Meiotic recombination is carried out through a special- ized pathway for the formation and repair of DNA dou- ble-strand breaks （DSBs） made by the Spo11 protein. The present study shed light on the functional role o...Meiotic recombination is carried out through a special- ized pathway for the formation and repair of DNA dou- ble-strand breaks （DSBs） made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression level during meiosis process. Knocking out the CYC2 gene results in arrest of meiotic conjugation process at 2.5-3.5 h after conjugation initiation, before the meiosis division starts, and in company with the absence of DSBs. To investigate the underlying mechanism of this phenomenon, a complete transcriptome profile was performed between wild-type strain and CYC2 knock-out strain. Functional analysis of RNA-Seq results identifies related differentially expressed genes （DEGs） including SP011 and these DEGs are enriched in DNA repair/mismatch repair （MMR） terms in homologous recombination （HR）, which indicates that CYC2 could play a crucial role in meiosis by regulating SP011 and participating in HR.展开更多
文摘Meiotic recombination is carried out through a special- ized pathway for the formation and repair of DNA dou- ble-strand breaks （DSBs） made by the Spo11 protein. The present study shed light on the functional role of cyclin, CYC2, in Tetrahymena thermophila which has transcriptionally high expression level during meiosis process. Knocking out the CYC2 gene results in arrest of meiotic conjugation process at 2.5-3.5 h after conjugation initiation, before the meiosis division starts, and in company with the absence of DSBs. To investigate the underlying mechanism of this phenomenon, a complete transcriptome profile was performed between wild-type strain and CYC2 knock-out strain. Functional analysis of RNA-Seq results identifies related differentially expressed genes （DEGs） including SP011 and these DEGs are enriched in DNA repair/mismatch repair （MMR） terms in homologous recombination （HR）, which indicates that CYC2 could play a crucial role in meiosis by regulating SP011 and participating in HR.
