A double mutant with streaked leaf and abnormal floret was found and temporarily named streaked leaf and floral organ number mutant （st-fon）. For this mutant, besides white streak appeared on culm, leaves and panicl...A double mutant with streaked leaf and abnormal floret was found and temporarily named streaked leaf and floral organ number mutant （st-fon）. For this mutant, besides white streak appeared on culm, leaves and panicles, the number of floral organs increased and florets cracked. The extreme phenotype was that several small florets grew from one floret or branch rachis in small florets extended and developed into panicles. By using transmission electron microscope to observe the ultrastructure of white histocytes of leaves at the seedling stage, the white tissues which showed abnormal plastids, lamellas and thylakoids could not develop into normal chloroplast, and the development of chloroplast was blocked at the early growth stage of plastid. Scanning electron microscope and paraffin section were also used to observe the development of floral organs, and the results indicated that the development of floral meristem was out of order and unlimited, whereas in the twisty leaves, vascular bundle sheath cells grew excessively, or some bubbly cells increased. Genetic analyses carried out by means of cross and backcross with four normal-leaf-color materials revealed that the mutant is of cytoplasm inheritance.展开更多
基金supported by the Foundation Program,Innovative Team Development Plan of the Ministry of Education,China(Grant No.IRT0453)the Financial Gene Engineering Excellent Article Foundation Program of Sichuan Province,China(Grant No.2011LWJJ-005)
文摘A double mutant with streaked leaf and abnormal floret was found and temporarily named streaked leaf and floral organ number mutant （st-fon）. For this mutant, besides white streak appeared on culm, leaves and panicles, the number of floral organs increased and florets cracked. The extreme phenotype was that several small florets grew from one floret or branch rachis in small florets extended and developed into panicles. By using transmission electron microscope to observe the ultrastructure of white histocytes of leaves at the seedling stage, the white tissues which showed abnormal plastids, lamellas and thylakoids could not develop into normal chloroplast, and the development of chloroplast was blocked at the early growth stage of plastid. Scanning electron microscope and paraffin section were also used to observe the development of floral organs, and the results indicated that the development of floral meristem was out of order and unlimited, whereas in the twisty leaves, vascular bundle sheath cells grew excessively, or some bubbly cells increased. Genetic analyses carried out by means of cross and backcross with four normal-leaf-color materials revealed that the mutant is of cytoplasm inheritance.
