目的探讨三七皂苷(panax notoginseng saponins,PNS)对激素性股骨头坏死(steonecrosis of the femoral head,SONFH)早期细胞凋亡的影响及其作用机制。方法选取健康成年新西兰兔24只,分为正常对照组、模型组及PNS组3组。模型组采用马血清...目的探讨三七皂苷(panax notoginseng saponins,PNS)对激素性股骨头坏死(steonecrosis of the femoral head,SONFH)早期细胞凋亡的影响及其作用机制。方法选取健康成年新西兰兔24只,分为正常对照组、模型组及PNS组3组。模型组采用马血清(10mL/kg)加激素(40mg/kg)的方法建立兔SONFH模型;PNS组在模型组基础上同时给予PNS(50mg/kg)干预;正常对照组常规饲养。2周后处死动物取双侧股骨头进行脱钙包埋,TUNEL染色观察细胞内凋亡变化,BCA法检测股骨头内caspase-3活性变化,免疫组化及Western blot检测各组股骨头内Bax及Bcl-2含量的变化。结果 TUNEL染色结果发现,模型组骨组织内可见大量凋亡细胞,PNS组骨组织内凋亡细胞明显减少(P<0.05);与模型组相比,经PNS预处理后股骨头组织内的caspase-3活性明显下降(P<0.05);免疫组化染色及Western blot检测结果证实,模型组骨组织内Bax蛋白表达量明显增加,而Bcl-2蛋白表达量明显减少,而与之相反的是PNS组骨组织内检测到的Bax蛋白表达量明显下降(P<0.05),而Bcl-2蛋白表达量明显增加(P<0.05)。结论PNS可能通过增强Bcl-2表达,降低Bax表达,抑制caspase-3活性,减少早期SONFH股骨头组织内的细胞凋亡。展开更多
Objective:To explore the suitable dosage of dexmedetomidine preemptive analgesia combined with transverse abdominal muscle block for postoperative analgesia of colon cancer.Methods:From March 2018 to October 2019,120 ...Objective:To explore the suitable dosage of dexmedetomidine preemptive analgesia combined with transverse abdominal muscle block for postoperative analgesia of colon cancer.Methods:From March 2018 to October 2019,120 patients undergoing laparoscopic radical resection of colon cancer in our hospital were randomly divided into control group(group C,without dexmedetomidine),low-dose group(group L,0.5μg/kg),medium-dose group(group M,1μg/kg)and high-dose group(group M,0.5μg/kg)The Mean arterial pressure,MAP),Heart rate,HR)and visual analog scale(vas)pain were compared at 2 h(T0),4 h(T1),8 h(T2),12 h(T3),24 h(T4)and 48 h(T5)after operation The pain sensitive area of mechanical stimulation was measured at T4,T5 and 72 h(T6)after operation.The adverse reactions of patients after operation were compared.Results:Compared with C group,MAP and HR of L,M and H groups decreased in different degrees at each time point.MAP and HR of m and h groups were lower than those of l group at different time points(P<0.05).The VAS score and pain sensitive area of T4-T6 in l,m and h groups were significantly lower than those in c group(P<0.05),but there was no significant difference in Ramsay score among groups(p>0.05).The vas score and pain sensitive area of m and h groups were lower than those of l group(P<0.05).Incidence of postoperative adverse reactions in l,m and h groups was lower than that in c group(P<0.05).Conclusion:Dexmedetomidine at doses of 1μg/kg and 1.5μg/kg has a good analgesic effect without increasing the incidence of adverse reactions.It is recommended that 1μg/kg Dexmedetomidine be used as an auxiliary drug dose of ropivacaine during TAP block.At the same time,it is necessary to strengthen monitoring to avoid adverse reactions such as hypotension and bradycardia.展开更多
Background The radial forearm skin flap(RFSF)was first introduced by the Chinese scholar Guofan Yang and has since been used for nearly 40 years.The many advantages of RFSF have been investigated in this study.Case pr...Background The radial forearm skin flap(RFSF)was first introduced by the Chinese scholar Guofan Yang and has since been used for nearly 40 years.The many advantages of RFSF have been investigated in this study.Case presentation In this case,a female patient underwent RFSF surgery in 1979.In 2016 and 2018,two interviews were held to evaluate her subjective postoperative experience.In addition,overall donor site evaluation was conducted based on general health checkups and tests;tests for appearance,tactile sensitivity,muscle strength,and motor function;and CTA.Results The flap survived well,and the donor site recovered without hand necrosis in the 1970s.In 2018,the patient was in good health condition and expressed her satisfaction with the surgery.The patient did not suffer from any postoperative complications such as diminished sensation of the donor site or donor site dysfunction;in the evaluation of hand and finger function,there was no point where functional reduction of the donor site was noted.CTA revealed compensatory blood supply with enlargement in the diameter of the ulnar and interosseous artery.Conclusion After a nearly 40-year follow-up,the patient who underwent the first free radial forearm flap transplantation expressed satisfaction with the operative outcomes.The examination showed good results at the recipient site with little donor site deformities and good compensatory blood supply.展开更多
文摘目的探讨三七皂苷(panax notoginseng saponins,PNS)对激素性股骨头坏死(steonecrosis of the femoral head,SONFH)早期细胞凋亡的影响及其作用机制。方法选取健康成年新西兰兔24只,分为正常对照组、模型组及PNS组3组。模型组采用马血清(10mL/kg)加激素(40mg/kg)的方法建立兔SONFH模型;PNS组在模型组基础上同时给予PNS(50mg/kg)干预;正常对照组常规饲养。2周后处死动物取双侧股骨头进行脱钙包埋,TUNEL染色观察细胞内凋亡变化,BCA法检测股骨头内caspase-3活性变化,免疫组化及Western blot检测各组股骨头内Bax及Bcl-2含量的变化。结果 TUNEL染色结果发现,模型组骨组织内可见大量凋亡细胞,PNS组骨组织内凋亡细胞明显减少(P<0.05);与模型组相比,经PNS预处理后股骨头组织内的caspase-3活性明显下降(P<0.05);免疫组化染色及Western blot检测结果证实,模型组骨组织内Bax蛋白表达量明显增加,而Bcl-2蛋白表达量明显减少,而与之相反的是PNS组骨组织内检测到的Bax蛋白表达量明显下降(P<0.05),而Bcl-2蛋白表达量明显增加(P<0.05)。结论PNS可能通过增强Bcl-2表达,降低Bax表达,抑制caspase-3活性,减少早期SONFH股骨头组织内的细胞凋亡。
基金National Natural Science Foundation of China(No.81803951)Guangdong Medical Science and Technology Foundation Project(No.B2013409)。
文摘Objective:To explore the suitable dosage of dexmedetomidine preemptive analgesia combined with transverse abdominal muscle block for postoperative analgesia of colon cancer.Methods:From March 2018 to October 2019,120 patients undergoing laparoscopic radical resection of colon cancer in our hospital were randomly divided into control group(group C,without dexmedetomidine),low-dose group(group L,0.5μg/kg),medium-dose group(group M,1μg/kg)and high-dose group(group M,0.5μg/kg)The Mean arterial pressure,MAP),Heart rate,HR)and visual analog scale(vas)pain were compared at 2 h(T0),4 h(T1),8 h(T2),12 h(T3),24 h(T4)and 48 h(T5)after operation The pain sensitive area of mechanical stimulation was measured at T4,T5 and 72 h(T6)after operation.The adverse reactions of patients after operation were compared.Results:Compared with C group,MAP and HR of L,M and H groups decreased in different degrees at each time point.MAP and HR of m and h groups were lower than those of l group at different time points(P<0.05).The VAS score and pain sensitive area of T4-T6 in l,m and h groups were significantly lower than those in c group(P<0.05),but there was no significant difference in Ramsay score among groups(p>0.05).The vas score and pain sensitive area of m and h groups were lower than those of l group(P<0.05).Incidence of postoperative adverse reactions in l,m and h groups was lower than that in c group(P<0.05).Conclusion:Dexmedetomidine at doses of 1μg/kg and 1.5μg/kg has a good analgesic effect without increasing the incidence of adverse reactions.It is recommended that 1μg/kg Dexmedetomidine be used as an auxiliary drug dose of ropivacaine during TAP block.At the same time,it is necessary to strengthen monitoring to avoid adverse reactions such as hypotension and bradycardia.
文摘Background The radial forearm skin flap(RFSF)was first introduced by the Chinese scholar Guofan Yang and has since been used for nearly 40 years.The many advantages of RFSF have been investigated in this study.Case presentation In this case,a female patient underwent RFSF surgery in 1979.In 2016 and 2018,two interviews were held to evaluate her subjective postoperative experience.In addition,overall donor site evaluation was conducted based on general health checkups and tests;tests for appearance,tactile sensitivity,muscle strength,and motor function;and CTA.Results The flap survived well,and the donor site recovered without hand necrosis in the 1970s.In 2018,the patient was in good health condition and expressed her satisfaction with the surgery.The patient did not suffer from any postoperative complications such as diminished sensation of the donor site or donor site dysfunction;in the evaluation of hand and finger function,there was no point where functional reduction of the donor site was noted.CTA revealed compensatory blood supply with enlargement in the diameter of the ulnar and interosseous artery.Conclusion After a nearly 40-year follow-up,the patient who underwent the first free radial forearm flap transplantation expressed satisfaction with the operative outcomes.The examination showed good results at the recipient site with little donor site deformities and good compensatory blood supply.
