The global outbreak of coronavirus disease 2019(COVID-19),which is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),as of 8 May 2021,has surpassed 150700000 infections and 3279000 deaths worldwide...The global outbreak of coronavirus disease 2019(COVID-19),which is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),as of 8 May 2021,has surpassed 150700000 infections and 3279000 deaths worldwide.Evidence indicates that SARS-CoV-2 RNA can be detected on particulate matter(PM),and COVID-19 cases are correlated with levels of air pollutants.However,the mechanisms of PM involvement in the spread of SARS-CoV-2 remain poorly understood.Here,we found that PM exposure increased the expression level of angiotensin-converting enzyme 2(ACE2)and transmembrane serine protease 2(TMPRSS2)in several epithelial cells and increased the adsorption of the SARS-CoV-2 spike protein.Instillation of PM in a hACE2 mouse model significantly increased the expression of ACE2 and Tmprss2 and viral replication in the lungs.Furthermore,PM exacerbated the pulmonary lesions caused by SARS-CoV-2 infection in the hACE2 mice.In conclusion,our study demonstrated that PM is an epidemiological factor of COVID-19,emphasizing the necessity of wearing anti-PM masks to cope with this global pandemic.展开更多
基金This work was supported by the National Natural Science Foundation of China(NSFC)(31900331,32070444)Science and Technology Department of Yunnan Province(202001AW070019)+2 种基金Chinese Academy of Sciences“Light of West China”Program and Youth Innovation Promotion Association(2019378)to L.J.and the NSFC(21761142002)Chinese Academy of Sciences(XDB31000000,SAJC201606 and KGFZD-135-17-011)Yunnan Province(2019-YT-053 and 2019ZF003)to R.L.and the NSFC(91643206)to W.J.D。
文摘The global outbreak of coronavirus disease 2019(COVID-19),which is caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),as of 8 May 2021,has surpassed 150700000 infections and 3279000 deaths worldwide.Evidence indicates that SARS-CoV-2 RNA can be detected on particulate matter(PM),and COVID-19 cases are correlated with levels of air pollutants.However,the mechanisms of PM involvement in the spread of SARS-CoV-2 remain poorly understood.Here,we found that PM exposure increased the expression level of angiotensin-converting enzyme 2(ACE2)and transmembrane serine protease 2(TMPRSS2)in several epithelial cells and increased the adsorption of the SARS-CoV-2 spike protein.Instillation of PM in a hACE2 mouse model significantly increased the expression of ACE2 and Tmprss2 and viral replication in the lungs.Furthermore,PM exacerbated the pulmonary lesions caused by SARS-CoV-2 infection in the hACE2 mice.In conclusion,our study demonstrated that PM is an epidemiological factor of COVID-19,emphasizing the necessity of wearing anti-PM masks to cope with this global pandemic.
