The CBL-interacting protein kinase OsCIPK1 phosphorylated by SAPK10 positively regulates responses to ABA and osmotic stress in rice

SubclassⅢsucrose nonfermenting1-related protein kinase 2s(SnRK2s)function in ABA and abiotic stress responses by unknown mechanisms.We found that osmotic stress/ABA-activated protein kinase 10(SAPK10),a member of rice SnRK2s,physically interacted with CBL-interacting protein kinase 1(OsCIPK1).OsCIPK1 expression was up-regulated by ABA and PEG treatment,and overexpression increased the ABA sensitivity of seed germination and root growth and plant osmotic stress tolerance.Osmotic stress or ABA-induced activation of OsCIPK1 is dependent on SAPK10.SAPK10 phosphorylates Thr-24 of OsCIPK1 in vitro,and this phosphorylation increases the activity of OsCIPK1 and positively regulates the function of OsCIPK1 in ABA responses and plant osmotic stress tolerance.This study suggests that OsCIPK1 is a direct phosphorylated substrate of SAPK10,and SAPK10-mediated phosphorylation of OsCIPK1 functions in ABA signaling and increases rice osmotic stress tolerance.

周围神经损伤对骨髓间充质干细胞增殖及成骨分化影响的研究

目的:通过建立坐骨神经损伤模型,观察周围神经损伤对骨髓间充质干细胞(BMSCs)生长增殖及成骨分化的影响。方法:取4周龄、健康、SPF级雄性SD大鼠20只,建立一侧下肢坐骨神经损伤模型,术后4周分别提取健侧(对照组)和术侧(实验组)骨髓间充质干细胞,进行体外培养增殖,采用流式细胞术检测BMSCs表面标志物,MTT实验检测2组细胞的活性;同时取P3代细胞行成骨诱导分化,并通过碱性磷酸酶(ALP)活性检测、茜素红染色对比2组细胞成骨分化能力。结果:流式细胞术检测提示2组细胞CD90阳性,CD11b阴性;MTT实验结果显示实验组BMSCs生长速度相对较慢,较迟进入平台期;成骨诱导分化后,实验组钙化结节、ALP活性的吸光度值均低于对照组,差异具有统计学意义(P<0.05)。结论:周围神经损伤可抑制BMSCs增殖及其向成骨细胞的分化。