Food safety problems caused by excessive nitrite addition have been frequently reported and the detection of nitrite in food is particularly important. The standing time during the pretreatment of primary sample has a...Food safety problems caused by excessive nitrite addition have been frequently reported and the detection of nitrite in food is particularly important. The standing time during the pretreatment of primary sample has a great influence on the concentration of nitrite tested by spectrophotometric method. In this context, three kinds of food samples are prepared, including canned mustard, canned fish and home-made pickled water. A series of standing times are placed during the sample pretreatments and the corresponding nitrite contents in these samples are detected by spectrophotometric method based on N-ethylenediamine dihydrochloride. This study aims to find out a reasonable standing time during the pretreatment of food sample, providing influence factor for precise detection of nitrite.展开更多
Burkholderia(B.)pseudomallei is a Gram-negative bacterium causing melioidosis,a tropical infection that is being recognized as a growing public health problem[1].Melioidosis,with mortality rates ranging from under 10%...Burkholderia(B.)pseudomallei is a Gram-negative bacterium causing melioidosis,a tropical infection that is being recognized as a growing public health problem[1].Melioidosis,with mortality rates ranging from under 10%to 40%,is a significant concern in Southeast Asia,Australia,and beyond[2].The underdiagnosis,particularly in rural areas,emphasizes the need for improved awareness,surveillance,and management,potentially classifying melioidosis as a neglected tropical disease[3].展开更多
China’s six tropical regions include Guangdong Province,Yunnan Province,Hainan Province,Hong Kong Special Administrative Region(SAR),Macao SAR,and Taiwan,China.Hainan,seated in the southernmost tropical region of Chi...China’s six tropical regions include Guangdong Province,Yunnan Province,Hainan Province,Hong Kong Special Administrative Region(SAR),Macao SAR,and Taiwan,China.Hainan,seated in the southernmost tropical region of China,is home to ticks that remain active throughout all four seasons.This heightens their potential to transmit tick-borne diseases to both animals and humans.This study provides a succinct overview of the prevailing tick species’spatial distribution and offers an outline of the range and dispersion of emerging tick-borne infections in tick vectors,animal hosts,and human populations within Hainan,China.展开更多
Dear Editor,Severe fever with thrombocytopenia syndrome(SFTS)is an emerging human disease with high mortality rates of up to 30%(Yu et al.2011;Li et al.2018,2021).The increasing incidence of SFTS led to great concern ...Dear Editor,Severe fever with thrombocytopenia syndrome(SFTS)is an emerging human disease with high mortality rates of up to 30%(Yu et al.2011;Li et al.2018,2021).The increasing incidence of SFTS led to great concern for public health in East Asia(Zhu et al.2019;Miao et al.2020).SFTS is caused by SFTS virus(SFTSV),a newly discovered tick-borne bunyavirus in the family Phenuiviridae.SFTSV was first identified in China in 2009(Yu et al.2011)and subsequently in South Korea(Kim et al.2013)and Japan(Takahashi et al.2014).展开更多
Background:Burkholderia pseudomallei is a tropical pathogen that causes melioidosis.Its intrinsic drug-resistance is a leading cause of treatment failure,and the few available antibiotics require prolonged use to be e...Background:Burkholderia pseudomallei is a tropical pathogen that causes melioidosis.Its intrinsic drug-resistance is a leading cause of treatment failure,and the few available antibiotics require prolonged use to be effective.This study aimed to assess the clinical potential of B.pseudomallei phages isolated from Hainan,China.Methods:Burkholderia pseudomallei strain(HNBPoo1)was used as the isolation host,and phages were recovered from domestic environmental sources,which were submitted to the host range determination,lytic property assays,and stability tests.The best candidate was examined via the transmission electron microscope for classification.With its genome sequenced and analyzed,its protective efficacy against B.pseudomallei infection in A549 cells and Caenorhabditis elegans was evaluated,in which cell viability and survival rates were compared using the one-way ANOVA method and the log-rank test.Results:A phage able to lyse 24/25 clinical isolates was recovered.It was classified in the Podoviridae family and was found to be amenable to propagation.Under the optimal multiplicity of infection(MOl)of o.1,an eclipse period of around 20 min and a high titer(io12 PFU/ml)produced within 1 h were demonstrated.This phage was found stabile at a wide range of temperatures(24,37,40,50,and 60 C)and pH values(3-12).After being designated as vB_BpP_HN01,it was fully sequenced,and the 71,398 bp linear genome,containing 93 open reading frames and a tRNA-Asn,displayed a low sequence similarity with known viruses.Additionally,protective effects of applications of vB_BpP_HNo1(MOl=0.1 and MOl=1)alone or in combination with antibiotics were found to improve viability of infected cells(70.6±6.8%,85.8±5.7%,91.9±1.8%,and 96.8±1.8%,respectively).A significantly reduced mortality(10%)and a decreased pathogen load were demonstrated in infected C.elegans following the addition of this phage.Conclusions:As the frst B.pseudomallei phage was isolated in Hainan,China,phage vB_BpP_HNO1 was characterized by promising lytic property,stability,and effciency of bacterial elimination during the in vitro/vivo experiments.Therefore,we can conclude that it is a potential alternative agent for combating melioidosis.展开更多
Studies on influenza virus by Chinese Academy of Sciences(CAS)could be traced back as early as 2005 by the CAS Key Laboratory of Pathogenic Microbiology and Immunology(CASPMI),who discovered that Qinghai-like Clade 2....Studies on influenza virus by Chinese Academy of Sciences(CAS)could be traced back as early as 2005 by the CAS Key Laboratory of Pathogenic Microbiology and Immunology(CASPMI),who discovered that Qinghai-like Clade 2.2H5N1 subtype highly pathogenic avian influenza virus(HPAIV)first caused severe outbreak in wild birds in Qinghai Lake(Liu et al.,2005).展开更多
文摘Food safety problems caused by excessive nitrite addition have been frequently reported and the detection of nitrite in food is particularly important. The standing time during the pretreatment of primary sample has a great influence on the concentration of nitrite tested by spectrophotometric method. In this context, three kinds of food samples are prepared, including canned mustard, canned fish and home-made pickled water. A series of standing times are placed during the sample pretreatments and the corresponding nitrite contents in these samples are detected by spectrophotometric method based on N-ethylenediamine dihydrochloride. This study aims to find out a reasonable standing time during the pretreatment of food sample, providing influence factor for precise detection of nitrite.
基金This work was supported by the Hainan Provincial Natural Science Foundation of China(824QN269)the Hainan Province Science and Technology Talent Innovation Project(KJRC2023D29)+1 种基金as well as the Major Science and Technology Program of Hainan Province(Grants No.ZDKJ202003 and ZDKJ2021036)the National Natural Science Foundation of China(Grants No.81960002 and 82370018).
文摘Burkholderia(B.)pseudomallei is a Gram-negative bacterium causing melioidosis,a tropical infection that is being recognized as a growing public health problem[1].Melioidosis,with mortality rates ranging from under 10%to 40%,is a significant concern in Southeast Asia,Australia,and beyond[2].The underdiagnosis,particularly in rural areas,emphasizes the need for improved awareness,surveillance,and management,potentially classifying melioidosis as a neglected tropical disease[3].
基金Supported by the Major Science and Technology Program of Hainan Province(grants ZDKJ202003,ZDKJ2021036,and SKJ10006)Additionally,financial support was received from the National Natural Science Foundation of China(grant 81960002)+1 种基金the Science and Technology Plan of Hainan Province(specifically,the Hainan Province Clinical Medical Center,SRC220002)The research benefited from a grant awarded by the Hainan Provincial Natural Science Foundation of High-Level Talent Project(821RC1152).
文摘China’s six tropical regions include Guangdong Province,Yunnan Province,Hainan Province,Hong Kong Special Administrative Region(SAR),Macao SAR,and Taiwan,China.Hainan,seated in the southernmost tropical region of China,is home to ticks that remain active throughout all four seasons.This heightens their potential to transmit tick-borne diseases to both animals and humans.This study provides a succinct overview of the prevailing tick species’spatial distribution and offers an outline of the range and dispersion of emerging tick-borne infections in tick vectors,animal hosts,and human populations within Hainan,China.
基金This study was supported by the Major Science and Technology Program of Hainan Province(ZDKJ202003)the National Natural Science Foundation of China(grants 82102433,32090011 and 32160124)+1 种基金the Hainan Provincial Natural Science Foundation of China(grants 822RC711 and 821QN254)State Key Research Development Program of China(grant 2019YFC1200504).We thank Ms.Qiuyu Zhao and Junyou Liu for helping in tick rearing.
文摘Dear Editor,Severe fever with thrombocytopenia syndrome(SFTS)is an emerging human disease with high mortality rates of up to 30%(Yu et al.2011;Li et al.2018,2021).The increasing incidence of SFTS led to great concern for public health in East Asia(Zhu et al.2019;Miao et al.2020).SFTS is caused by SFTS virus(SFTSV),a newly discovered tick-borne bunyavirus in the family Phenuiviridae.SFTSV was first identified in China in 2009(Yu et al.2011)and subsequently in South Korea(Kim et al.2013)and Japan(Takahashi et al.2014).
文摘Background:Burkholderia pseudomallei is a tropical pathogen that causes melioidosis.Its intrinsic drug-resistance is a leading cause of treatment failure,and the few available antibiotics require prolonged use to be effective.This study aimed to assess the clinical potential of B.pseudomallei phages isolated from Hainan,China.Methods:Burkholderia pseudomallei strain(HNBPoo1)was used as the isolation host,and phages were recovered from domestic environmental sources,which were submitted to the host range determination,lytic property assays,and stability tests.The best candidate was examined via the transmission electron microscope for classification.With its genome sequenced and analyzed,its protective efficacy against B.pseudomallei infection in A549 cells and Caenorhabditis elegans was evaluated,in which cell viability and survival rates were compared using the one-way ANOVA method and the log-rank test.Results:A phage able to lyse 24/25 clinical isolates was recovered.It was classified in the Podoviridae family and was found to be amenable to propagation.Under the optimal multiplicity of infection(MOl)of o.1,an eclipse period of around 20 min and a high titer(io12 PFU/ml)produced within 1 h were demonstrated.This phage was found stabile at a wide range of temperatures(24,37,40,50,and 60 C)and pH values(3-12).After being designated as vB_BpP_HN01,it was fully sequenced,and the 71,398 bp linear genome,containing 93 open reading frames and a tRNA-Asn,displayed a low sequence similarity with known viruses.Additionally,protective effects of applications of vB_BpP_HNo1(MOl=0.1 and MOl=1)alone or in combination with antibiotics were found to improve viability of infected cells(70.6±6.8%,85.8±5.7%,91.9±1.8%,and 96.8±1.8%,respectively).A significantly reduced mortality(10%)and a decreased pathogen load were demonstrated in infected C.elegans following the addition of this phage.Conclusions:As the frst B.pseudomallei phage was isolated in Hainan,China,phage vB_BpP_HNO1 was characterized by promising lytic property,stability,and effciency of bacterial elimination during the in vitro/vivo experiments.Therefore,we can conclude that it is a potential alternative agent for combating melioidosis.
基金supported by the National Key R&D Program of China(2016YFE0205800)National Science and Technology Major Project(2016ZX10004222)+5 种基金Emergency Technology Research Issue on Prevention and Control for Human Infection with A(H7N9)Avian Influenza Virus(10600100000015001206)intramural special grants for influenza virus research from the Chinese Academy of Sciences(KJZD-EWL15)Tianjin Research Program of the Application Foundation and Advanced Technology(14JCYBJC24400)the research project RFBR 17-04-01919a leading principal investigator of the NSFC Innovative Research Group(81621091)supported by the Youth Innovation Promotion Association of Chinese Academy of Sciences(CAS)(2017122)
文摘Studies on influenza virus by Chinese Academy of Sciences(CAS)could be traced back as early as 2005 by the CAS Key Laboratory of Pathogenic Microbiology and Immunology(CASPMI),who discovered that Qinghai-like Clade 2.2H5N1 subtype highly pathogenic avian influenza virus(HPAIV)first caused severe outbreak in wild birds in Qinghai Lake(Liu et al.,2005).
