OBJECTIVE To study the expression level and clinical significance of HMGB1 and RAGE in cervical squamous epithelial carcinoma. METHODS Real time quantitative polymerase chain reaction （qRT-PCR） was employed to exami...OBJECTIVE To study the expression level and clinical significance of HMGB1 and RAGE in cervical squamous epithelial carcinoma. METHODS Real time quantitative polymerase chain reaction （qRT-PCR） was employed to examine the expression of HMGB1 （high mobility group box protein1）, and RAGE （receptor for advanced glycation endproducts） in 60 cervical squamous epithelial carcinomas （CSEC）, their paraneoplastic tissues （PS） and 30 normal cervix tissues （NCS）. RESULTS The expression of HMGB1 in the CSEC samples and PS was similar （P〉0.05）, but higher compared to NCS （P〈0.05）. Overexpression of HMGB1 in the CESC tissues was significantly correlated with the tumor （P〈0.05）, and the presence of metastasis （P〈0.01）, but not correlated with the tumor diameter or tumor grade.RAGE expression was not significantly different among these tissue types, and showed no significant correlation with the the tumor stage, diameter or grade. But there was a significant positive correlation between RAGE expression and CSEC metastasis. CONCLUSION The results suggest that HMGB1 may be related to the proliferation, progression and metastasis of CSEC. The relationship of HMGBI/RAGE may be of importance for CSEC metastasis. HMGB1 presents a new potential gene target for prevention and treatment of CSEC. Study of HMGBI/RAGE expression will offer an experimental foundation for understanding the pathogenesis of CSES.展开更多
Plastic mulched ridge-furrow irrigation is a useful method to improve crop productivity and decrease salt accumulation in arid saline areas.However,inappropriate irrigation and fertilizer practices may result in ecolo...Plastic mulched ridge-furrow irrigation is a useful method to improve crop productivity and decrease salt accumulation in arid saline areas.However,inappropriate irrigation and fertilizer practices may result in ecological and environmental problems.In order to improve the resource use efficiency in these areas,we investigated the effects of different irrigation amounts(400(I1),300(I2)and 200(I3)mm)and nitrogen application rates(300(F1)and 150(F2)kg N/hm^(2))on water consumption,salt variation and resource use efficiency of spring maize(Zea mays L.)in the Hetao Irrigation District(HID)of Northwest China in 2017 and 2018.Result showed that soil water contents were 0.2%-8.9%and 13.9%-18.1%lower for I2 and I3 than for I1,respectively,but that was slightly higher for F2 than for F1.Soil salt contents were 7.8%-23.5%and 48.5%-48.9%lower for I2 than for I1 and I3,but that was 1.6%-5.5%higher for F1 than for F2.Less salt leaching at the early growth stage(from sowing to six-leaf stage)and higher salt accumulation at the peak growth stage(from six-leaf to tasseling stage and from grain-filling to maturity stage)resulted in a higher soil salt content for I3 than for I1 and I2.Grain yields for I1 and I2 were significantly higher than that for I3 and irrigation water use efficiency for I2 was 14.7%-34.0%higher than that for I1.Compared with F1,F2 increased the partial factor productivity(PFP)of nitrogen fertilizer by more than 80%.PFP was not significantly different between I1F2 and I2F2,but significantly higher than those of other treatments.Considering the goal of saving water and nitrogen resources,and ensuring food security,we recommended the combination of I2F2 to ensure the sustainable development of agriculture in the HID and other similar arid saline areas.展开更多
Objective： Debulking surgery （residual disease of less than 1 cm） followed by platinum-taxane chemotherapy isthe current standard therapy for patients with epithelial ovarian cancer, primary peritoneal carcinoma an...Objective： Debulking surgery （residual disease of less than 1 cm） followed by platinum-taxane chemotherapy isthe current standard therapy for patients with epithelial ovarian cancer, primary peritoneal carcinoma and primaryfallopian cancer. But the prognosis remains poor. Several chemotherapy regimens could be selected for patientswith recrudescent cancer. However, the standard second chemotherapy regimen remains a subject of debate. Thepurpose of our study is to carry out a systematic review and network meta-analysis to compare the efficacy andsafety of different regimens for recurrent platinum-resistant ovarian cancer based on existing randomized controlledtrials （RCTs）. Methods and analysis： Medline, EMBASE, Cochrane Library and Cochrane Library databases,Science Citation Index Expanded, Conference Proceedings Citation Index-Science and ClinicalTrials.gov will besystematically searched for eligible studies. Randomized controlled trials （RCT） on chemotherapy, immune therapy,biological therapy, targeted therapy for recurrent platinum-resistant ovarian cancer with multiple outcome measureswill be included. The Cochrane Risk of Bias Tool will be used to assess the quality of included studies. The primaryoutcomes are overall survival and median survival time. The secondary outcomes include the safety and side effectsof these regimens. Direct meta-analysis and network meta-analysis （NMA） will be conducted to compare differentregimens. Conclusion： The results of this network meta-analysis will provide direct and indirect evidence oftreatments for recurrent platinum-resistant ovarian cancer, and it may provide a ranking of the chemotherapyregimens for patients and gynecological oncologists to help them select the best option.展开更多
Lysine lipoylation plays vital roles in cell metabolism and redox processes.For example,removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle.Despite the important funct...Lysine lipoylation plays vital roles in cell metabolism and redox processes.For example,removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle.Despite the important functions of lysine lipoylation,the mechanisms for the addition and removal of this modification remain largely unexplored.Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins.For example,immunoaffinity purification-mass spectrometry is one of such tools,which highly relies on antibody efficiency and purification techniques.Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity.Affinitybased labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins.Herein,we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation(eraser)activity and interaction of lysine lipoylation with the eraser at the same time.We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2.We envision that the probe will help delineate the roles of delipoylation enzyme in biology.展开更多
文摘OBJECTIVE To study the expression level and clinical significance of HMGB1 and RAGE in cervical squamous epithelial carcinoma. METHODS Real time quantitative polymerase chain reaction （qRT-PCR） was employed to examine the expression of HMGB1 （high mobility group box protein1）, and RAGE （receptor for advanced glycation endproducts） in 60 cervical squamous epithelial carcinomas （CSEC）, their paraneoplastic tissues （PS） and 30 normal cervix tissues （NCS）. RESULTS The expression of HMGB1 in the CSEC samples and PS was similar （P〉0.05）, but higher compared to NCS （P〈0.05）. Overexpression of HMGB1 in the CESC tissues was significantly correlated with the tumor （P〈0.05）, and the presence of metastasis （P〈0.01）, but not correlated with the tumor diameter or tumor grade.RAGE expression was not significantly different among these tissue types, and showed no significant correlation with the the tumor stage, diameter or grade. But there was a significant positive correlation between RAGE expression and CSEC metastasis. CONCLUSION The results suggest that HMGB1 may be related to the proliferation, progression and metastasis of CSEC. The relationship of HMGBI/RAGE may be of importance for CSEC metastasis. HMGB1 presents a new potential gene target for prevention and treatment of CSEC. Study of HMGBI/RAGE expression will offer an experimental foundation for understanding the pathogenesis of CSES.
基金This work was supported by the National Natural Science Foundation of China(51879224,51609237)the Key Research and Development Projects of Shaanxi Province,China(2019NY-190).
文摘Plastic mulched ridge-furrow irrigation is a useful method to improve crop productivity and decrease salt accumulation in arid saline areas.However,inappropriate irrigation and fertilizer practices may result in ecological and environmental problems.In order to improve the resource use efficiency in these areas,we investigated the effects of different irrigation amounts(400(I1),300(I2)and 200(I3)mm)and nitrogen application rates(300(F1)and 150(F2)kg N/hm^(2))on water consumption,salt variation and resource use efficiency of spring maize(Zea mays L.)in the Hetao Irrigation District(HID)of Northwest China in 2017 and 2018.Result showed that soil water contents were 0.2%-8.9%and 13.9%-18.1%lower for I2 and I3 than for I1,respectively,but that was slightly higher for F2 than for F1.Soil salt contents were 7.8%-23.5%and 48.5%-48.9%lower for I2 than for I1 and I3,but that was 1.6%-5.5%higher for F1 than for F2.Less salt leaching at the early growth stage(from sowing to six-leaf stage)and higher salt accumulation at the peak growth stage(from six-leaf to tasseling stage and from grain-filling to maturity stage)resulted in a higher soil salt content for I3 than for I1 and I2.Grain yields for I1 and I2 were significantly higher than that for I3 and irrigation water use efficiency for I2 was 14.7%-34.0%higher than that for I1.Compared with F1,F2 increased the partial factor productivity(PFP)of nitrogen fertilizer by more than 80%.PFP was not significantly different between I1F2 and I2F2,but significantly higher than those of other treatments.Considering the goal of saving water and nitrogen resources,and ensuring food security,we recommended the combination of I2F2 to ensure the sustainable development of agriculture in the HID and other similar arid saline areas.
文摘Objective： Debulking surgery （residual disease of less than 1 cm） followed by platinum-taxane chemotherapy isthe current standard therapy for patients with epithelial ovarian cancer, primary peritoneal carcinoma and primaryfallopian cancer. But the prognosis remains poor. Several chemotherapy regimens could be selected for patientswith recrudescent cancer. However, the standard second chemotherapy regimen remains a subject of debate. Thepurpose of our study is to carry out a systematic review and network meta-analysis to compare the efficacy andsafety of different regimens for recurrent platinum-resistant ovarian cancer based on existing randomized controlledtrials （RCTs）. Methods and analysis： Medline, EMBASE, Cochrane Library and Cochrane Library databases,Science Citation Index Expanded, Conference Proceedings Citation Index-Science and ClinicalTrials.gov will besystematically searched for eligible studies. Randomized controlled trials （RCT） on chemotherapy, immune therapy,biological therapy, targeted therapy for recurrent platinum-resistant ovarian cancer with multiple outcome measureswill be included. The Cochrane Risk of Bias Tool will be used to assess the quality of included studies. The primaryoutcomes are overall survival and median survival time. The secondary outcomes include the safety and side effectsof these regimens. Direct meta-analysis and network meta-analysis （NMA） will be conducted to compare differentregimens. Conclusion： The results of this network meta-analysis will provide direct and indirect evidence oftreatments for recurrent platinum-resistant ovarian cancer, and it may provide a ranking of the chemotherapyregimens for patients and gynecological oncologists to help them select the best option.
基金supported by the Science Technology and Innovation Committee of Shenzhen Municipality(Grant Nos.JCYJ20180507181654823 and JCYJ20170413141047772)the National Natural Science Foundation of China(Grant No.21778044).
文摘Lysine lipoylation plays vital roles in cell metabolism and redox processes.For example,removal of lipoylation will decrease pyruvate dehydrogenase activity and affect the citric acid cycle.Despite the important functions of lysine lipoylation,the mechanisms for the addition and removal of this modification remain largely unexplored.Very few useful chemical tools are available to study the interactions of lysine lipoylation with its regulatory delipoylation proteins.For example,immunoaffinity purification-mass spectrometry is one of such tools,which highly relies on antibody efficiency and purification techniques.Single-step activity based fluorogenic probes developed by our groups and others is also an efficient method to study the deacylation activity.Affinitybased labeling probe using photo-cross-linker is a powerful platform to study the transient and dynamic interactions of peptide ligands with the interacting proteins.Herein,we have designed and synthesized a dual-function probe KTLlip for studying enzymatic delipoylation(eraser)activity and interaction of lysine lipoylation with the eraser at the same time.We show that KTLlip can be used as a useful tool to detect delipoylation as demonstrated by its ability to fluorescently label the eraser activity of recombinant Sirt2.We envision that the probe will help delineate the roles of delipoylation enzyme in biology.
