Background:Lung cancer is one of the most lethal cancers worldwide,but studies have shown that the higher the expression of programmed cell death protein 1 ligand 1(PD-L1)in non-small cell lung cancer(NSCLC),the more ...Background:Lung cancer is one of the most lethal cancers worldwide,but studies have shown that the higher the expression of programmed cell death protein 1 ligand 1(PD-L1)in non-small cell lung cancer(NSCLC),the more likely it will benefit from anti-PD-L1 immunotherapy.The purpose of our study was to collect and analyze abundant clinical samples in order to provide evidence for clinicians and patients who might consider anti-PD-L1 immunotherapy while jointly formulating treatment plans.Methods:On the one hand,we obtained cases from The Cancer Genome Atlas(TCGA)database,including 498 lung squamous cell cancer(LUSC)patients and 515 lung adenocarcinoma(LUAD)patients.We studied the lung caner driver gene in LUSC and LUAD.On the other hand,PD-L1 expression was detected in lung cancer tissues of 1,008 NSCLC patients with immunohistochemistry staining(IHC),and we studied the correlation between PD-L1 protein expression and clinicopathological characteristics.Results:PD-L1 expression was higher in LUSC than in LUAD at the mRNA level.In univariate analysis,PD-L1 expression at the protein level was higher in patients who were males,were LUSC,were smokers,had a tumor diameter>3 cm,had poor differentiation,or had stages Ⅲ-Ⅳ disease.In multivariate analysis,PD-L1 expression was higher in patients who were LUSC or in poor differentiation.Conclusion:In term of protein level,PD-L1 expression was higher in NSCLC patients who were LUSC or in poor differentiation.We recommend that PD-L1 IHC detection can be routinely performed in such populations that are likely to benefit most from PD-L1 immunotherapy.展开更多
The dengue virus(DENV) is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation ...The dengue virus(DENV) is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics of the 2014 dengue strains, the envelope(E) gene sequences from 17 viral strains isolated from 168 dengue patient serum samples were sequenced and a phylogenetic tree was reconstructed. All 17 strains were serotype Ⅰ strains, including 8genotype Ⅰ and 9 genotype V strains. Additionally, 6 genotype Ⅰ strains that were probably introduced to China from Thailand before 2009 were widely transmitted in the 2013 and 2014 epidemics, and they continued to circulate until 2015, with one affinis strain being found in Singapore. The other 2 genotype Ⅰ strains were introduced from the Malaya Peninsula in 2014. The transmission source of the 9 genotype Ⅴ strains was from Malaysia in 2014. DENVs of different serotypes and genotypes co-circulated in the 2014 dengue outbreak in Guangzhou. Moreover, not only had DENV been imported to Guangzhou, but it had also been gradually exported, as the viruses exhibited an enzootic transmission cycle in Guangzhou.展开更多
We propose a novel circuit for the fractional-order memristive neural synaptic weighting(FMNSW).The introduced circuit is different from the majority of the previous integer-order approaches and offers important advan...We propose a novel circuit for the fractional-order memristive neural synaptic weighting(FMNSW).The introduced circuit is different from the majority of the previous integer-order approaches and offers important advantages. Since the concept of memristor has been generalized from the classic integer-order memristor to the fractional-order memristor(fracmemristor), a challenging theoretical problem would be whether the fracmemristor can be employed to implement the fractional-order memristive synapses or not. In this research, characteristics of the FMNSW, realized by a pulse-based fracmemristor bridge circuit, are investigated. First, the circuit configuration of the FMNSW is explained using a pulse-based fracmemristor bridge circuit. Second, the mathematical proof of the fractional-order learning capability of the FMNSW is analyzed. Finally, experimental work and analyses of the electrical characteristics of the FMNSW are presented. Strong ability of the FMNSW in explaining the cellular mechanisms that underlie learning and memory, which is superior to the traditional integer-order memristive neural synaptic weighting, is considered a major advantage for the proposed circuit.展开更多
基金supported by the Fundamental Research Funds for the Central Universities of Central South University(Grant 2021zzts1026).
文摘Background:Lung cancer is one of the most lethal cancers worldwide,but studies have shown that the higher the expression of programmed cell death protein 1 ligand 1(PD-L1)in non-small cell lung cancer(NSCLC),the more likely it will benefit from anti-PD-L1 immunotherapy.The purpose of our study was to collect and analyze abundant clinical samples in order to provide evidence for clinicians and patients who might consider anti-PD-L1 immunotherapy while jointly formulating treatment plans.Methods:On the one hand,we obtained cases from The Cancer Genome Atlas(TCGA)database,including 498 lung squamous cell cancer(LUSC)patients and 515 lung adenocarcinoma(LUAD)patients.We studied the lung caner driver gene in LUSC and LUAD.On the other hand,PD-L1 expression was detected in lung cancer tissues of 1,008 NSCLC patients with immunohistochemistry staining(IHC),and we studied the correlation between PD-L1 protein expression and clinicopathological characteristics.Results:PD-L1 expression was higher in LUSC than in LUAD at the mRNA level.In univariate analysis,PD-L1 expression at the protein level was higher in patients who were males,were LUSC,were smokers,had a tumor diameter>3 cm,had poor differentiation,or had stages Ⅲ-Ⅳ disease.In multivariate analysis,PD-L1 expression was higher in patients who were LUSC or in poor differentiation.Conclusion:In term of protein level,PD-L1 expression was higher in NSCLC patients who were LUSC or in poor differentiation.We recommend that PD-L1 IHC detection can be routinely performed in such populations that are likely to benefit most from PD-L1 immunotherapy.
基金supported by the Guangdong Natural Science Foundation (No. S2012030006598)the Science & Technology Planning Project of Guangdong Province of China (No. 2013A020229007)the Innovative Program of the State Key Laboratory of Virology (No. 2016KF001)
文摘The dengue virus(DENV) is a vital global public health issue. The 2014 dengue epidemic in Guangzhou, China, caused approximately 40,000 cases of infection and five deaths. We carried out a comprehensive investigation aimed at identifying the transmission sources in this dengue epidemic. To analyze the phylogenetics of the 2014 dengue strains, the envelope(E) gene sequences from 17 viral strains isolated from 168 dengue patient serum samples were sequenced and a phylogenetic tree was reconstructed. All 17 strains were serotype Ⅰ strains, including 8genotype Ⅰ and 9 genotype V strains. Additionally, 6 genotype Ⅰ strains that were probably introduced to China from Thailand before 2009 were widely transmitted in the 2013 and 2014 epidemics, and they continued to circulate until 2015, with one affinis strain being found in Singapore. The other 2 genotype Ⅰ strains were introduced from the Malaya Peninsula in 2014. The transmission source of the 9 genotype Ⅴ strains was from Malaysia in 2014. DENVs of different serotypes and genotypes co-circulated in the 2014 dengue outbreak in Guangzhou. Moreover, not only had DENV been imported to Guangzhou, but it had also been gradually exported, as the viruses exhibited an enzootic transmission cycle in Guangzhou.
基金Project supported by the National Key Research and Development Program of China (No. 2018YFC0830300)the National Natural Science Foundation of China (No. 61571312)。
文摘We propose a novel circuit for the fractional-order memristive neural synaptic weighting(FMNSW).The introduced circuit is different from the majority of the previous integer-order approaches and offers important advantages. Since the concept of memristor has been generalized from the classic integer-order memristor to the fractional-order memristor(fracmemristor), a challenging theoretical problem would be whether the fracmemristor can be employed to implement the fractional-order memristive synapses or not. In this research, characteristics of the FMNSW, realized by a pulse-based fracmemristor bridge circuit, are investigated. First, the circuit configuration of the FMNSW is explained using a pulse-based fracmemristor bridge circuit. Second, the mathematical proof of the fractional-order learning capability of the FMNSW is analyzed. Finally, experimental work and analyses of the electrical characteristics of the FMNSW are presented. Strong ability of the FMNSW in explaining the cellular mechanisms that underlie learning and memory, which is superior to the traditional integer-order memristive neural synaptic weighting, is considered a major advantage for the proposed circuit.
