Objective:Tumor-associated macrophages(TAMs)of the M2 phenotype are frequently associated with cancer progression.Invasive cancer cells undergoing epithelial-mesenchymal transition(EMT)have a selective advantage as TA...Objective:Tumor-associated macrophages(TAMs)of the M2 phenotype are frequently associated with cancer progression.Invasive cancer cells undergoing epithelial-mesenchymal transition(EMT)have a selective advantage as TAM activators.Cyclin D1b is a highly oncogenic splice variant of cyclin D1.We previously reported that cyclin D1b enhances the invasiveness of breast cancer cells by inducing EMT.However,the role of cyclin D1b in inducing macrophage differentiation toward tumor-associated macrophage-like cells remains unknown.This study aimed to explore the relationship between breast cancer cells overexpressing cyclin Dlb and TAMs.Methods:Mouse breast cancer 4T1 cells were transfected with cyclin D1b variant and co-cultured with macrophage cells in a Transwell coculture system.The expression of characteristic cytokines in differentiated macrophages was detected using qRT-PCR,ELISA and zymography assay.Tumor-associated macrophage distribution in a transplanted tumor was detected by immunofluorescence staining.The proliferation and migration ability of breast cancer cells was detected using the cell counting kit-8(CCK-8)assay,wound healing assay,Transwell invasion assay,and lung metastasis assay.Expression levels of mRNAs were detected by qRT-PCR.Protein expression levels were detected by Western blotting.The integrated analyses of The Cancer Genome Atlas(TCGA)datasets and bioinformatics methods were adopted to discover gene expression,gene coexpression,and overall survival in patients with breast cancer.Results:After co-culture with breast cancer cells overexpressing cyclin D1b,RAW264.7 macrophages were differentiated into an M2 phenotype.Moreover,differentiated M2-like macrophages promoted the proliferation and migration of breast cancer cells in turn.Notably,these macrophages facilitated the migration of breast cancer cells in vivo.Further investigations indicated that differentiated M2-like macrophages induced EMT of breast cancer cells accompanied with upregulation of TGF-β1 and integrinβ3 expression.Conclusion:Breast cancer cells transfected with cyclin D1b can induce the differentiation of macrophages into a tumor-associated macrophage-like phenotype,which promotes tumor metastasis in vitro and in vivo.展开更多
The Prandtl-Tomlinson(PT)model is the most widely used and successful minimalistic model to describe atomistic scale friction.It can describe the thermally activated,stress assistant process which shows stick-slip fri...The Prandtl-Tomlinson(PT)model is the most widely used and successful minimalistic model to describe atomistic scale friction.It can describe the thermally activated,stress assistant process which shows stick-slip frictional behavior.The relationship between the energy barrier and lateral force is critical to determine how the frictional force depends on velocity and temperature.There is some confusion in the literature to derive such relationship.The underlying assumption and approximations are not stated in a clear way and the rigorous derivations are missing.This study discusses the asymptotic behavior of the energy barrier as the support-spring coupling lowers it to zero and gives a detailed derivation of the asymptotic expression of the energy barrier within the framework of PT model.展开更多
Monitoring the operating status of a High-Speed Train(HST) at any moment is necessary to ensure its security. Multi-channel vibration signals are collected by sensors installed on bogies and beneficial information are...Monitoring the operating status of a High-Speed Train(HST) at any moment is necessary to ensure its security. Multi-channel vibration signals are collected by sensors installed on bogies and beneficial information are extracted to determine the running condition. Based on multi-view clustering and considering different views of complementary information, this study proposes a Multi-view Kernel Fuzzy C-Means(MvKFCM) model for condition recognition of the HST bogie. First, fast Fourier transform coefficients of HST vibration signals of all channels are extracted. Then, the fuzzy classification coefficient of every channel is calculated after clustering to select the appropriate channels. Finally, the selected channels are used to cluster by MvKFCM and the conditions of HST are determined. Experimental results show that the selection is effective to maintain rich feature information and remove redundancy. Furthermore, the condition recognition rate of MvKFCM is higher than that of single-view and four other multiple-view clustering algorithms.展开更多
基金supported by the National Natural Science Foundation of China(No.81702920,No.82174020).
文摘Objective:Tumor-associated macrophages(TAMs)of the M2 phenotype are frequently associated with cancer progression.Invasive cancer cells undergoing epithelial-mesenchymal transition(EMT)have a selective advantage as TAM activators.Cyclin D1b is a highly oncogenic splice variant of cyclin D1.We previously reported that cyclin D1b enhances the invasiveness of breast cancer cells by inducing EMT.However,the role of cyclin D1b in inducing macrophage differentiation toward tumor-associated macrophage-like cells remains unknown.This study aimed to explore the relationship between breast cancer cells overexpressing cyclin Dlb and TAMs.Methods:Mouse breast cancer 4T1 cells were transfected with cyclin D1b variant and co-cultured with macrophage cells in a Transwell coculture system.The expression of characteristic cytokines in differentiated macrophages was detected using qRT-PCR,ELISA and zymography assay.Tumor-associated macrophage distribution in a transplanted tumor was detected by immunofluorescence staining.The proliferation and migration ability of breast cancer cells was detected using the cell counting kit-8(CCK-8)assay,wound healing assay,Transwell invasion assay,and lung metastasis assay.Expression levels of mRNAs were detected by qRT-PCR.Protein expression levels were detected by Western blotting.The integrated analyses of The Cancer Genome Atlas(TCGA)datasets and bioinformatics methods were adopted to discover gene expression,gene coexpression,and overall survival in patients with breast cancer.Results:After co-culture with breast cancer cells overexpressing cyclin D1b,RAW264.7 macrophages were differentiated into an M2 phenotype.Moreover,differentiated M2-like macrophages promoted the proliferation and migration of breast cancer cells in turn.Notably,these macrophages facilitated the migration of breast cancer cells in vivo.Further investigations indicated that differentiated M2-like macrophages induced EMT of breast cancer cells accompanied with upregulation of TGF-β1 and integrinβ3 expression.Conclusion:Breast cancer cells transfected with cyclin D1b can induce the differentiation of macrophages into a tumor-associated macrophage-like phenotype,which promotes tumor metastasis in vitro and in vivo.
基金supported by the National Science Foundation[1149704].
文摘The Prandtl-Tomlinson(PT)model is the most widely used and successful minimalistic model to describe atomistic scale friction.It can describe the thermally activated,stress assistant process which shows stick-slip frictional behavior.The relationship between the energy barrier and lateral force is critical to determine how the frictional force depends on velocity and temperature.There is some confusion in the literature to derive such relationship.The underlying assumption and approximations are not stated in a clear way and the rigorous derivations are missing.This study discusses the asymptotic behavior of the energy barrier as the support-spring coupling lowers it to zero and gives a detailed derivation of the asymptotic expression of the energy barrier within the framework of PT model.
基金supported in part by the National Natural Science Foundation of China (Nos. 61572407 and 61134002)
文摘Monitoring the operating status of a High-Speed Train(HST) at any moment is necessary to ensure its security. Multi-channel vibration signals are collected by sensors installed on bogies and beneficial information are extracted to determine the running condition. Based on multi-view clustering and considering different views of complementary information, this study proposes a Multi-view Kernel Fuzzy C-Means(MvKFCM) model for condition recognition of the HST bogie. First, fast Fourier transform coefficients of HST vibration signals of all channels are extracted. Then, the fuzzy classification coefficient of every channel is calculated after clustering to select the appropriate channels. Finally, the selected channels are used to cluster by MvKFCM and the conditions of HST are determined. Experimental results show that the selection is effective to maintain rich feature information and remove redundancy. Furthermore, the condition recognition rate of MvKFCM is higher than that of single-view and four other multiple-view clustering algorithms.
