Growing evidences indicate that dysfunction of autophagy contributes to the disease pathogenesis of amyotrophic lateral sclerosis(ALS)and frontotemporal dementia(FTD),two neurodegenerative disorders.The GGGGCC·GG...Growing evidences indicate that dysfunction of autophagy contributes to the disease pathogenesis of amyotrophic lateral sclerosis(ALS)and frontotemporal dementia(FTD),two neurodegenerative disorders.The GGGGCC·GGCCCC repeat RNA expansion in chromosome 9 open reading frame 72(C9orf72)is the most genetic cause of both ALS and FTD.According to the previous studies,GGGGCC·GGCCCC repeat undergoes the unconventional repeat-associated non-ATG translation,which produces dipeptide repeat(DPR)proteins.Although there is a growing understanding that C9orf72 DPRs have a strong ability to harm neurons and induce C9orf72-linked ALS/FTD,whether these DPRs can affect autophagy remains unclear.In the present study,we find that poly-GR and poly-PR,two arginine-containing DPRs which display the most cytotoxic properties according to the previous studies,strongly inhibit starvation-induced autophagy.Moreover,our data indicate that arginine-rich DPRs enhance the interaction between BCL2 and BECN1/Beclin 1 by inhibiting BCL2 phosphorylation,therefore they can impair autophagic clearance of neurodegenerative disease-associated protein aggregates under starvation condition in cells.Importantly,our study not only highlights the role of C9orf72 DPR in autophagy dysfunction,but also provides novel insight that pharmacological intervention of autophagy using SW063058,a small molecule compound that can disrupt the interaction between BECN1 and BCL2,may reduce C9orf72 DPR-induced neurotoxicity.展开更多
Gefitinib,a well-known epidermal growth factor receptor(EGFR)tyrosine kinase inhibitor for the targeted therapy of lung cancer,induces autophagy in association with drug resistance.However,it remains unclear whether g...Gefitinib,a well-known epidermal growth factor receptor(EGFR)tyrosine kinase inhibitor for the targeted therapy of lung cancer,induces autophagy in association with drug resistance.However,it remains unclear whether gefitinib treatment can affect the selective form of autophagy(i.e.,mitophagy)and be beneficial for the treatment of human diseases with decreased autophagy,such as neurodegenerative diseases.Here,we show that gefitinib treatment promotes PINK1/Parkin-mediated mitophagy in both nonneuronal and neuronal cells,and this effect is independent of EGFR.Moreover,we found that gefitinib treatment increases the recruitment of the autophagy receptor optineurin(OPTN)to damaged mitochondria,which is a downstream signaling event in PINK1/Parkin-mediated mitophagy.In addition,gefitinib treatment significantly alleviated neuronal damage in TBK1-deficient neurons,resulting in impeded mitophagy.In conclusion,our study suggests that gefitinib promotes PINK1/Parkin-mediated mitophagy via OPTN and may be beneficial for the treatment of neurodegenerative diseases that are associated with defective mitophagy.展开更多
基金This work was supported by the National Natural Science Foundation of China(Nos.82022022,32371018 and 82071274)a Project Funded by Jiangsu Key Laboratory of Neuropsychiatric Diseases(BM2013003,China)+4 种基金a Key Project of Natural Science Foundation of Jiangsu Provincial Higher Education Institutions(23KJA310005,China)a Project Funded by the Interdisciplinary Basic Frontier Innovation Program of Suzhou Medical College of Soochow University(MP13202823,China)a Project Funded by the Suzhou International Joint Laboratory for Diagnosis and Treatment of Brain Diseases,and a Project Funded by the Priority Academic Program Development of the Jiangsu Higher Education Institutes(PAPD).J.H.M.P.was supported Science Foundation Ireland(17/COEN/3474,17/JPND/3455)Q.M.is a recipient of an RCSI International StAR Ph.D.scholarship.N.L.was supported by the Postgraduate Research&Practice Innovation Program of Jiangsu Province.K.Y.T.,was supported by the financial support from the Science and Technology Development Fund,Macao SAR(File no.0062/2021/A,China)University of Macao(File no.MYRG2022-00171-FHS,China).
文摘Growing evidences indicate that dysfunction of autophagy contributes to the disease pathogenesis of amyotrophic lateral sclerosis(ALS)and frontotemporal dementia(FTD),two neurodegenerative disorders.The GGGGCC·GGCCCC repeat RNA expansion in chromosome 9 open reading frame 72(C9orf72)is the most genetic cause of both ALS and FTD.According to the previous studies,GGGGCC·GGCCCC repeat undergoes the unconventional repeat-associated non-ATG translation,which produces dipeptide repeat(DPR)proteins.Although there is a growing understanding that C9orf72 DPRs have a strong ability to harm neurons and induce C9orf72-linked ALS/FTD,whether these DPRs can affect autophagy remains unclear.In the present study,we find that poly-GR and poly-PR,two arginine-containing DPRs which display the most cytotoxic properties according to the previous studies,strongly inhibit starvation-induced autophagy.Moreover,our data indicate that arginine-rich DPRs enhance the interaction between BCL2 and BECN1/Beclin 1 by inhibiting BCL2 phosphorylation,therefore they can impair autophagic clearance of neurodegenerative disease-associated protein aggregates under starvation condition in cells.Importantly,our study not only highlights the role of C9orf72 DPR in autophagy dysfunction,but also provides novel insight that pharmacological intervention of autophagy using SW063058,a small molecule compound that can disrupt the interaction between BECN1 and BCL2,may reduce C9orf72 DPR-induced neurotoxicity.
基金supported by the National Natural Science Foundation of China(Grant Nos.82022022,31771117,and 82071274)a Project Funded by the Suzhou Science and Technology Program(Grant No.SYS2019068)+2 种基金a Project Funded by the Clinical Research Program of the WuJieping Medical Foundation(Grant No.320.6750.19092-32)a Project Funded by Jiangsu Key Laboratory of Neuropsychiatric Diseases(Grant No.BM2013003)a Project Funded by the Priority Academic Program Development of the Jiangsu Higher Education Institutes(PAPD).
文摘Gefitinib,a well-known epidermal growth factor receptor(EGFR)tyrosine kinase inhibitor for the targeted therapy of lung cancer,induces autophagy in association with drug resistance.However,it remains unclear whether gefitinib treatment can affect the selective form of autophagy(i.e.,mitophagy)and be beneficial for the treatment of human diseases with decreased autophagy,such as neurodegenerative diseases.Here,we show that gefitinib treatment promotes PINK1/Parkin-mediated mitophagy in both nonneuronal and neuronal cells,and this effect is independent of EGFR.Moreover,we found that gefitinib treatment increases the recruitment of the autophagy receptor optineurin(OPTN)to damaged mitochondria,which is a downstream signaling event in PINK1/Parkin-mediated mitophagy.In addition,gefitinib treatment significantly alleviated neuronal damage in TBK1-deficient neurons,resulting in impeded mitophagy.In conclusion,our study suggests that gefitinib promotes PINK1/Parkin-mediated mitophagy via OPTN and may be beneficial for the treatment of neurodegenerative diseases that are associated with defective mitophagy.