子酚类化合物的生物转化。[方法]以荔枝果汁为原料,总酚含量为指标,单因素及正交试验优化荔枝酵素的发酵工艺,HPLC法分析其酚类化合物的生物转化。[结果]优化后的发酵工艺:菌株组... [目的]优化荔枝酵素的发酵工艺,并初步分析其重要功能因子酚类化合物的生物转化。[方法]以荔枝果汁为原料,总酚含量为指标,单因素及正交试验优化荔枝酵素的发酵工艺,HPLC法分析其酚类化合物的生物转化。[结果]优化后的发酵工艺:菌株组合为酿酒酵母+醋酸杆菌+植物乳酸杆菌(1∶1∶1)、接种量10%、发酵温度30℃、发酵时间48 h,在此工艺下,荔枝酵素的总酚含量达5.894±0.106μg/mL,是原果汁的1.54倍;所制得的荔枝酵素中至少含有24种酚类化合物,23种为新生成,其中1种可以确定为柚皮苷(含量达0.944μg/mL)。[结论]优化发酵工艺能显著增加荔枝酵素中的总酚含量,使其功能特性提升;原果汁中酚类化合物基本被转化且伴随有新的酚类化合生成,说明荔枝酵素发酵涉及复杂的生物转化。 展开更多
关键词 荔枝酵素 发酵 酚类化合物 生物转化 高效液相色谱
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Geometrical Structures and Activities of Free Radical Scavenging Studies of Baicalein and Scutellarein
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作者 qiu song-shan JIANG Cui-Cui +1 位作者 ZHOU Ru-Jin LI Chun-Hai 《Chinese Journal of Structural Chemistry》 SCIE CAS CSCD 2020年第1期57-65,2,共10页
In order to study the free radical scavenging capacity and molecular structures of baicalein and scutellarein,the structure-activity relationship of these two molecules was analyzed.The geometric structures of the com... In order to study the free radical scavenging capacity and molecular structures of baicalein and scutellarein,the structure-activity relationship of these two molecules was analyzed.The geometric structures of the compounds were optimized by DMol3 code based on density functional theory.The theoretical parameters of the atomic charge distribution,the distribution of molecular frontier orbital,the energy difference and Fukui functions were calculated.Meantime,superoxide anion free radical and 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)methods were used to determine the scavenging capacity of the compounds.The results showed that the scavenging rate of DPPH radicals and superoxide anion radicals increased with the increase of sample concentration,and had a dose-effect relationship.The radical-scavenging activities in the order of baicalein>scutellarein>BHT were showed.The p-π conjugation of the hydroxyl oxygen atom influenced the certain ability to repelling electrons in the molecular structures of baicalein and scutellarein based on the data of the atomic charge distribution,the distribution of molecular frontier orbital,the energy difference and Fukui functions.Hydroxyl groups were more susceptible to attack and exhibit free radical scavenging activity.The oxygen atom in the phenolic hydroxyl group of the molecule structure of the samples may be the reaction active sites where the electrophilic reaction occurred.These results offer useful theoretical bases for the research and application of antioxidant activities of substances containing such molecular structures. 展开更多
关键词 density FUNCTIONAL theory BAICALEIN SCUTELLAREIN free RADICAL SCAVENGING
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