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简化基因组测序评价高邮鸭保种效果的研究 被引量:4
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作者 刘宏祥 宋卫涛 +5 位作者 薛敏开 秦豪荣 朱春红 陶志云 徐文娟 李慧芳 《中国家禽》 北大核心 2018年第22期11-15,共5页
研究旨在评价高邮鸭原种场的保种效果,为高邮鸭保种提供理论依据。利用简化基因组测序方法ddRAD鉴定高邮鸭原始群体(GS)和原种场群体(GC)的SNP标记,比较分析两个群体的遗传多样性差异,并使用遗传分化指数(F_(ST))和核苷酸多样性比值(π ... 研究旨在评价高邮鸭原种场的保种效果,为高邮鸭保种提供理论依据。利用简化基因组测序方法ddRAD鉴定高邮鸭原始群体(GS)和原种场群体(GC)的SNP标记,比较分析两个群体的遗传多样性差异,并使用遗传分化指数(F_(ST))和核苷酸多样性比值(π ratio)比率联合筛选选择信号区域及其相关基因,对得到的受选基因进行GO和KEGG功能富集分析。结果显示:与原始群体相比,原种场群体遗传杂合度保持不变,近交系数有所下降;原种场群体与环境应激和甲基化调控相关的基因受到了一定程度的选择。结果表明高邮鸭原种场群体保种效果较好,可以继续沿用现有的小群保种方法。 展开更多
关键词 高邮鸭 保种 简化基因组测序
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基于简化基因组测序评价太湖鹅不同保种场的保种效果 被引量:3
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作者 刘宏祥 朱满兴 +6 位作者 秦豪荣 宋卫涛 朱春红 陶志云 徐文娟 章双杰 李慧芳 《华北农学报》 CSCD 北大核心 2018年第A01期94-99,共6页
旨在评价太湖鹅原种场和国家水禽基因库的保种效果,为进一步更好地对太湖鹅进行保种打下理论依据。利用ddRAD简化基因组测序方法鉴定太湖鹅原始群体(TS)、原种场群体(TC)和基因库群体(TK)的SNP标记,比较分析3个群体的遗传多样性差异,并... 旨在评价太湖鹅原种场和国家水禽基因库的保种效果,为进一步更好地对太湖鹅进行保种打下理论依据。利用ddRAD简化基因组测序方法鉴定太湖鹅原始群体(TS)、原种场群体(TC)和基因库群体(TK)的SNP标记,比较分析3个群体的遗传多样性差异,并使用遗传分化系数FST和核苷酸多样性π比值联合筛选选择信号区域及其相关基因,对得到的受选基因进行GO和KEGG功能富集分析。结果发现,与原始群体相比,原种场群体和基因库群体的遗传杂合度均有降低,近交系数上升,且基因库群体与原始群体、原种场群体有中度分化的趋势。原种场群体的脂类代谢相关基因受到了一定程度的选择。结果表明,原种场群体和基因库群体应适当增加保种群数量,避免近交系数上升过快,且基因库群体应加强与原种场群体的基因交流,避免与原始群体、原种场群体的过度分化。 展开更多
关键词 保种 简化基因组测序
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Incubation Effect on Eggs of Tibetan Chicken under Low Altitude Condition
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作者 Zhang Ling Jiang Chunmao +3 位作者 Duan Xiujun Yang Xiaozhi qin haorong Liu Dan 《Animal Husbandry and Feed Science》 CAS 2017年第4期206-208,212,共4页
[ Objective] In order to study incubation conditions of Tibetan chickens in low altitude areas. [ Method] Eggs of Tibetan chicken were transported to low altitude areas in highland areas for artificial hatching. [ Res... [ Objective] In order to study incubation conditions of Tibetan chickens in low altitude areas. [ Method] Eggs of Tibetan chicken were transported to low altitude areas in highland areas for artificial hatching. [ Results] The results showed that the average hatching rate of fertilized eggs was 85.33% ; the average healthy chick rate was 91.77 % ; the average primary body mass was 31.2l g. Hatching rate of eggs in group 2 was the highest, which reached 90.52%, the aver- age egg mass was 43.25 g and the average egg shape index was 1.32. Hatching rate of eggs in group 2 was 13.11% and 2.47% higher than that in group 1 and group 3 respectively, which was 5.19% higher than the average value. Hatching rate and healthy chick rate of eggs in group 2 also showed the same trend. [ Conclusion] High hatching effect could be obtained under the better hatching condition in low altitude areas in highland areas. 展开更多
关键词 Tibetan chicken Hatching egg Incubation effect Low elevation
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Appropriate Metabolic Energy, Crude Protein, Calciumand Available Phosphorus Levels of Diet for BlackMuscovy Duck at 43-91 Days of Age
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作者 Zhang Ling Gu Wenjie +4 位作者 Zhang Kai Duan Xiujun qin haorong Bian Youqing duan yongbo 《Animal Husbandry and Feed Science》 CAS 2018年第4期257-263,共7页
[Objective] The paper was to study the effects of diet formulation with different nutrition levels on growth and slaughter performance ofblack muscovy duck at the age of 43-91 days. [Method] Totally 288 individuals of... [Objective] The paper was to study the effects of diet formulation with different nutrition levels on growth and slaughter performance ofblack muscovy duck at the age of 43-91 days. [Method] Totally 288 individuals of 43dayold black muscovy duck were randomly divided intonine treatments, four repeats each treatment and eight ducks each repeat. UsingL9 (34) orthogonal experiment design (four factors and three levels),three metabolic energy levels (12.32, 12.72, 13.12 MJ/kg), three crude protein levels (14%, 16%, 18%), three calcium levels (0.6%, 0.8%, 1.0%)and three available phosphorus levels (0.30%, 0.45%, 0.6%) were designed, and a total of nine feeding groups (C1C9) were designed according todifferent nutritional levels. Through combination of feeding test (49 d) and metabolic test (pretest 4 d and formal test 3 d), the growth performance,meat performance and apparent nutrient digestibility of ducks were determined. [Result] The ducks in experimental group C2 had the highest average daily gain, the lowest feed gain ratio, the largest body oblique length, chest width, chest depth and tibia length, which had significant or extremely significant differences with other groups (P〈0.05 or P〈0.01). The slaughter rate, semieviscerated rate, eviscerated rate and meat quality ofducks in C2 group were higher (P〈0.05). The apparent digestibility of crude protein and dietary metabolic energy in C2 group were 88.68% and63.07%, respectively, both were significantly or extremely significantly higher than the other groups(P〈0.05 or P〈0.01), while the interaction effectof calcium and phosphorus level on apparent digestibility was not obvious. [Conclusion] Under the experimental conditions, the suitable metabolicenergy, crude protein, calcium, available phosphorus and total phosphorus levels of 43 -91 days old black muscovy ducks were 12.32 MJ/kg,16.10%, 0.92%, 0.45% and 0.68%, respectively. 展开更多
关键词 Black muscovy duck Nutrition level Growth performance Meat performance Apparent digestibility
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基于灰关联和主成分分析的6个中国白鹅品种综合评价 被引量:1
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作者 秦豪荣 李晓鸣 +3 位作者 杨晓志 孙国波 王健 董飚 《黑龙江畜牧兽医》 CAS 北大核心 2023年第7期60-65,共6页
为了探讨灰关联分析和主成分分析在水禽资源评价方面的应用效果,试验对6个中国白鹅品种的体重、体尺、屠宰性能、繁殖性能等共18个性状指标分别进行了灰关联分析和主成分分析,同时采用欧式距离法对6个品种进行聚类分析。结果表明:在灰... 为了探讨灰关联分析和主成分分析在水禽资源评价方面的应用效果,试验对6个中国白鹅品种的体重、体尺、屠宰性能、繁殖性能等共18个性状指标分别进行了灰关联分析和主成分分析,同时采用欧式距离法对6个品种进行聚类分析。结果表明:在灰关联分析中,按照平均关联度从高到低依次为浙东白鹅(0.723)、皖西白鹅(0.708)、武冈铜鹅(0.667)、四川白鹅(0.530)、太湖鹅(0.506)、豁眼鹅(0.480);在主成分分析中,利用隶属函数计算的主成分综合值从高到低依次为武冈铜鹅(0.743)、浙东白鹅(0.690)、皖西白鹅(0.623)、太湖鹅(0.325)、四川白鹅(0.311)、豁眼鹅(0.248);6个白鹅品种分为两类,四川白鹅、太湖鹅、豁眼鹅聚为一类,浙东白鹅、武冈铜鹅、皖西白鹅聚为一类,分类符合品种的种质特性。说明灰关联分析和主成分分析是综合运用多项指标评价水禽品种资源的一种有效方法。 展开更多
关键词 灰关联分析 主成分分析 中国白鹅 品种资源 综合评价
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不同地方品种鹅ALV p27抗原的检测
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作者 穆晓惠 肖文华 +2 位作者 李晓鸣 张干生 秦豪荣 《黑龙江畜牧兽医》 CAS 北大核心 2019年第6期67-69,共3页
为了解鹅禽白血病病毒(ALV)的自然感染状态,试验采集4种不同地方品种鹅(豁眼鹅、广丰白翎鹅、浙东白鹅、武岗鹅)、3个时期(6周龄、22周龄和37周龄)的泄殖腔棉拭子和对应的血清样品各1 089份,同时采集产蛋期蛋清样品共180份,利用ALV抗原... 为了解鹅禽白血病病毒(ALV)的自然感染状态,试验采集4种不同地方品种鹅(豁眼鹅、广丰白翎鹅、浙东白鹅、武岗鹅)、3个时期(6周龄、22周龄和37周龄)的泄殖腔棉拭子和对应的血清样品各1 089份,同时采集产蛋期蛋清样品共180份,利用ALV抗原检测试剂盒进行检测。结果表明:每个品种鹅的不同时期样品均能够检测到ALV p27抗原,其中血清样品阳性率相对较高(6. 5%~8. 8%),泄殖腔棉拭子样品阳性率显著低于血清样品(P<0. 05),蛋清样品阳性率最低(0~2. 2%);不同品种同一样品间ALV p27抗原阳性率差异不显著(P>0. 05)。说明在鹅中可以检测到ALV p27抗原,且血清样品检测阳性率高于泄殖腔棉拭子和蛋清样品,但ALV在鹅中没有表现出品种差异性。 展开更多
关键词 禽白血病病毒 P27抗原 ELISA 蛋清 血清
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