Objects:The aim of this study was to research the effect of grape seed procyanidin extract(GSPE) on cell apoptosis in human bladder cancer BIU87 cells and investigate its molecular mechanism.Methods:BIU87 cells were t...Objects:The aim of this study was to research the effect of grape seed procyanidin extract(GSPE) on cell apoptosis in human bladder cancer BIU87 cells and investigate its molecular mechanism.Methods:BIU87 cells were treated with different concentrations of GSPE and cultured for 24 h in vitro while untreated group as control,MTT[3-(4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide] assay,Hoechst 33258 stainning,flow cytometry,RT-PCR and Western blot were used to detect the apoptotic induction effect of GSPE on BIU87 cells.Results:We found that GSPE induced cell apoptosis in BIU87 cells by a dose-dependent manner.Semiquantitated RT-PCR and Western blot analyses indicated that GSPE increased the expression of caspase-3 and decreased the expression of survivin(P < 0.01).Conclusion:GSPE induces apoptosis in BIU87 cells in vitro,and the effect maybe related with its down-regulation of survivin.展开更多
文摘Objects:The aim of this study was to research the effect of grape seed procyanidin extract(GSPE) on cell apoptosis in human bladder cancer BIU87 cells and investigate its molecular mechanism.Methods:BIU87 cells were treated with different concentrations of GSPE and cultured for 24 h in vitro while untreated group as control,MTT[3-(4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide] assay,Hoechst 33258 stainning,flow cytometry,RT-PCR and Western blot were used to detect the apoptotic induction effect of GSPE on BIU87 cells.Results:We found that GSPE induced cell apoptosis in BIU87 cells by a dose-dependent manner.Semiquantitated RT-PCR and Western blot analyses indicated that GSPE increased the expression of caspase-3 and decreased the expression of survivin(P < 0.01).Conclusion:GSPE induces apoptosis in BIU87 cells in vitro,and the effect maybe related with its down-regulation of survivin.
