Drug resistance renders standard chemotherapy ineffective in the treatment of connective tissue growth factor （CTGF）-overexpressing breast cancer. By co-embedding the breast tumor cell-penetrating peptide （PEGA-pVE...Drug resistance renders standard chemotherapy ineffective in the treatment of connective tissue growth factor （CTGF）-overexpressing breast cancer. By co-embedding the breast tumor cell-penetrating peptide （PEGA-pVEC） and hyaluronic acid （HA） as a targeting media, novel cascaded targeting nanoparficles （HACT NPs） were created on a rattle mesoporous silica （rmSiO2） scaffold for the pinpoint delivery of siRNAs along with an anticancer drug, aiming at overcoming the drug resistance of CTGF-overexpressing breast cancer in vivo. The targeting nanoparticles selectively accumulated in the vasculature under the guidance of the PEGA-pVEC peptide, cascaded by receptor-mediated endocytosis with the aid of another targeting agent, HA, presenting a greater in vivo tumor targeting ability than single targeting ligand vectors. In addition, an HA shell prevented the leakage of therapeutic drugs during the cargo transport process, until the hyaluronidase （HAase）-triggered degradation upon lysosomes entering, guaranteeing a controllable drug release inside the target cells. When the protective shell disintegrates, the released siRNA took charge to silence the gene associated with drug resistance, CTGF, thus facilitating doxorubicin-induced apoptosis. The cascaded targeting media （PEGA-pVEC and HA） advances precision-guided therapy in vivo, while the encapsulation of siRNAs into a chemotherapy drug delivery system provides an efficient strategy for the treatment of drug resistance cancers.展开更多
The increasingly severe energy crisis has strengthened the determination to develop environmentally friendly energy.And hydrogen has emerged as a candi-date for clean energy.Among many hydrogen generation methods,bioh...The increasingly severe energy crisis has strengthened the determination to develop environmentally friendly energy.And hydrogen has emerged as a candi-date for clean energy.Among many hydrogen generation methods,biohydrogen stands out due to its environmental sustainability,simple operating environ-ment,and cost advantages.This review focuses on the rational design of catalysts for fermentative hydrogen production.The principles of microbial dark fermen-tation and photo-fermentation are elucidated exhaustively.Various strategies to increase the efficiency of fermentative hydrogen production are summa-rized,and some recent representative works from microbial dark fermentation and photo-fermentation are described.Meanwhile,perspectives and discussions on the rational design of catalysts for fermentative hydrogen production are provided.展开更多
文摘Drug resistance renders standard chemotherapy ineffective in the treatment of connective tissue growth factor （CTGF）-overexpressing breast cancer. By co-embedding the breast tumor cell-penetrating peptide （PEGA-pVEC） and hyaluronic acid （HA） as a targeting media, novel cascaded targeting nanoparficles （HACT NPs） were created on a rattle mesoporous silica （rmSiO2） scaffold for the pinpoint delivery of siRNAs along with an anticancer drug, aiming at overcoming the drug resistance of CTGF-overexpressing breast cancer in vivo. The targeting nanoparticles selectively accumulated in the vasculature under the guidance of the PEGA-pVEC peptide, cascaded by receptor-mediated endocytosis with the aid of another targeting agent, HA, presenting a greater in vivo tumor targeting ability than single targeting ligand vectors. In addition, an HA shell prevented the leakage of therapeutic drugs during the cargo transport process, until the hyaluronidase （HAase）-triggered degradation upon lysosomes entering, guaranteeing a controllable drug release inside the target cells. When the protective shell disintegrates, the released siRNA took charge to silence the gene associated with drug resistance, CTGF, thus facilitating doxorubicin-induced apoptosis. The cascaded targeting media （PEGA-pVEC and HA） advances precision-guided therapy in vivo, while the encapsulation of siRNAs into a chemotherapy drug delivery system provides an efficient strategy for the treatment of drug resistance cancers.
基金W.Z.would like to acknowledge the support from National Natural Science Foundation of China(grant number:22176086)Natural Science Foundation of Jiangsu Province(grant number:BK20210189)+2 种基金State Key laboratory of Pollution Control and Resource Reuse(grant number:PCRR-ZZ-202106)the Fundamental Research Funds for the Central Universities(grant number:021114380183 and 021114380189)the Research Funds from Frontiers Science Center for Critical Earth Material Cycling of Nanjing University,and Research Funds for Jiangsu Distinguished Professor.Y.L.would like to acknowledge the start-up fund from Washington State University.
文摘The increasingly severe energy crisis has strengthened the determination to develop environmentally friendly energy.And hydrogen has emerged as a candi-date for clean energy.Among many hydrogen generation methods,biohydrogen stands out due to its environmental sustainability,simple operating environ-ment,and cost advantages.This review focuses on the rational design of catalysts for fermentative hydrogen production.The principles of microbial dark fermen-tation and photo-fermentation are elucidated exhaustively.Various strategies to increase the efficiency of fermentative hydrogen production are summa-rized,and some recent representative works from microbial dark fermentation and photo-fermentation are described.Meanwhile,perspectives and discussions on the rational design of catalysts for fermentative hydrogen production are provided.
