Engineering the next generation of theranostic biomaterials with synthetic biology

Biomaterials have evolved from inert materials to responsive entities,playing a crucial role in disease diagnosis,treatment,and modeling.However,their advancement is hindered by limitations in chemical and mechanical approaches.Synthetic biology enabling the genetically reprograming of biological systems offers a new paradigm.It has achieved remarkable progresses in cell reprogramming,engineering designer cells for diverse applications.Synthetic biology also encompasses cell-free systems and rational design of biological molecules.This review focuses on the application of synthetic biology in theranostics,which boost rapid development of advanced biomaterials.We introduce key fundamental concepts of synthetic biology and highlight frontier applications thereof,aiming to explore the intersection of synthetic biology and biomaterials.This integration holds tremendous promise for advancing biomaterial engineering with programable complex functions.

重组枯草芽孢杆菌诱导表达AiiA及酶活性鉴定

[目的]由于AiiA(Autoinducer inactivation,AiiA)蛋白能有效降解AHLs(N-acyl-homoserine,AHLs),导致病原菌由于群体淬灭(Quornm quenching,QQ)失去生存优势而抑制其致病能力;利用强启动子构建能高效表达AiiA蛋白的枯草芽孢杆菌,检验枯草芽孢杆菌中Pspac启动子表达AiiA蛋白的能力,寻求有效生物防治手段防治病原菌侵害。[方法]通过已知的aiiA基因序列和启动子Pspac序列设计引物,经搭桥PCR搭连启动子Pspac和aiiA基因,构建重组表达载体Pspac-aiiA-PHY300PLK,经酶活反应确定重组菌活性。[结果]成功克隆并搭连Pspac启动子和aiiA基因,AiiA蛋白成功在重组枯草芽孢杆菌C11中表达。经酶活检验,野生菌株和重组菌株Pspac-aiiA-C11有降解信号分子AHLs的能力,但重组菌降解能力更强。[结论]重组菌Pspac-aiiA-C11较野生菌株有更强的降解AHLs效力;要利用工程菌进行生物防治还需进一步提高工程菌表达AiiA蛋白的能力及稳定性。