A near-isogenic lines(NILs) -Williams and Williams82 is used to identify molecular marker linked to the resistance gene Rps1-k by RAPD. Genomic DNAs extracted from soybean leaves of the NILs were analyzed by RAPD usin...A near-isogenic lines(NILs) -Williams and Williams82 is used to identify molecular marker linked to the resistance gene Rps1-k by RAPD. Genomic DNAs extracted from soybean leaves of the NILs were analyzed by RAPD using 160 different 10-nt random primers. Some specific DNA fragments were amplified from Williams82 with 4 primer(OPF-16, OPB-05, OPD-06 and OPH-05) which contains Rps1-k. All these specific DNA fragments were not detected in Williams. The experiment with OPH-05 was repeated 3 times and the results were the same. Using primer- OPH-05 to detect other resistance cultivars with Rps1-k, almost everyone can amplify the specific DNA fragment. So it is inferred that the specific DNA fragment is probably linked to Rps1-k.展开更多
isolates of P.sojae were obtained from diseased soybean plants in 9 counties of Heilongjiang Province and 1 county of Jilin Province in Northeast of China.11 isolates were classified into 3 races;the rest that could n...isolates of P.sojae were obtained from diseased soybean plants in 9 counties of Heilongjiang Province and 1 county of Jilin Province in Northeast of China.11 isolates were classified into 3 races;the rest that could not be classified due to intermediate reactions were classified into 12 virulence types,suggesting virulence diversity of P.sojae in China.Isolates represented race 1,3 and 8.Race 1 was the most prevalent one,and races 3 and 8 were the first report in China.Our results suggest P.sojae is aboriginal in China.展开更多
To obtain the protein of duck interferon alpha and study its biological activities, the prokaryotic expression vector of DuIFN-αwas constructed and expressed in BL21 (DE3) plysS. Using PCR technique, the protein gene...To obtain the protein of duck interferon alpha and study its biological activities, the prokaryotic expression vector of DuIFN-αwas constructed and expressed in BL21 (DE3) plysS. Using PCR technique, the protein gene of DuIFN-αwas cloned from pMD-18-duIFN-αrecombinant. The gene was then inserted to pGEM-T vector and identified by restriction endonuclease analysis and sequencing. DuIFN-αwas ligated with the prokaryotic expression vector of pET30 a, then transformed into BL21 (DE3) plysS. The best inducing time and IPTG concentration for the expression of this recombinant protein was tested through the expression of the positive recombinant with different time span and different IPTG concentration. Lots of the protein of DuIFN-αwere expressed in BL21(DE3)plysS with 1 mmol·L-1 IPTG for 4 hours and its molecular weight for 34 000.展开更多
Soybean Phytophthora root rot (Phytophthora sojae) is a severe disease all over the world. Soybean germplasm from central and southern China for resistance has been evaluated by American researchers on a large scale. ...Soybean Phytophthora root rot (Phytophthora sojae) is a severe disease all over the world. Soybean germplasm from central and southern China for resistance has been evaluated by American researchers on a large scale. P. sojae has been found frequently in northeast of China in recent years, but not systematic evaluation of soybean germplasm for resistance has occurred there. By means of hypocotyl inoculation, 922 cultivars/lines from northeast of China were screened and evaluated for their response to race 1, and 25 of P. sojae. Generally resistance was less frequent in northeast of China than in central and southern China. Five cultivars/lines were identified that confer resistant responses to race 1, 3, 8, 25 and four additional isolates of P. sojae. These cultivars/lines may provide valuable sources of resistance for future breeding programs.展开更多
基金Supported by NaturalScience Foundation of Heilongjiang Province(No.C9912)
文摘A near-isogenic lines(NILs) -Williams and Williams82 is used to identify molecular marker linked to the resistance gene Rps1-k by RAPD. Genomic DNAs extracted from soybean leaves of the NILs were analyzed by RAPD using 160 different 10-nt random primers. Some specific DNA fragments were amplified from Williams82 with 4 primer(OPF-16, OPB-05, OPD-06 and OPH-05) which contains Rps1-k. All these specific DNA fragments were not detected in Williams. The experiment with OPH-05 was repeated 3 times and the results were the same. Using primer- OPH-05 to detect other resistance cultivars with Rps1-k, almost everyone can amplify the specific DNA fragment. So it is inferred that the specific DNA fragment is probably linked to Rps1-k.
基金Natural Science Foundation of Heilongjiang Province(C9912 )
文摘isolates of P.sojae were obtained from diseased soybean plants in 9 counties of Heilongjiang Province and 1 county of Jilin Province in Northeast of China.11 isolates were classified into 3 races;the rest that could not be classified due to intermediate reactions were classified into 12 virulence types,suggesting virulence diversity of P.sojae in China.Isolates represented race 1,3 and 8.Race 1 was the most prevalent one,and races 3 and 8 were the first report in China.Our results suggest P.sojae is aboriginal in China.
基金Studying Abroad and Coming Back Home Fund (LC02C08).
文摘To obtain the protein of duck interferon alpha and study its biological activities, the prokaryotic expression vector of DuIFN-αwas constructed and expressed in BL21 (DE3) plysS. Using PCR technique, the protein gene of DuIFN-αwas cloned from pMD-18-duIFN-αrecombinant. The gene was then inserted to pGEM-T vector and identified by restriction endonuclease analysis and sequencing. DuIFN-αwas ligated with the prokaryotic expression vector of pET30 a, then transformed into BL21 (DE3) plysS. The best inducing time and IPTG concentration for the expression of this recombinant protein was tested through the expression of the positive recombinant with different time span and different IPTG concentration. Lots of the protein of DuIFN-αwere expressed in BL21(DE3)plysS with 1 mmol·L-1 IPTG for 4 hours and its molecular weight for 34 000.
文摘Soybean Phytophthora root rot (Phytophthora sojae) is a severe disease all over the world. Soybean germplasm from central and southern China for resistance has been evaluated by American researchers on a large scale. P. sojae has been found frequently in northeast of China in recent years, but not systematic evaluation of soybean germplasm for resistance has occurred there. By means of hypocotyl inoculation, 922 cultivars/lines from northeast of China were screened and evaluated for their response to race 1, and 25 of P. sojae. Generally resistance was less frequent in northeast of China than in central and southern China. Five cultivars/lines were identified that confer resistant responses to race 1, 3, 8, 25 and four additional isolates of P. sojae. These cultivars/lines may provide valuable sources of resistance for future breeding programs.
