Elucidating the intrinsic relationship between viscosity/H_(2)0_(2) and mitochondria-associated diseases remains a great challenge owing to the lack of research on multiple diseases models,such as inflammation and mal...Elucidating the intrinsic relationship between viscosity/H_(2)0_(2) and mitochondria-associated diseases remains a great challenge owing to the lack of research on multiple diseases models,such as inflammation and malignant tumor models.In this work,we have developed a mitochondria-specific orange/near-infrared-emissive fluorescent probe TTPB,for dual-imaging of viscosity and H_(2)0_(2) levels in two different channels.The probe exhibited a remarkable response to viscosity with NIR emission round 666 nm,and was highly sensitive to H_(2)0_(2) in orange channel with emission peak at 586 nm.Moreover,TTPB has good mitochondria-specific ability and permits individual detecting of viscosity in NIR channels and H_(2)0_(2) levels in orange channel in living cells.More notably,TTPB was successfully applied to simultaneously image the viscosity and H_(2)0_(2) levels in inflammation and cancer models.展开更多
基金the National Natural Science Foundation of China(Nos.81901814 and 21874087)Natural Science Foundation of Shanxi Province(No.201801D121040)The animal experiments were approved by the Animal Care and Use Committee of Shanxi University,and performed in compliance with the Animal Management Rules of the Ministry of Health of the People's Republic of China(Document no.55,2001).
文摘Elucidating the intrinsic relationship between viscosity/H_(2)0_(2) and mitochondria-associated diseases remains a great challenge owing to the lack of research on multiple diseases models,such as inflammation and malignant tumor models.In this work,we have developed a mitochondria-specific orange/near-infrared-emissive fluorescent probe TTPB,for dual-imaging of viscosity and H_(2)0_(2) levels in two different channels.The probe exhibited a remarkable response to viscosity with NIR emission round 666 nm,and was highly sensitive to H_(2)0_(2) in orange channel with emission peak at 586 nm.Moreover,TTPB has good mitochondria-specific ability and permits individual detecting of viscosity in NIR channels and H_(2)0_(2) levels in orange channel in living cells.More notably,TTPB was successfully applied to simultaneously image the viscosity and H_(2)0_(2) levels in inflammation and cancer models.
