Objective:To investigate the role of natriuretic peptide in the process of left ventricular dysfunction caused by emotional stress.Methods:Adult male SD rats(n=30)and Wistar rats(n=60)were selected in this study.Ather...Objective:To investigate the role of natriuretic peptide in the process of left ventricular dysfunction caused by emotional stress.Methods:Adult male SD rats(n=30)and Wistar rats(n=60)were selected in this study.Atherosclerosis models were induced with high-fat diet and excess VD3 injection(eight consecutive weeks),and anger stress models were prepared by residentintruder stress experiment(two consecutive weeks).Furthermore,left ventricular functions were examined by high-resolution echocardiograph,after which left ventricular myocardium and coronary arteries were prepared for pathological section and observed with electron microscope.At the same time,the hypothalamus,medulla oblongata and left ventricular myocardium were also prepared for pathological sections to detect the localization and expression of AMP,BNP and NPK-A with immunofluorescence and western blot.Results:We found that left ventricular functions of atherosclerosis or emotional stress modeled rats were both inferior to the healthy ones and superior to the combined(atherosclerosis and emotional stress)modeled ones(P<0.05).We also found that atherosclerosis and emotional stress could both cause morphological changes of left ventricular cells and capillary which contribute to apoptosis and hyperblastosis.Further more,there was NPR—A distributed in hypothalamus,medulla oblongata,as well as left ventricular tissues with the same express trend between groups,with atherosclerosis modeled rats the highest and the healthy rats the lowest.Conclusions:The results of our study suggest that anger stress could cause an excess consumption of ANP,BNP and NPR-A in nervous and cardiovascular system which inhibit the compensatory self-repair function of atherosclerosis rats,leading to a promotion of fibrosis and lipid peroxidation,offering insight into the neuroendocrine mechanisms of left heart function obstacle.展开更多
Aging is closely related to physiology and disease development in animals.Gut microbiota varies with lifecycle and exerts profound influences on the host.To investigate gut microbial alterations during growth and matu...Aging is closely related to physiology and disease development in animals.Gut microbiota varies with lifecycle and exerts profound influences on the host.To investigate gut microbial alterations during growth and maturation,41 female cynomolgus monkeys(Macaca fascicularis)ranging in age from 1 month to 15 years were divided into four groups(infant,young,adult,and middle-aged).展开更多
Polyethyleneimine-polyethylene glycol （PEI-PEG）, a novel nanocarrier, has been used for trans- fection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene...Polyethyleneimine-polyethylene glycol （PEI-PEG）, a novel nanocarrier, has been used for trans- fection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene transfer in spiral ganglion cells. It remains unclear whether PEI-PEG could be used for gene therapy with X-linked inhibitor of apoptosis protein （XIAP） in the inner ear. In the present study, we performed PEI-PEG-mediated XIAP gene transfection in the cochlea of Sprague-Dawley rats, via scala tympani fenestration, before daily cisplatin injections. Audito- ry brainstem reflex tests demonstrated the protective effects of XIAP gene therapy on auditory function. Immunohistochemical staining revealed XIAP protein expression in the cytoplasm of cells in the spiral ganglion, the organ of Corti and the stria vascularis. Reverse transcription-PCR detected high levels of XIAP mRNA expression in the cochlea. The present findings suggest that PEI-PEG nanocarrier-mediated XIAP gene transfection results in XIAP expression in the cochlea, prevents damage to cochlear spiral ganglion cells, and protects hearing.展开更多
To address the discrepancy between carrier collection and light absorption of organic solar cells caused by the limited carrier mobility and optical absorption coefficient for the normally employed organic photoactive...To address the discrepancy between carrier collection and light absorption of organic solar cells caused by the limited carrier mobility and optical absorption coefficient for the normally employed organic photoactive layers,a light management structure composed of a front indium tin oxide(ITO)nanograting and ultrathin Al layer inserted in between the photoactive layer and the electron transport layer(ETL)is introduced.Owing to the antireflection and light scattering induced by the ITO nanograting and the suppression of light absorption in the ETL by the inserted Al layer,the light absorption of the photoactive layer is significantly enhanced in a spectral range from 400 nm to 650 nm that also covers the main energy region of solar irradiation for the normally employed active materials such as the P3HT:PC_(61)BM blend.The simulation results indicate that comparing with the control device with a planar configuration of ITO/PEDOT:PSS/P3HT:PC_(61)BM(80-nm thick)/ZnO/Al,the short-circuit current density and power conversion efficiency of the optimized light management structure can be improved by 32.86%and 34.46%.Moreover,good omnidirectional light management is observed for the proposed device structure.Owing to the fact that the light management structure possesses the simple structure and excellent performance,the exploration of such a structure can be believed to be significant in fabricating the thin film-based optoelectronic devices.展开更多
It is a challenge to selectively hydrogenate 4-nitrostyrene to 4-nitroethylbenzene,due to the similar energy barrier of hydrogenation of the nitro and vinyl groups.Herein,we demonstrate that such selective hydrogenati...It is a challenge to selectively hydrogenate 4-nitrostyrene to 4-nitroethylbenzene,due to the similar energy barrier of hydrogenation of the nitro and vinyl groups.Herein,we demonstrate that such selective hydrogenation can be achieved by Pd@Ru core–shell nanocubes that are pre-pared by epitaxial growth of a face-centered cubic Ru shell on Pd cubes.The core–shell structure of Pd@Ru nanocubes is conflrmed by transmission electron microscopy,X-ray diffraction spectroscopy,and elemental mapping mea-surements.It is found that the electronic structure and hence the catalytic activity of the Pd@Ru nanocubes can be readily modulated by the Ru shell thickness.This is manifested in electrochemical CO stripping measurements where a decrease of CO adsorption energy is observed on Pd@Ru nanocubes with the increase of the Ru shell thickness.Results from this study suggest that deliberate structural engineering can be exploited to prepare bimetallic core–shell nanostructures for highly active and selective hydrogenation of organic molecules with multi-functional moieties.展开更多
基金suppprted by Nature Science Fund of Hunan Province (No 20122345)
文摘Objective:To investigate the role of natriuretic peptide in the process of left ventricular dysfunction caused by emotional stress.Methods:Adult male SD rats(n=30)and Wistar rats(n=60)were selected in this study.Atherosclerosis models were induced with high-fat diet and excess VD3 injection(eight consecutive weeks),and anger stress models were prepared by residentintruder stress experiment(two consecutive weeks).Furthermore,left ventricular functions were examined by high-resolution echocardiograph,after which left ventricular myocardium and coronary arteries were prepared for pathological section and observed with electron microscope.At the same time,the hypothalamus,medulla oblongata and left ventricular myocardium were also prepared for pathological sections to detect the localization and expression of AMP,BNP and NPK-A with immunofluorescence and western blot.Results:We found that left ventricular functions of atherosclerosis or emotional stress modeled rats were both inferior to the healthy ones and superior to the combined(atherosclerosis and emotional stress)modeled ones(P<0.05).We also found that atherosclerosis and emotional stress could both cause morphological changes of left ventricular cells and capillary which contribute to apoptosis and hyperblastosis.Further more,there was NPR—A distributed in hypothalamus,medulla oblongata,as well as left ventricular tissues with the same express trend between groups,with atherosclerosis modeled rats the highest and the healthy rats the lowest.Conclusions:The results of our study suggest that anger stress could cause an excess consumption of ANP,BNP and NPR-A in nervous and cardiovascular system which inhibit the compensatory self-repair function of atherosclerosis rats,leading to a promotion of fibrosis and lipid peroxidation,offering insight into the neuroendocrine mechanisms of left heart function obstacle.
基金supported by the National Key Research and Development Program of China(2018YFC1003000)National Natural Science Foundation of China(31825018)+2 种基金Strategic Priority Research Program of the Chinese Academy of Sciences(XDB32060100)Shanghai Municipal Science and Technology Major Project(2018SHZDZX05)Shanghai Municipal Government Bureau of Science and Technology(18JC1410100)。
文摘Aging is closely related to physiology and disease development in animals.Gut microbiota varies with lifecycle and exerts profound influences on the host.To investigate gut microbial alterations during growth and maturation,41 female cynomolgus monkeys(Macaca fascicularis)ranging in age from 1 month to 15 years were divided into four groups(infant,young,adult,and middle-aged).
基金supported by the Natural Science Foundation of Guangdong Province in China,No.S2011040003553
文摘Polyethyleneimine-polyethylene glycol （PEI-PEG）, a novel nanocarrier, has been used for trans- fection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene transfer in spiral ganglion cells. It remains unclear whether PEI-PEG could be used for gene therapy with X-linked inhibitor of apoptosis protein （XIAP） in the inner ear. In the present study, we performed PEI-PEG-mediated XIAP gene transfection in the cochlea of Sprague-Dawley rats, via scala tympani fenestration, before daily cisplatin injections. Audito- ry brainstem reflex tests demonstrated the protective effects of XIAP gene therapy on auditory function. Immunohistochemical staining revealed XIAP protein expression in the cytoplasm of cells in the spiral ganglion, the organ of Corti and the stria vascularis. Reverse transcription-PCR detected high levels of XIAP mRNA expression in the cochlea. The present findings suggest that PEI-PEG nanocarrier-mediated XIAP gene transfection results in XIAP expression in the cochlea, prevents damage to cochlear spiral ganglion cells, and protects hearing.
基金supported by the Natural Science Foundation of Gansu Province,China(Grant No.20JR10RA611)the Fundamental Research Funds for Central Universities,China(Grant Nos.lzujbky-2017-178 and lzujbky-2017-181).
文摘To address the discrepancy between carrier collection and light absorption of organic solar cells caused by the limited carrier mobility and optical absorption coefficient for the normally employed organic photoactive layers,a light management structure composed of a front indium tin oxide(ITO)nanograting and ultrathin Al layer inserted in between the photoactive layer and the electron transport layer(ETL)is introduced.Owing to the antireflection and light scattering induced by the ITO nanograting and the suppression of light absorption in the ETL by the inserted Al layer,the light absorption of the photoactive layer is significantly enhanced in a spectral range from 400 nm to 650 nm that also covers the main energy region of solar irradiation for the normally employed active materials such as the P3HT:PC_(61)BM blend.The simulation results indicate that comparing with the control device with a planar configuration of ITO/PEDOT:PSS/P3HT:PC_(61)BM(80-nm thick)/ZnO/Al,the short-circuit current density and power conversion efficiency of the optimized light management structure can be improved by 32.86%and 34.46%.Moreover,good omnidirectional light management is observed for the proposed device structure.Owing to the fact that the light management structure possesses the simple structure and excellent performance,the exploration of such a structure can be believed to be significant in fabricating the thin film-based optoelectronic devices.
基金the Fundamental Research Funds for Central Universities(No.2019ZD22)Guangdong Innovative and Entrepreneurial Research Team Program(No.2016ZT06N569)the National Science Foundation for partial support of the work(No.CHE-2003685)。
文摘It is a challenge to selectively hydrogenate 4-nitrostyrene to 4-nitroethylbenzene,due to the similar energy barrier of hydrogenation of the nitro and vinyl groups.Herein,we demonstrate that such selective hydrogenation can be achieved by Pd@Ru core–shell nanocubes that are pre-pared by epitaxial growth of a face-centered cubic Ru shell on Pd cubes.The core–shell structure of Pd@Ru nanocubes is conflrmed by transmission electron microscopy,X-ray diffraction spectroscopy,and elemental mapping mea-surements.It is found that the electronic structure and hence the catalytic activity of the Pd@Ru nanocubes can be readily modulated by the Ru shell thickness.This is manifested in electrochemical CO stripping measurements where a decrease of CO adsorption energy is observed on Pd@Ru nanocubes with the increase of the Ru shell thickness.Results from this study suggest that deliberate structural engineering can be exploited to prepare bimetallic core–shell nanostructures for highly active and selective hydrogenation of organic molecules with multi-functional moieties.
