[Objectives]To explore the mechanism of Gegen Qinlian Decoction in treating type 2 diabetes mellitus(T2DM)complicated with non-alcoholic fatty liver disease(NAFLD)by analyzing the effective components of Gegen Qinlian...[Objectives]To explore the mechanism of Gegen Qinlian Decoction in treating type 2 diabetes mellitus(T2DM)complicated with non-alcoholic fatty liver disease(NAFLD)by analyzing the effective components of Gegen Qinlian Decoction.[Methods]TCMSP database was used to analyze the active components of Gegen Qinlian Decoction,and pubchem and Swiss ADME databases were also used to predict drug targets,extract T2DM complicated with NAFLD targets from OMIM and Genecards databases.Venny plot was drawn to obtain intersection targets,and finally Cytoscape was used to make core target maps and drug-target-disease network maps.Using DAVID and Metascape database to analyze the intersection targets,the gene ontology information of Go and KEGG was obtained.Microbial informatics technology was used to visualize GO,and Cytoscape was used to make drug-target-disease network map-enrichment pathway map.[Results]The network pharmacological analysis showed that Gegen Qinlian Decoction acted on the key targets of type 2 diabetes mellitus complicated with non-alcoholic fatty liver disease,such as ALB and ALT1,through many components,and achieved the purpose of treating this disease.The chemical constituents of the drug include formononetin,5-hydroxyisomucronulatol-2,5-2-O-glucoside,cholesteryl laurate,isoliquiritigenin,etc.[Conclusions]This study provides a new idea and theoretical support for future drug research and clinical practice.展开更多
As an important subcellular organelle for plant photosynthesis, chloroplast proteome research has become an important branch and hot issue of plant pro- teonfics. However, no efficient method has been established for ...As an important subcellular organelle for plant photosynthesis, chloroplast proteome research has become an important branch and hot issue of plant pro- teonfics. However, no efficient method has been established for extracting chloroplast proteome from plant leaves. In this paper, recent advances in extraction meth- ods of plant chloroplast proteome were summarized, which laid solid foundation for establishing efficient and practicable extraction methods of chloroplast proteome.展开更多
Cancer stem cells(CSCs)are considered tumor-initiating cells and the main drivers of disease progression.Targeting these rare cancer cells,however,remains challenging with respect to therapeutic benefit.Here,we report...Cancer stem cells(CSCs)are considered tumor-initiating cells and the main drivers of disease progression.Targeting these rare cancer cells,however,remains challenging with respect to therapeutic benefit.Here,we report the up-regulation of IL-13RA2 expression in colorectal cancer(CRC)tissues and spheroid cells.The expression of IL-13RA2 was positively correlated with canonical stemness markers in CRC.We further demonstrated that the level of IL-13 was up-regulated in the serum of CRC patients.Biologically,recombinant IL-13(rIL13)stimulation promoted the sphere formation,proliferation,and migration of CRC cells in vitro and enhanced tumorigenesis in vivo.This phenotype could be reversed by knocking down IL-13RA2.Mechanistically,IL-13 activated autophagy by inducing LC3I/LC3II transformation in CRC-CSCs,which was crucial for the biological functions of IL-13.We further demonstrated that IL-13RA2 acted as a modular link of the E3 ligase UBE3C and the substrate p53 protein,enhancing the interaction of UBE3C and p53,thereby inducing the K48-linked ubiquitination of p53.In conclusion,the IL-13/IL-13RA2 signaling cascade promotes CRC-CSC self-renewal and tumorigenesis by inducing p53 ubiquitination,adding an important layer to the connection between IL-13 and p53,which can be translated into novel targeted therapies.展开更多
The carbon isotope ratios(δ^(13)C) of modern land plants have been widely studied,and they provide valuable information about modern and past atmospheric,ecological and climatic changes [1].According to their differe...The carbon isotope ratios(δ^(13)C) of modern land plants have been widely studied,and they provide valuable information about modern and past atmospheric,ecological and climatic changes [1].According to their different photosynthetic pathways,land plants are mostly C_(3) and C_(4) plants.The δ^(13)C values of C_(3) plants are mainly within the range of-20‰ to-34‰.展开更多
Introduction:Recent data indicate a year-on-year increase in the proportion of human immunodeficiency virus/acquired immunodeficiency syndrome(HIV/AIDS)cases reported among individuals aged≥50 in China.This study com...Introduction:Recent data indicate a year-on-year increase in the proportion of human immunodeficiency virus/acquired immunodeficiency syndrome(HIV/AIDS)cases reported among individuals aged≥50 in China.This study compares the epidemiological characteristics of HIV/AIDS cases in populations above and below 50 years of age.By doing so,it seeks to scrutinize the current epidemiological landscape of HIV within these distinct age cohorts and suggest tailored interventions for each group.Methods:We utilized data from the Chinese HIV/AIDS Comprehensive Response Information Management System for our comparative analysis.Joinpoint regression analysis was employed to assess the trends in standardized detection rates.Results:In China,the number of newly reported HIV/AIDS cases in the 15-49 age group increased from 51,436 in 2010 to 55,397 in 2022,while it increased from 11,751 in 2010 to 51,856 in 2022 in the group aged≥50 years.Recent years have seen a greater proportion and detection rate of HIV/AIDS cases among the≥50 age demographic compared to the 15-49 age group.In 2022,significant statistical differences were observed between males and females in both age cohorts with respect to education,marital status,occupation,mode of transmission,location of diagnosis,and region.Conclusions:The observed trend of a rising proportion of HIV/AIDS cases in individuals aged 50 years and older necessitates heightened attention.It is imperative that we develop and implement interventions specifically designed to prevent and control the transmission of HIV within this demographic.展开更多
Plant immunity must be tightly controlled to avoid activation of defense mechanisms in the absence of pathogen attack. Protein phosphorylation is a common mechanism regulating immune signaling. In Arabidopsis thaliana...Plant immunity must be tightly controlled to avoid activation of defense mechanisms in the absence of pathogen attack. Protein phosphorylation is a common mechanism regulating immune signaling. In Arabidopsis thaliana, nine members of the type one protein phosphatase(TOPP) family(also known as protein phosphatase 1, PP1) have been identified. Here, we characterized the autoimmune phenotype of topp4-1, a previously identified dominant-negative mutant of TOPP4. Epistasis analysis showed that defense activation in topp4-1 depended on NON-RACE-SPECIFIC DISEASE RESISTANCE1,PHYTOALEXIN DEFICIENT4, and the salicylic acid pathway.We generated topp1/4/5/6/7/8/9 septuple mutants to investigate the function of TOPPs in plant immunity. Elevated defense gene expression and enhanced resistance to Pseudomonas syringae pv. tomato(Pst) DC3000 in the septuple mutant indicate that TOPPs function in plant defense responses. Furthermore, TOPPs physically interacted with mitogen-activated protein kinases(MAPKs)and affected the MAPK-mediated downstream defense pathway. Thus, our study reveals that TOPPs are important regulators of plant immunity.展开更多
Summary What is already known about this topic?Significant changes in human immunodeficiency virus(HIV)transmission modes have occurred in China,and the proportion of heterosexual transmission increased in recent year...Summary What is already known about this topic?Significant changes in human immunodeficiency virus(HIV)transmission modes have occurred in China,and the proportion of heterosexual transmission increased in recent years.What is added by this report?The proportions of diverse transmission routes and subgroups of heterosexual transmission were analyzed by provincial-level administrative divisions(PLADs).展开更多
Summary What is already known about this topic?The annual rates of newly diagnosed cases of human immunodeficiency virus(HIV)are increasing in China,yet the annual number of newly diagnosed cases of HIV infected throu...Summary What is already known about this topic?The annual rates of newly diagnosed cases of human immunodeficiency virus(HIV)are increasing in China,yet the annual number of newly diagnosed cases of HIV infected through injection drug use(IDU)is decreasing.展开更多
基金Guangxi Key R&D Program Project(GuiKe AB18221095)National and Autonomous Region-Level College Student Innovation and Entrepreneurship Training Funding Project(202210599009)High-level Talent Research Project of Youjiang Medical University for Nationalities(01002018079).
文摘[Objectives]To explore the mechanism of Gegen Qinlian Decoction in treating type 2 diabetes mellitus(T2DM)complicated with non-alcoholic fatty liver disease(NAFLD)by analyzing the effective components of Gegen Qinlian Decoction.[Methods]TCMSP database was used to analyze the active components of Gegen Qinlian Decoction,and pubchem and Swiss ADME databases were also used to predict drug targets,extract T2DM complicated with NAFLD targets from OMIM and Genecards databases.Venny plot was drawn to obtain intersection targets,and finally Cytoscape was used to make core target maps and drug-target-disease network maps.Using DAVID and Metascape database to analyze the intersection targets,the gene ontology information of Go and KEGG was obtained.Microbial informatics technology was used to visualize GO,and Cytoscape was used to make drug-target-disease network map-enrichment pathway map.[Results]The network pharmacological analysis showed that Gegen Qinlian Decoction acted on the key targets of type 2 diabetes mellitus complicated with non-alcoholic fatty liver disease,such as ALB and ALT1,through many components,and achieved the purpose of treating this disease.The chemical constituents of the drug include formononetin,5-hydroxyisomucronulatol-2,5-2-O-glucoside,cholesteryl laurate,isoliquiritigenin,etc.[Conclusions]This study provides a new idea and theoretical support for future drug research and clinical practice.
基金Supported by National Natural Science Foundation of China(31200469)Natural Science Foundation of Anhui Province(1308085QC62)Training Programs of Innovation and Entrepreneurship for Undergraduates from Anhui Normal University
文摘As an important subcellular organelle for plant photosynthesis, chloroplast proteome research has become an important branch and hot issue of plant pro- teonfics. However, no efficient method has been established for extracting chloroplast proteome from plant leaves. In this paper, recent advances in extraction meth- ods of plant chloroplast proteome were summarized, which laid solid foundation for establishing efficient and practicable extraction methods of chloroplast proteome.
基金supported by the National Natural Science Foundation of China(No.82173371,82273447,82273069)the project funded by China Postdoctoral Science Foundation(No.2022M711320,2022M711322)+7 种基金Shandong Postdoctoral innovation project(China)(No.SDCX-ZG202201002)Tai Shan Young Scholar Foundation of Shandong Province,China(No.tsqn201909192)Shandong Provincial Natural Science Foundation(China)(No.ZR2020YQ59,ZR2021QH021,ZR202112020099)Youth Innovation Science and Technology Support Plan of Shandong Province’s colleges and universities(China)(No.2021KJ017)the Project of Medicine Health and Technology Development Plan of Shandong Province,China(No.202103030586 and 202103030411)the Miaopu Research of the Affiliated Hospital of Jining Medical University,Shandong,China(No.MP-ZD-2020-005 and MP-ZD-2021-001)Ph.D.Research Foundation of the Affiliated Hospital of Jining Medical University,Shandong,China(No.2022-BS003)Research Fund for Lin He’s Academician Workstation of New Medicine and Clinical Translation in Jining Medical University,Shandong,China(No.JYHL2022FZD04).
文摘Cancer stem cells(CSCs)are considered tumor-initiating cells and the main drivers of disease progression.Targeting these rare cancer cells,however,remains challenging with respect to therapeutic benefit.Here,we report the up-regulation of IL-13RA2 expression in colorectal cancer(CRC)tissues and spheroid cells.The expression of IL-13RA2 was positively correlated with canonical stemness markers in CRC.We further demonstrated that the level of IL-13 was up-regulated in the serum of CRC patients.Biologically,recombinant IL-13(rIL13)stimulation promoted the sphere formation,proliferation,and migration of CRC cells in vitro and enhanced tumorigenesis in vivo.This phenotype could be reversed by knocking down IL-13RA2.Mechanistically,IL-13 activated autophagy by inducing LC3I/LC3II transformation in CRC-CSCs,which was crucial for the biological functions of IL-13.We further demonstrated that IL-13RA2 acted as a modular link of the E3 ligase UBE3C and the substrate p53 protein,enhancing the interaction of UBE3C and p53,thereby inducing the K48-linked ubiquitination of p53.In conclusion,the IL-13/IL-13RA2 signaling cascade promotes CRC-CSC self-renewal and tumorigenesis by inducing p53 ubiquitination,adding an important layer to the connection between IL-13 and p53,which can be translated into novel targeted therapies.
基金supported by the National Natural Science Foundation of China (42001080, 42171156, and 42067049)the Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province。
文摘The carbon isotope ratios(δ^(13)C) of modern land plants have been widely studied,and they provide valuable information about modern and past atmospheric,ecological and climatic changes [1].According to their different photosynthetic pathways,land plants are mostly C_(3) and C_(4) plants.The δ^(13)C values of C_(3) plants are mainly within the range of-20‰ to-34‰.
文摘Introduction:Recent data indicate a year-on-year increase in the proportion of human immunodeficiency virus/acquired immunodeficiency syndrome(HIV/AIDS)cases reported among individuals aged≥50 in China.This study compares the epidemiological characteristics of HIV/AIDS cases in populations above and below 50 years of age.By doing so,it seeks to scrutinize the current epidemiological landscape of HIV within these distinct age cohorts and suggest tailored interventions for each group.Methods:We utilized data from the Chinese HIV/AIDS Comprehensive Response Information Management System for our comparative analysis.Joinpoint regression analysis was employed to assess the trends in standardized detection rates.Results:In China,the number of newly reported HIV/AIDS cases in the 15-49 age group increased from 51,436 in 2010 to 55,397 in 2022,while it increased from 11,751 in 2010 to 51,856 in 2022 in the group aged≥50 years.Recent years have seen a greater proportion and detection rate of HIV/AIDS cases among the≥50 age demographic compared to the 15-49 age group.In 2022,significant statistical differences were observed between males and females in both age cohorts with respect to education,marital status,occupation,mode of transmission,location of diagnosis,and region.Conclusions:The observed trend of a rising proportion of HIV/AIDS cases in individuals aged 50 years and older necessitates heightened attention.It is imperative that we develop and implement interventions specifically designed to prevent and control the transmission of HIV within this demographic.
基金supported by the Ministry of Agriculture of the People’s Republic of China(2016ZX08009-003-002)the Major Project of Science and Technology of Gansu Province(17ZD2NA016)+2 种基金the National Natural Science Foundation of China(NSFC)(31870251,31600156)the Chang Jiang Scholars Program of China(2017)Fundamental Research Funds for the Central Universities(lzujbky-2015-226)。
文摘Plant immunity must be tightly controlled to avoid activation of defense mechanisms in the absence of pathogen attack. Protein phosphorylation is a common mechanism regulating immune signaling. In Arabidopsis thaliana, nine members of the type one protein phosphatase(TOPP) family(also known as protein phosphatase 1, PP1) have been identified. Here, we characterized the autoimmune phenotype of topp4-1, a previously identified dominant-negative mutant of TOPP4. Epistasis analysis showed that defense activation in topp4-1 depended on NON-RACE-SPECIFIC DISEASE RESISTANCE1,PHYTOALEXIN DEFICIENT4, and the salicylic acid pathway.We generated topp1/4/5/6/7/8/9 septuple mutants to investigate the function of TOPPs in plant immunity. Elevated defense gene expression and enhanced resistance to Pseudomonas syringae pv. tomato(Pst) DC3000 in the septuple mutant indicate that TOPPs function in plant defense responses. Furthermore, TOPPs physically interacted with mitogen-activated protein kinases(MAPKs)and affected the MAPK-mediated downstream defense pathway. Thus, our study reveals that TOPPs are important regulators of plant immunity.
文摘Summary What is already known about this topic?Significant changes in human immunodeficiency virus(HIV)transmission modes have occurred in China,and the proportion of heterosexual transmission increased in recent years.What is added by this report?The proportions of diverse transmission routes and subgroups of heterosexual transmission were analyzed by provincial-level administrative divisions(PLADs).
文摘Summary What is already known about this topic?The annual rates of newly diagnosed cases of human immunodeficiency virus(HIV)are increasing in China,yet the annual number of newly diagnosed cases of HIV infected through injection drug use(IDU)is decreasing.
