Dear Editor,Coxsackievirus A16(CA16)and enterovirus A71(EV-A71)are the main causative agents of hand foot and mouth disease(HFMD)(Alexander et al.,1994).Besides HFMD,EV-A71 infection in young children can lead to a sp...Dear Editor,Coxsackievirus A16(CA16)and enterovirus A71(EV-A71)are the main causative agents of hand foot and mouth disease(HFMD)(Alexander et al.,1994).Besides HFMD,EV-A71 infection in young children can lead to a spectrum of other clinical diseases and associated neurological complications and mortality(Melnick JL.,1996).In recent decades,EV-A71 infections have become a major public health concern throughout the world,following the frequent occurrence of epidemics and outbreaks of HFMD associated with neurological complications and high mortality in young children(Ishimaru et al.,1980;Tagaya et al.,1981;Chomnaitree et al.,1982;Samuda et al.,1987;Gilbert et al.,1988;Hayward et al.,1989;Lum et al.,1998;Chang et al.,1998;Liu et al.,2000;Yang et al.,2011;Zeng et al.,2012).展开更多
Dear Editor,Previous studies had described the adaptation of enterovirus 71(EV-A71)strains that enabled entry and viral replication in Chinese Hamster Ovary(CHO)cell line(Zaini and McMinn 2012;Zaini et al.2012).These ...Dear Editor,Previous studies had described the adaptation of enterovirus 71(EV-A71)strains that enabled entry and viral replication in Chinese Hamster Ovary(CHO)cell line(Zaini and McMinn 2012;Zaini et al.2012).These adapted strains derived from serial passage of a clinical isolate in CHO cells exhibited an amino acid substitution at VP2149,which enhanced viral replication by 100*1000-fold compared to the clinical isolate.The VP2149 mutation was claimed responsible for adaptation to CHO-K1 cells without performing detailed molecular analyses to support these claims.In this study,we evaluate various VP1 and VP2 mutations in two CHO-adapted EV-A71 strains derived in our lab to assess their contribution to the phenotype of CHO cell adaptation.展开更多
基金funded by the Temasek Lifesciences Laboratory core funding
文摘Dear Editor,Coxsackievirus A16(CA16)and enterovirus A71(EV-A71)are the main causative agents of hand foot and mouth disease(HFMD)(Alexander et al.,1994).Besides HFMD,EV-A71 infection in young children can lead to a spectrum of other clinical diseases and associated neurological complications and mortality(Melnick JL.,1996).In recent decades,EV-A71 infections have become a major public health concern throughout the world,following the frequent occurrence of epidemics and outbreaks of HFMD associated with neurological complications and high mortality in young children(Ishimaru et al.,1980;Tagaya et al.,1981;Chomnaitree et al.,1982;Samuda et al.,1987;Gilbert et al.,1988;Hayward et al.,1989;Lum et al.,1998;Chang et al.,1998;Liu et al.,2000;Yang et al.,2011;Zeng et al.,2012).
基金fully funded by Temasek Lifesciences Laboratory Ltd.
文摘Dear Editor,Previous studies had described the adaptation of enterovirus 71(EV-A71)strains that enabled entry and viral replication in Chinese Hamster Ovary(CHO)cell line(Zaini and McMinn 2012;Zaini et al.2012).These adapted strains derived from serial passage of a clinical isolate in CHO cells exhibited an amino acid substitution at VP2149,which enhanced viral replication by 100*1000-fold compared to the clinical isolate.The VP2149 mutation was claimed responsible for adaptation to CHO-K1 cells without performing detailed molecular analyses to support these claims.In this study,we evaluate various VP1 and VP2 mutations in two CHO-adapted EV-A71 strains derived in our lab to assess their contribution to the phenotype of CHO cell adaptation.
