Lactoferrin(LF)shows good anticancer biological activity.Lactoferrin can combine with chlorogenic acid(CGA)in dairy drinks like milk coffee and milk tea.However,the nature and functional changes of LF after binding of...Lactoferrin(LF)shows good anticancer biological activity.Lactoferrin can combine with chlorogenic acid(CGA)in dairy drinks like milk coffee and milk tea.However,the nature and functional changes of LF after binding of CGA are not clear.The stability and binding properties of CGA on LF and its antiproliferative effect on colorectal cancer SW480 cells were investigated by spectroscopic and cell experiments.The results showed that CGA could stabilize LF solution by reducing particle size(below 90 nm)and increasing zeta potential(over 14 in the absolute value).With addition of CGA,the UV absorption peak of LF solution changed(from 287 nm shifted to 290 nm)and fluorescence quenching occurred at the same time.By molecular dynamics calculation,the binding site number of CGA to LF was between 2 and 3 sites.Negative Gibbs free energy of(△G=-25.75±0.84)indicated that the interaction between LF and CGA can occur spontaneously and effectively.Cell viability and apoptosis test showed that CGA and LF inhibited the growth of colorectal cancer SW480 cells in a synergistic way.Most of cells were arrested at G0/G1 phase by the CGA-LF complex.We conclude that CGA can bind to LF to form a relatively stable complex under natural conditions,which increases their antiproliferative function on colorectal cancer cells.展开更多
Exploring efficient and economical electrocatalysts and understanding the mechanism for alkaline hydrogen oxidation reaction(HOR)are crucial to facilitate the development of alkaline polymer electrolyte fuel cells(APE...Exploring efficient and economical electrocatalysts and understanding the mechanism for alkaline hydrogen oxidation reaction(HOR)are crucial to facilitate the development of alkaline polymer electrolyte fuel cells(APEFCs).Herein,Ru_(2)P was synthesized and used as an anodic HOR electrocatalyst for APEFC,achieving a peak power density of 1.3 W cm^(−2),the highest value among Pt-free anode electrocatalysts reported under the same conditions.Fromthe density functional theory(DFT)calculations and experimental results,it was found that besides the optimized hydrogen binding energy,the enhanced adsorption strength of oxygenated species(OH*)and the reduced work function of Ru_(2)P contributed to the enhanced HOR performance.The normalized exchange current densities of Ru_(2)P/C were 0.37 mA cm_(ECSA)^(−2) and 0.27 mAμgRu^(−1),respectively,both approximately three times higher than those of Ru when conducted in the rotating disk electrode(RDE)system.Our work provides a new pathway for exploring highly active Pt-free HOR electrocatalysts and expanding the family of anodic electrocatalysts for APEFCs.展开更多
文摘Lactoferrin(LF)shows good anticancer biological activity.Lactoferrin can combine with chlorogenic acid(CGA)in dairy drinks like milk coffee and milk tea.However,the nature and functional changes of LF after binding of CGA are not clear.The stability and binding properties of CGA on LF and its antiproliferative effect on colorectal cancer SW480 cells were investigated by spectroscopic and cell experiments.The results showed that CGA could stabilize LF solution by reducing particle size(below 90 nm)and increasing zeta potential(over 14 in the absolute value).With addition of CGA,the UV absorption peak of LF solution changed(from 287 nm shifted to 290 nm)and fluorescence quenching occurred at the same time.By molecular dynamics calculation,the binding site number of CGA to LF was between 2 and 3 sites.Negative Gibbs free energy of(△G=-25.75±0.84)indicated that the interaction between LF and CGA can occur spontaneously and effectively.Cell viability and apoptosis test showed that CGA and LF inhibited the growth of colorectal cancer SW480 cells in a synergistic way.Most of cells were arrested at G0/G1 phase by the CGA-LF complex.We conclude that CGA can bind to LF to form a relatively stable complex under natural conditions,which increases their antiproliferative function on colorectal cancer cells.
基金financially supported by the National Key Research and Development Program of China(no.2018YFB1502302)the National Natural Science Foundation of China(nos.21972107,21832004,21633008,and 51872115)+1 种基金the National Natural Science Foundation of Jiangsu Province(no.BK20191186)the National Natural Science Foundation of Hubei Province(no.2020CFA095).
文摘Exploring efficient and economical electrocatalysts and understanding the mechanism for alkaline hydrogen oxidation reaction(HOR)are crucial to facilitate the development of alkaline polymer electrolyte fuel cells(APEFCs).Herein,Ru_(2)P was synthesized and used as an anodic HOR electrocatalyst for APEFC,achieving a peak power density of 1.3 W cm^(−2),the highest value among Pt-free anode electrocatalysts reported under the same conditions.Fromthe density functional theory(DFT)calculations and experimental results,it was found that besides the optimized hydrogen binding energy,the enhanced adsorption strength of oxygenated species(OH*)and the reduced work function of Ru_(2)P contributed to the enhanced HOR performance.The normalized exchange current densities of Ru_(2)P/C were 0.37 mA cm_(ECSA)^(−2) and 0.27 mAμgRu^(−1),respectively,both approximately three times higher than those of Ru when conducted in the rotating disk electrode(RDE)system.Our work provides a new pathway for exploring highly active Pt-free HOR electrocatalysts and expanding the family of anodic electrocatalysts for APEFCs.
