High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neur...High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neuronal damage.Proanthocyanidin,a potent antioxidant,has been shown to have neuroprotective effects.Proanthocyanidin B2 is a common dimer of oligomeric proanthocyanidins.To date,no studies have reported the neuroprotective effects of proanthocyanidin B2 against high-glucose-related neurotoxicity in dorsal root ganglion neurons.In this study,10 μg/m L proanthocyanidin B2 was used to investigate its effect on 45 m M high-glucose-cultured dorsal root ganglion neurons.We observed that challenge with high levels of glucose increased neuronal reactive oxygen species and promoted apoptosis,decreased cell viability,inhibited outgrowth of neurites,and decreased growth-associated protein 43 protein and m RNA levels.Proanthocyanidin B2 administration reversed the neurotoxic effects caused by glucose challenge.Blockage of the phosphatidylinositol 3 kinase/Akt signaling pathway with 10 μM LY294002 eliminated the protective effects of proanthocyanidin B2.Therefore,proanthocyanidin B2 might be a potential novel agent for the treatment of peripheral diabetic neuropathy.展开更多
Hydrophobic flocculation pretreatment was performed to assess its effect on the recovery of fine cuprite in sulfidation-flotation. The results of the micro-flotation experiment showed that cuprite recovery is related ...Hydrophobic flocculation pretreatment was performed to assess its effect on the recovery of fine cuprite in sulfidation-flotation. The results of the micro-flotation experiment showed that cuprite recovery is related to the particle size, and that an excessive content of fine particles(<18 μm) impacted the recovery of coarse particles. When hydrophobic flocculation pretreatment was used, the recovery of fine cuprite in sulfidation-flotation increased from 60.3% to 86.3% under optimum conditions(pH 9.5; sodium oleate concentration, 2 × 10^(-4) mol×L^(-1); stirring time, 6 min; stirring speed, 1600 r×min^(-1)). The laser particle size analysis and optical microscopy results indicate that hydrophobic flocculation pretreatment effectively reduces the content of fine cuprite, and augments the apparent particle size in the pulp. We performed the Derjaguin–Landau–Verwey–Overbeek(DLVO) theory and extended DLVO theory calculations to further support the interpretation of the results.展开更多
The effects of siderite on reverse flotation of hematite were investigated using micro flotation, adsorption tests, and Fourier transform infrared spectroscopy. The flotation results show that interactions between sid...The effects of siderite on reverse flotation of hematite were investigated using micro flotation, adsorption tests, and Fourier transform infrared spectroscopy. The flotation results show that interactions between siderite and quartz are the main reasons that siderite significantly influences the floatability. The interactions are attributed to dissolved siderite species and fine siderite particles. The interaction due to the dissolved species is, however, dominant. Derjaguin-Landau-Verwey-Overbeek（DLVO） theoretical calculations reveal that adhesion on quartz increases when the siderite particle size decreases and that fine particles partly influence quartz floatability. Chemical solution calculations indicate that the dissolved species of siderite might convert the surface of active quartz to CaCO_3 precipitates that can be depressed by starch. The theoretical calculations are in good agreement with the results of adsorption tests and FTIR spectroscopy and explain the reasons why siderite significantly influences reverse flotation of hematite.展开更多
基金supported by the National Natural Science Foundation of China,No.81501935the Shandong Provincial Natural Science Foundation of China,No.ZR2014HQ065
文摘High glucose affects primary afferent neurons in dorsal root ganglia by inhibiting neurite elongation,causing oxidative stress,and inducing neuronal apoptosis and mitochondrial dysfunction,which finally result in neuronal damage.Proanthocyanidin,a potent antioxidant,has been shown to have neuroprotective effects.Proanthocyanidin B2 is a common dimer of oligomeric proanthocyanidins.To date,no studies have reported the neuroprotective effects of proanthocyanidin B2 against high-glucose-related neurotoxicity in dorsal root ganglion neurons.In this study,10 μg/m L proanthocyanidin B2 was used to investigate its effect on 45 m M high-glucose-cultured dorsal root ganglion neurons.We observed that challenge with high levels of glucose increased neuronal reactive oxygen species and promoted apoptosis,decreased cell viability,inhibited outgrowth of neurites,and decreased growth-associated protein 43 protein and m RNA levels.Proanthocyanidin B2 administration reversed the neurotoxic effects caused by glucose challenge.Blockage of the phosphatidylinositol 3 kinase/Akt signaling pathway with 10 μM LY294002 eliminated the protective effects of proanthocyanidin B2.Therefore,proanthocyanidin B2 might be a potential novel agent for the treatment of peripheral diabetic neuropathy.
基金financially supported by the National Natural Science Foundation of China (Nos. 51374079 and 51504053) the Hundred, Thousand and Ten Thousand Talent Project of Liaoning Province (No. 2014921014)the Postdoctoral Science Foundation of China (No. 2015M571324)
文摘Hydrophobic flocculation pretreatment was performed to assess its effect on the recovery of fine cuprite in sulfidation-flotation. The results of the micro-flotation experiment showed that cuprite recovery is related to the particle size, and that an excessive content of fine particles(<18 μm) impacted the recovery of coarse particles. When hydrophobic flocculation pretreatment was used, the recovery of fine cuprite in sulfidation-flotation increased from 60.3% to 86.3% under optimum conditions(pH 9.5; sodium oleate concentration, 2 × 10^(-4) mol×L^(-1); stirring time, 6 min; stirring speed, 1600 r×min^(-1)). The laser particle size analysis and optical microscopy results indicate that hydrophobic flocculation pretreatment effectively reduces the content of fine cuprite, and augments the apparent particle size in the pulp. We performed the Derjaguin–Landau–Verwey–Overbeek(DLVO) theory and extended DLVO theory calculations to further support the interpretation of the results.
基金financially supported by the National Natural Science Foundation of China (No. 51374079)the Hundred, Thousand and Ten Thousand Talent Project of Liaoning Province (No. 2014921014)the Postdoctoral Science Foundation of China (No. 2015M571324)
文摘The effects of siderite on reverse flotation of hematite were investigated using micro flotation, adsorption tests, and Fourier transform infrared spectroscopy. The flotation results show that interactions between siderite and quartz are the main reasons that siderite significantly influences the floatability. The interactions are attributed to dissolved siderite species and fine siderite particles. The interaction due to the dissolved species is, however, dominant. Derjaguin-Landau-Verwey-Overbeek（DLVO） theoretical calculations reveal that adhesion on quartz increases when the siderite particle size decreases and that fine particles partly influence quartz floatability. Chemical solution calculations indicate that the dissolved species of siderite might convert the surface of active quartz to CaCO_3 precipitates that can be depressed by starch. The theoretical calculations are in good agreement with the results of adsorption tests and FTIR spectroscopy and explain the reasons why siderite significantly influences reverse flotation of hematite.
