Objective:Our goal was to examine the impact of Zhuangshen Gujing decoction on proteinuria associated with chronic nephritis.Methods:72 patients with chronic nephritis proteinuria were divided randomly into two groups...Objective:Our goal was to examine the impact of Zhuangshen Gujing decoction on proteinuria associated with chronic nephritis.Methods:72 patients with chronic nephritis proteinuria were divided randomly into two groups;one group was treated with Zhuangshen Gujing(n=36;treatment group)and the other group was treated with irbesartan(n=36;control group).After 6 months’treatment,urine protein and renal function were evaluated.Results:Proteinuria resolved completely in four patients in the treatment group;complete disease resolution was observed only among three individuals in the control group.Of the 32 cases remaining in the treatment group,15 had substantial responses to Zhuangshen Gujing decoction,9 had partial responses,and 8 had no response to treatment;the overall the response rate was 73.33%.Among the 33 cases remaining in the control group,12 had a substantial response to irbesartan,5 had a partial response,and 16 had no response to treatment;the overall response rate among the controls was 57.67%(P=0.031).We also observed statistically significant differences with respect to quantitative comparisons of urinary protein at 24 days after a single course of treatment(P=0.001).Conclusion:Zhuangshen Gujing decoction is effective in treating chronic nephritis proteinuria.展开更多
With the rapid development of wind power, wind turbines are accompanied by a large quantity of power electronic converters connected to the grid, causing changes in the characteristics of the power system and leading ...With the rapid development of wind power, wind turbines are accompanied by a large quantity of power electronic converters connected to the grid, causing changes in the characteristics of the power system and leading to increasingly serious sub-synchronous oscillation (SSO) problems, which urgently require the generalized classification and characterization of the emerging oscillation problems. This paper classifies and characterizes the emerging types of SSO caused by grid-connected wind turbines to address these issues. Finally, the impact of the typical system parameters changes on the oscillation pattern is analyzed in depth to provide effective support for the subsequent suppression and prevention of SSO.展开更多
L1 is one of the largest offshore gas fields currently under development.In order to optimize the related design,nodal analysis is applied(including proper consideration of the plant productivity,sensitivity to the tu...L1 is one of the largest offshore gas fields currently under development.In order to optimize the related design,nodal analysis is applied(including proper consideration of the plant productivity,sensitivity to the tubing size,erosion effects,liquid carrying performance,and tubing string).As a result of such approach,it is shown that 13Cr material should be chosen as the appropriate tubing material.Moreover,3-1/2 inches 9.3 lb/ft N80 tubing,4-1/2 inches 12.75 lb/ft N80 tubing,5-1/2 inches 17 lb/ft N80 tubing should be used for a gas production rate under 80×10^(4)m^(3)/d,between 80×10^(4)m^(3)/d and 120×10^(4)m^(3)/d and above 120×10^(4)m^(3)/d,respectively.展开更多
Stomach adenocarcinoma (STAD) is the fifth most prevalent cancer and the third leading cause of cancer-related death in the world and is more common in Asia than in most Western countries. There is an urgent need to i...Stomach adenocarcinoma (STAD) is the fifth most prevalent cancer and the third leading cause of cancer-related death in the world and is more common in Asia than in most Western countries. There is an urgent need to identify potential novel oncogenes and tumor suppressor genes, and biomarkers for STAD. 6652 differentially expressed genes were identified between STAD and normal samples based on the transcriptome data analysis of the TCGA and GEO databases. 13 key modules were identified in STAD by WGCNA analysis. 293 potential STAD associated genes were identified from intersection by Venn Diagram. The 293 intersected genes were enriched in cell cortex and infection by GO and KEGG analysis. 10 hub genes were identified from PPI and Cytoscape analyses of the intersected genes. KLF4/CGN low and SHH/LIF high expression were associated with short overall survival of Asian STAD patients. Bioinformatics analysis revealed potential novel tumor suppressors (KLF4/CGN), oncogenes (SHH/LIF) and biomarkers for diagnosis, therapy and prognosis of STAD, specifically for Asian patients.展开更多
Oncolytic virus (OV) is a kind of virus that can preferentially infect and kill tumor cells. The second oncolytic virus drug was oncolytic herpes simplex virus (oHSV) Talimogene Laherparepvec (T-VEC). HSV-1 infectious...Oncolytic virus (OV) is a kind of virus that can preferentially infect and kill tumor cells. The second oncolytic virus drug was oncolytic herpes simplex virus (oHSV) Talimogene Laherparepvec (T-VEC). HSV-1 infectious cell culture protein 34.5 (ICP34.5) and latency-associated transcript (LAT) genes are closely related to virus selective infection and latent infection. Their engineering is essential for constructing efficient and safe oHSV. We summarized the mechanisms of ICP34.5 and LAT in the course of HSV-1 infection and reviewed the engineered oHSVs. We are aimed to provide an insight in developing oHSV in the future.展开更多
Acupuncture has reportedly improved memory and cognitive impairment in both animal and clinical studies. It may be an effective treatment for Alzheimer's disease (AD). The purpose of this meta-analysis was to revi...Acupuncture has reportedly improved memory and cognitive impairment in both animal and clinical studies. It may be an effective treatment for Alzheimer's disease (AD). The purpose of this meta-analysis was to review the effectiveness of acupuncture for the treatment of AD. Eight databases were searched for articles published up to and including July 2017, and 13 studies fulfilling the inclusion criteria were identified. The main outcomes assessed were clinical efficacy rate, Mini-Mental State Examination score, Ability of Daily Living Scale score, Alzheimer's Disease Assessment Scale-Cognition score, Hasegawa's Dementia Scale (HDS) score, and adverse events. The methodological quality of the articles was assessed using Cochrane's risk of bias. All the studies compared the efficacy of acupuncture with that of medication, and were published in Chinese journals. Meta-analysis revealed that acupuncture yielded positive results as determined via all the indexes scored except the HDS (95% CI -0.26 to 0.90, Z=0.35, P=0.73). Only one of the studies reported adverse events associated with acupuncture and medication. The rate of adverse events in the medication group was 13%. In most of the studies assessed in the current metaanalysis, acupuncture alone was better than conventional western medicines for the treatment of AD.展开更多
One-dimensional blast response of continuous-density graded cellular rods was investigated theoretically and numerically. Analytical model based on the rigid-plastic hardening(R-PH) model was used to predict the blast...One-dimensional blast response of continuous-density graded cellular rods was investigated theoretically and numerically. Analytical model based on the rigid-plastic hardening(R-PH) model was used to predict the blast response of density-graded cellular rods. Finite element(FE) analysis was performed using a new model based on the 3 D Voronoi technique. The FE results have a good agreement with the analytical predictions. The blast response and energy absorption of cellular rods with the same mass but different density distributions were examined under different blast loading. As a blast resistance structure, cellular materials with high energy absorption and low impulse transmit is attractive. However, high energy absorption and low impulse transmit cannot be achieved at the same time by changing the density distribution. The energy absorption capacity increases with the initial blast pressure and characteristic time of the exponentially decaying blast loading. By contract, when the blast loading exceeds the resistance capacity of cellular material, the transmitted stress will be enhanced which is detrimental to the structure being protected.展开更多
AIM To systematically evaluate the prognostic-predictive capability of Bcl-2 in colorectal cancer(CRC).METHODS A systematic literature search was conducted using Pub Med,Web of Science and EMBASE databases. Any eligib...AIM To systematically evaluate the prognostic-predictive capability of Bcl-2 in colorectal cancer(CRC).METHODS A systematic literature search was conducted using Pub Med,Web of Science and EMBASE databases. Any eligible study must meet the following criteria:(1) bcl-2 expression was evaluated in human CRC tissues by immunohistochemistry;(2) assessment of the relationships between bcl-2 expression and overall survival(OS),disease free survival(DFS),recurrent free survival(RFS) or clinic-pathological characteristics of CRC was included;(3) sufficient information was provided to estimate the hazard ratio(HR) or odds ratio and their 95% confidence intervals(CIs); and(4) the study was published in English. The impact of Bcl-2 expression on survival of CRC patients were evaluated through this meta-analysis.RESULTS A total of 40 eligible articles involving 7658 patients were enrolled in our final analysis. We drew the conclusion that Bcl-2 high expression was significantly correlated with favorable OS(pooled HR = 0.69,95%CI: 0.55-0.87,P = 0.002) and better DFS/RFS(pooled HR = 0.65,95%CI: 0.50-0.85,P = 0.001). Additionally,the subgroup analysis suggested that Bcl-2 overexpression was significantly associated withprognosis(OS) especially in patients came from Europe and America but not Asian and patients who did not receive any adjuvant therapy before surgery. Finally,our present results indicated that expression of bcl-2 protein was associated with high differentiation grade and A/B Ducks' stage. CONCLUSION Bcl-2 high expression was significantly correlated with favorable OS and better DFS/RFS. Hence,we propose that Bcl-2 may be a valuable prognostic-predictive marker in CRC.展开更多
The increasing threat of explosions on the battle field and the terrorist action requires the development of more effective blast resistance materials and structures.Curved structure can support the external loads eff...The increasing threat of explosions on the battle field and the terrorist action requires the development of more effective blast resistance materials and structures.Curved structure can support the external loads effectively by virtue of their spatial curvature.In review of the excellent energy absorption property of auxetic structure,employing auxetic structure as core material in curved sandwich shows the potential to improve the protection performance.In this study,a novel cylindrical sandwich panel with double arrow auxetic(DAA) core was designed and the numerical model was built by ABAQUS.Due to the complexity of the structure,systematic parameter study and optimal design are conducted.Two cases of optimal design were considered,case1 focuses on reducing the deflection and mass of the structure,while case2 focuses on reducing the deflection and increasing the energy absorption per unit mass.Parameter study and optimal design were conducted based on Latin Hypercube Sampling(LHD)method,artificial neural networks(ANN) metamodel and the nondominated sorting genetic algorithm(NSGA-Ⅱ).The Pareto front was obtained and the cylindrical DAA structure performed much better than its equal solid panel in both blast resistance and energy absorption capacity.Optimization results can be used as a reference for different applications.展开更多
Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease(HFMD) resulting in hundreds of deaths of children every year;However,currently,there is no effective treatment for EV71.In this study,the EV71 poly-p...Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease(HFMD) resulting in hundreds of deaths of children every year;However,currently,there is no effective treatment for EV71.In this study,the EV71 poly-protein(EV71-P1 protein) gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GS115.The EV71 P1 protein with a molecular weight of 100 kD was produced and secreted into the medium.The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70%.The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits.We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.展开更多
Annular grooved projectiles(AGPs)have drawn ongoing concerns as an advanced penetrator for their excellent anti-rebound capability in impacting metal plates.They could become embedded solidly in the target surface dur...Annular grooved projectiles(AGPs)have drawn ongoing concerns as an advanced penetrator for their excellent anti-rebound capability in impacting metal plates.They could become embedded solidly in the target surface during low-velocity impact.In this investigation,the firm embedding behavior of AGP was observed by impact experiments.Corresponding numerical simulations provided a better understanding of this process.Experimental and numerical results indicated that the firm embedding behavior of AGP was mainly due to the filling-material in the groove rather than the friction between the projectile and target,unlike traditional shape such as conical projectile.According to observation,firm embedding process can generally be subdivided into four stages:initial-cratering stage,groove-filling stage,fillingmaterial failure stage and rebound vibration stage.Moreover,the damage mechanics of target material around crater was obtained through microscopic tests.A comparison of the cross-sectional figures between the experiment and simulation proved that the analysis and the proposed method were reasonable and feasible,which further demonstrated that the firm embedding behavior has application potential in new concept warheads.展开更多
BACKGROUND: The peripheral morphologic characteristics of hepatocellular carcinoma (HCC) reflect tumor growth patterns.Computed tomography (CT) perfusion is a new method to analyze hemodynamic changes in tissues. We a...BACKGROUND: The peripheral morphologic characteristics of hepatocellular carcinoma (HCC) reflect tumor growth patterns.Computed tomography (CT) perfusion is a new method to analyze hemodynamic changes in tissues. We assessed the relationship between CT perfusion and histopathologic findings in the periphery of HCC lesions.METHODS: Non-contrast CT, enhanced dual-phase CT, and CT perfusion were performed on 77 subjects (47 patients and 30 controls). Based on the imaging findings of enhanced dualphase CT, the tumor edges were classified into three types: type I (sharp); type II (blurry); and type III (mixed). The CT perfusion parameters included hepatic blood flow, hepatic arterial fraction, hepatic arterial perfusion, and hepatic portal perfusion. The tissue sections from resected specimens were subjected to routine hematoxylin and eosin staining and immunohistochemical staining for CD34. The correlations between microvessel density (MVD) and the CT perfusion parameters were analyzed using Pearson's product-moment correlation coefficient. Changes in the perfusion parameters in tumor edges of different tumor types were evaluated.RESULTS: Type I (sharp): the pathologic findings showed fibrous connective tissue capsules in the tumor edges, and an MVD ≤ 30/mm2. Type II (blurry): the histology showed that the edges were clear with no capsules and an MVD >30/mm2. Type III(mixed): the pathology was similar to that of types I and II, and an MVD >30/mm2. Hepatic blood flow, hepatic arterial fraction, hepatic arterial perfusion, and hepatic portal perfusion were significantly increased in the tumor edges of HCC patients compared to those of the controls (P<0.05). The correlation between CT perfusion parameters and MVD was higher in blurry tumor edges of type II than in those of types I or III.CONCLUSION: CT perfusion imaging of tumor edges may be helpful in revealing histopathological features, and indirectly reflect angiogenic changes of HCCs.展开更多
基金funded by Key R&D projects of Chongqing Municipal Bureau of Science and Technology(No.CSTC2021jscxgksb-N0021)2022 Postgraduate intelligent medicine Special research and development plan of Chongqing Medical University(No.YJSZHYX202206).
文摘目的随着老龄化社会进程加速,老年人躯体衰弱和肌少症的患病率逐年上升,并带来诸多不良影响。膳食营养是防治躯体衰弱和肌少症的重要方式,但在社区环境中实施膳食营养干预受到诸多复杂因素影响。本综述旨在对相关研究中的促进和障碍因素进行系统综述,为制订相关健康管理方案提供参考。方法在Pub Med. .Web of Science 、Medline(Ovid). Embase(Ovid)和Cochrane Library 数据库进行检索,发表时间限定为建库至2023年1月。使用主题词和自由词组合完成检索。使用英国牛津大学循证医学中心质性研究质量评价工具(the Critical Appraisal Skills Program Qualiative Sudies Checklist)对 纳入研究进行质量评价。利用社会生态建模框架对提取的信息进行编码和分析。本综述方案已在PROSPERO注册(CRD42022381339)。结果共纳入10项研究。其中,4项研究仅进行营养相关干预,而膳食营养和运动干预相结合的有6项。根据社会生态模型,从个体特征、外部环境和干预措施3个层面对促进和障碍因素进行了总结,包含10个副主题。结论对社区中的躯体衰弱和肌少症老年人实施膳食营养干预措施时,应整合个人内部动机和外部支持,为参与者制订“量身定制”的干预方案,并最大程度地优化可利用的人力和物理资源。
文摘Objective:Our goal was to examine the impact of Zhuangshen Gujing decoction on proteinuria associated with chronic nephritis.Methods:72 patients with chronic nephritis proteinuria were divided randomly into two groups;one group was treated with Zhuangshen Gujing(n=36;treatment group)and the other group was treated with irbesartan(n=36;control group).After 6 months’treatment,urine protein and renal function were evaluated.Results:Proteinuria resolved completely in four patients in the treatment group;complete disease resolution was observed only among three individuals in the control group.Of the 32 cases remaining in the treatment group,15 had substantial responses to Zhuangshen Gujing decoction,9 had partial responses,and 8 had no response to treatment;the overall the response rate was 73.33%.Among the 33 cases remaining in the control group,12 had a substantial response to irbesartan,5 had a partial response,and 16 had no response to treatment;the overall response rate among the controls was 57.67%(P=0.031).We also observed statistically significant differences with respect to quantitative comparisons of urinary protein at 24 days after a single course of treatment(P=0.001).Conclusion:Zhuangshen Gujing decoction is effective in treating chronic nephritis proteinuria.
基金financially supported by the State Key Laboratory from Structural Chemistry, Chinese Academy of Sciences (20190008)the Basic Science and Technology Research Project of Wenzhou (G20190007, ZG2017027)financial support from BUCT-WZU Joint Fund
基金National Key Research and Development Program of China under Grant No.2017YFB0902002.
文摘With the rapid development of wind power, wind turbines are accompanied by a large quantity of power electronic converters connected to the grid, causing changes in the characteristics of the power system and leading to increasingly serious sub-synchronous oscillation (SSO) problems, which urgently require the generalized classification and characterization of the emerging oscillation problems. This paper classifies and characterizes the emerging types of SSO caused by grid-connected wind turbines to address these issues. Finally, the impact of the typical system parameters changes on the oscillation pattern is analyzed in depth to provide effective support for the subsequent suppression and prevention of SSO.
基金supported by the National Natural Science Foundation of China(Grant No.52174015)the Scientific Research Project of CNOOC(China)Co.,Ltd.“Research on key technologies for drilling and completion of 20 million cubic meters in Western Nanhai Oilfield”(No.CNOOC-KJ135ZDXM38ZJ05ZJ).
文摘L1 is one of the largest offshore gas fields currently under development.In order to optimize the related design,nodal analysis is applied(including proper consideration of the plant productivity,sensitivity to the tubing size,erosion effects,liquid carrying performance,and tubing string).As a result of such approach,it is shown that 13Cr material should be chosen as the appropriate tubing material.Moreover,3-1/2 inches 9.3 lb/ft N80 tubing,4-1/2 inches 12.75 lb/ft N80 tubing,5-1/2 inches 17 lb/ft N80 tubing should be used for a gas production rate under 80×10^(4)m^(3)/d,between 80×10^(4)m^(3)/d and 120×10^(4)m^(3)/d and above 120×10^(4)m^(3)/d,respectively.
基金the Natural Science Foundation of Shanghai,China(No.15ZR1428400)Shanghai Engineering Research Center of High-Performance Medical Device Materials,China(No.20DZ2255500)the Project of Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development,Tohoku University,sponsored by Ministry,Education,Culture,Sports,Science and Technology,Japan,and the Grant-in Aid for Scientific Research(C)(No.20K05139)from JSPS(Japan Society for the Promotion of Science),Tokyo,Japan.
文摘Stomach adenocarcinoma (STAD) is the fifth most prevalent cancer and the third leading cause of cancer-related death in the world and is more common in Asia than in most Western countries. There is an urgent need to identify potential novel oncogenes and tumor suppressor genes, and biomarkers for STAD. 6652 differentially expressed genes were identified between STAD and normal samples based on the transcriptome data analysis of the TCGA and GEO databases. 13 key modules were identified in STAD by WGCNA analysis. 293 potential STAD associated genes were identified from intersection by Venn Diagram. The 293 intersected genes were enriched in cell cortex and infection by GO and KEGG analysis. 10 hub genes were identified from PPI and Cytoscape analyses of the intersected genes. KLF4/CGN low and SHH/LIF high expression were associated with short overall survival of Asian STAD patients. Bioinformatics analysis revealed potential novel tumor suppressors (KLF4/CGN), oncogenes (SHH/LIF) and biomarkers for diagnosis, therapy and prognosis of STAD, specifically for Asian patients.
文摘Oncolytic virus (OV) is a kind of virus that can preferentially infect and kill tumor cells. The second oncolytic virus drug was oncolytic herpes simplex virus (oHSV) Talimogene Laherparepvec (T-VEC). HSV-1 infectious cell culture protein 34.5 (ICP34.5) and latency-associated transcript (LAT) genes are closely related to virus selective infection and latent infection. Their engineering is essential for constructing efficient and safe oHSV. We summarized the mechanisms of ICP34.5 and LAT in the course of HSV-1 infection and reviewed the engineered oHSVs. We are aimed to provide an insight in developing oHSV in the future.
基金Project(21471162) supported by the National Natural Science Foundation of ChinaProject(2015H6016) supported by the Science and Technology Project of Fujian Province,China
基金The study was supported by the National Natural Science Foundation of China (No.81574065).
文摘Acupuncture has reportedly improved memory and cognitive impairment in both animal and clinical studies. It may be an effective treatment for Alzheimer's disease (AD). The purpose of this meta-analysis was to review the effectiveness of acupuncture for the treatment of AD. Eight databases were searched for articles published up to and including July 2017, and 13 studies fulfilling the inclusion criteria were identified. The main outcomes assessed were clinical efficacy rate, Mini-Mental State Examination score, Ability of Daily Living Scale score, Alzheimer's Disease Assessment Scale-Cognition score, Hasegawa's Dementia Scale (HDS) score, and adverse events. The methodological quality of the articles was assessed using Cochrane's risk of bias. All the studies compared the efficacy of acupuncture with that of medication, and were published in Chinese journals. Meta-analysis revealed that acupuncture yielded positive results as determined via all the indexes scored except the HDS (95% CI -0.26 to 0.90, Z=0.35, P=0.73). Only one of the studies reported adverse events associated with acupuncture and medication. The rate of adverse events in the medication group was 13%. In most of the studies assessed in the current metaanalysis, acupuncture alone was better than conventional western medicines for the treatment of AD.
文摘One-dimensional blast response of continuous-density graded cellular rods was investigated theoretically and numerically. Analytical model based on the rigid-plastic hardening(R-PH) model was used to predict the blast response of density-graded cellular rods. Finite element(FE) analysis was performed using a new model based on the 3 D Voronoi technique. The FE results have a good agreement with the analytical predictions. The blast response and energy absorption of cellular rods with the same mass but different density distributions were examined under different blast loading. As a blast resistance structure, cellular materials with high energy absorption and low impulse transmit is attractive. However, high energy absorption and low impulse transmit cannot be achieved at the same time by changing the density distribution. The energy absorption capacity increases with the initial blast pressure and characteristic time of the exponentially decaying blast loading. By contract, when the blast loading exceeds the resistance capacity of cellular material, the transmitted stress will be enhanced which is detrimental to the structure being protected.
文摘AIM To systematically evaluate the prognostic-predictive capability of Bcl-2 in colorectal cancer(CRC).METHODS A systematic literature search was conducted using Pub Med,Web of Science and EMBASE databases. Any eligible study must meet the following criteria:(1) bcl-2 expression was evaluated in human CRC tissues by immunohistochemistry;(2) assessment of the relationships between bcl-2 expression and overall survival(OS),disease free survival(DFS),recurrent free survival(RFS) or clinic-pathological characteristics of CRC was included;(3) sufficient information was provided to estimate the hazard ratio(HR) or odds ratio and their 95% confidence intervals(CIs); and(4) the study was published in English. The impact of Bcl-2 expression on survival of CRC patients were evaluated through this meta-analysis.RESULTS A total of 40 eligible articles involving 7658 patients were enrolled in our final analysis. We drew the conclusion that Bcl-2 high expression was significantly correlated with favorable OS(pooled HR = 0.69,95%CI: 0.55-0.87,P = 0.002) and better DFS/RFS(pooled HR = 0.65,95%CI: 0.50-0.85,P = 0.001). Additionally,the subgroup analysis suggested that Bcl-2 overexpression was significantly associated withprognosis(OS) especially in patients came from Europe and America but not Asian and patients who did not receive any adjuvant therapy before surgery. Finally,our present results indicated that expression of bcl-2 protein was associated with high differentiation grade and A/B Ducks' stage. CONCLUSION Bcl-2 high expression was significantly correlated with favorable OS and better DFS/RFS. Hence,we propose that Bcl-2 may be a valuable prognostic-predictive marker in CRC.
文摘The increasing threat of explosions on the battle field and the terrorist action requires the development of more effective blast resistance materials and structures.Curved structure can support the external loads effectively by virtue of their spatial curvature.In review of the excellent energy absorption property of auxetic structure,employing auxetic structure as core material in curved sandwich shows the potential to improve the protection performance.In this study,a novel cylindrical sandwich panel with double arrow auxetic(DAA) core was designed and the numerical model was built by ABAQUS.Due to the complexity of the structure,systematic parameter study and optimal design are conducted.Two cases of optimal design were considered,case1 focuses on reducing the deflection and mass of the structure,while case2 focuses on reducing the deflection and increasing the energy absorption per unit mass.Parameter study and optimal design were conducted based on Latin Hypercube Sampling(LHD)method,artificial neural networks(ANN) metamodel and the nondominated sorting genetic algorithm(NSGA-Ⅱ).The Pareto front was obtained and the cylindrical DAA structure performed much better than its equal solid panel in both blast resistance and energy absorption capacity.Optimization results can be used as a reference for different applications.
基金Key Project of Science and Technology Department of Wuhan(zz2011-12)Key Project of the Educational Bureau of Wuhan(sz2011-13-10)+1 种基金Project of Science and Technology Department of Hubei(2010CDB04801)The State Key Laboratory program of Viral Genetic Engineering(2010KF10)
文摘Enterovirus type 71(EV71) causes severe hand-foot-and-mouth disease(HFMD) resulting in hundreds of deaths of children every year;However,currently,there is no effective treatment for EV71.In this study,the EV71 poly-protein(EV71-P1 protein) gene was processed and cloned into the eukaryotic expression vector pPIC9k and then expressed in Pichia pastoris strain GS115.The EV71 P1 protein with a molecular weight of 100 kD was produced and secreted into the medium.The soluble EV71 P1 protein was purified by column chromatography with a recovery efficiency of 70%.The result of the immunological analysis showed that the EV71 P1 protein had excellent immunogenicity and could stimulate the production of EV71-VP1 IgG antibody in injected rabbits.We suggest that EV71-P1 protein is an ideal candidate for an EV71 vaccine to prevent EV71 infection.
基金financially supported by the National Natural Science Foundation of China [grant number 11472053]
文摘Annular grooved projectiles(AGPs)have drawn ongoing concerns as an advanced penetrator for their excellent anti-rebound capability in impacting metal plates.They could become embedded solidly in the target surface during low-velocity impact.In this investigation,the firm embedding behavior of AGP was observed by impact experiments.Corresponding numerical simulations provided a better understanding of this process.Experimental and numerical results indicated that the firm embedding behavior of AGP was mainly due to the filling-material in the groove rather than the friction between the projectile and target,unlike traditional shape such as conical projectile.According to observation,firm embedding process can generally be subdivided into four stages:initial-cratering stage,groove-filling stage,fillingmaterial failure stage and rebound vibration stage.Moreover,the damage mechanics of target material around crater was obtained through microscopic tests.A comparison of the cross-sectional figures between the experiment and simulation proved that the analysis and the proposed method were reasonable and feasible,which further demonstrated that the firm embedding behavior has application potential in new concept warheads.
基金supported by grants from the National Nature Science Foundation of China (81471736)Heilongjiang Province Foundation for Returness (LC2013C38)
文摘BACKGROUND: The peripheral morphologic characteristics of hepatocellular carcinoma (HCC) reflect tumor growth patterns.Computed tomography (CT) perfusion is a new method to analyze hemodynamic changes in tissues. We assessed the relationship between CT perfusion and histopathologic findings in the periphery of HCC lesions.METHODS: Non-contrast CT, enhanced dual-phase CT, and CT perfusion were performed on 77 subjects (47 patients and 30 controls). Based on the imaging findings of enhanced dualphase CT, the tumor edges were classified into three types: type I (sharp); type II (blurry); and type III (mixed). The CT perfusion parameters included hepatic blood flow, hepatic arterial fraction, hepatic arterial perfusion, and hepatic portal perfusion. The tissue sections from resected specimens were subjected to routine hematoxylin and eosin staining and immunohistochemical staining for CD34. The correlations between microvessel density (MVD) and the CT perfusion parameters were analyzed using Pearson's product-moment correlation coefficient. Changes in the perfusion parameters in tumor edges of different tumor types were evaluated.RESULTS: Type I (sharp): the pathologic findings showed fibrous connective tissue capsules in the tumor edges, and an MVD ≤ 30/mm2. Type II (blurry): the histology showed that the edges were clear with no capsules and an MVD >30/mm2. Type III(mixed): the pathology was similar to that of types I and II, and an MVD >30/mm2. Hepatic blood flow, hepatic arterial fraction, hepatic arterial perfusion, and hepatic portal perfusion were significantly increased in the tumor edges of HCC patients compared to those of the controls (P<0.05). The correlation between CT perfusion parameters and MVD was higher in blurry tumor edges of type II than in those of types I or III.CONCLUSION: CT perfusion imaging of tumor edges may be helpful in revealing histopathological features, and indirectly reflect angiogenic changes of HCCs.