We employed our previously developed 27-plex ancestry-informative single nucleotide polymorphism(SNP)panel to infer the ancestral components of bone remains of a possible foreign pilot found in south-western China.For...We employed our previously developed 27-plex ancestry-informative single nucleotide polymorphism(SNP)panel to infer the ancestral components of bone remains of a possible foreign pilot found in south-western China.For ancestry assignment of this unknown individual,we first obtained the 27-SNP genotype of the individual.Then,based on a reference database of 3081 individuals from 33 populations,we calculated the match probability and likelihood ratio using the self-developed software program Forensic Intelligence.Inferred ancestral components of this individual were calculated by structure at K=3.A complete profile was obtained for the individual using our multiplexed SNP assay.The European population was within one order of magnitude of the highest likelihood.The major ancestral component of this individual was 97.6%European.展开更多
NESCA,a newly discovered signaling adapter protein in the NGF-pathway,contains a RUN domain at its N-terminus.Here we report the crystal structure of the NESCA RUN domain determined at 2.0-Åresolution.The overall...NESCA,a newly discovered signaling adapter protein in the NGF-pathway,contains a RUN domain at its N-terminus.Here we report the crystal structure of the NESCA RUN domain determined at 2.0-Åresolution.The overall fold of the NESCA RUN domain comprises nine helices,resembling the RUN domain of RPIPx and the RUN1 domain of Rab6IP1.However,compared to the other RUN domains,the RUN domain of NESCA has significantly different surface electrostatic distributions at the putative GTPase-interacting interface.We demonstrate that the RUN domain of NESCA can bind H-Ras,a downstream signaling molecule of TrkA,with high affinity.Moreover,NESCA RUN can directly interact with TrkA.These results provide new insights into how NESCA participates in the NGF-TrkA signaling pathway.展开更多
基金supported by the Basic Research Project[grant number 2016JB039]the Science and Technology Innovation Base Program of Beijing[grant number Z141106004414084]Key Projects in the National Science&Technology Pillar Program in the 12th-year Plan Period[grant number 2012BAK02B01].
文摘We employed our previously developed 27-plex ancestry-informative single nucleotide polymorphism(SNP)panel to infer the ancestral components of bone remains of a possible foreign pilot found in south-western China.For ancestry assignment of this unknown individual,we first obtained the 27-SNP genotype of the individual.Then,based on a reference database of 3081 individuals from 33 populations,we calculated the match probability and likelihood ratio using the self-developed software program Forensic Intelligence.Inferred ancestral components of this individual were calculated by structure at K=3.A complete profile was obtained for the individual using our multiplexed SNP assay.The European population was within one order of magnitude of the highest likelihood.The major ancestral component of this individual was 97.6%European.
基金supported financially by the National Natural Science Foundation of China(Grant Nos.31021062 and 31025009)the National Basic Research Program(973 Program)(No.2011CB910302).
文摘NESCA,a newly discovered signaling adapter protein in the NGF-pathway,contains a RUN domain at its N-terminus.Here we report the crystal structure of the NESCA RUN domain determined at 2.0-Åresolution.The overall fold of the NESCA RUN domain comprises nine helices,resembling the RUN domain of RPIPx and the RUN1 domain of Rab6IP1.However,compared to the other RUN domains,the RUN domain of NESCA has significantly different surface electrostatic distributions at the putative GTPase-interacting interface.We demonstrate that the RUN domain of NESCA can bind H-Ras,a downstream signaling molecule of TrkA,with high affinity.Moreover,NESCA RUN can directly interact with TrkA.These results provide new insights into how NESCA participates in the NGF-TrkA signaling pathway.
