拟建四川昭觉地质公园地质遗迹景观资源特征

拟建四川昭觉地质公园地质遗迹景观资源较为丰富,类型多样,主要沿省道S307、S208呈带状分布,计有7大类15类17亚类84处地质遗迹景观,最具特色的是央摩租乡小龙足迹,其大小不足3cm,全球只发现于四川峨眉、山东莒南、韩国南海岸,是国内第三例、世界第四例最小的兽脚类足迹,并且央摩租的小龙足迹与大型足迹共存还是世界上首次记录,对研究恐龙足迹组合,恐龙古行为学都有重大的意义。

CT及X线在股骨头坏死分期诊断中的价值评价

目的:评价CT及X线在股骨头坏死分期中的诊断准确性及价值。方法:收集我院2016年6月-2018年6月收治的46例确诊为股骨头坏死患者的影像资料,所有患者均进行CT及X线检查,行CT检查的为CT组,行X线检查的为X线组。比较两组患者股骨头坏死的阳性检出率及0期、I期、II期、III期、IV期分期诊断检出率。结果:CT组患者的阳性检出率(82.6%)显著高于X线组(63.0%),二者差异有统计学意义(Χ2=4.449,P<0.05);且CT组患者在0期、I期、II期股骨头坏死检出率高于X线组(均P<0.05),而在III期、IV期的检出率,二者差异无统计学意义(均P>0.05)。结论:CT对股骨头坏死的检出率较X线高,且在股骨头坏死的早期诊断中较占优势。

Simultaneous Determination of Trace Rare Earth Elements and Other Elements in High Purity Terbium Oxide (Tb_4O_7) by ICP-AES After HPLC Separation Using P507 Resin

This article describes a new method for the simultaneous determination of trace rare earth elements (REEs) and non rare earth elements (NREEs) in high purity terbium oxide by ICP-AES after HPLC separation using P507 resin. The chromatographic separation of the analytes from the matrix using dilute nitric acid as mobile phase was studied. The experimental results showed that a favorable separation of trace metals (Cu and Gd) from the matrix (Tb) can easily be achieved by elution with dilute nitric acid within 25 min. The proposed method was applied to the determination of trace metals (Ca, Cu, Mg, Mn, Ni, Si, La, Ce, Pr, Nd, Sm, Eu and Gd) in high purity terbium oxide. The detection limits (DLs) for the analytes ranged from 0.4-4.0 μg\5g -1, and the recoveries are from 78%-105%.

基于AHP的大学校园快递点服务质量模糊综合评价

为了客观、有效地评价大学校园快递点的服务质量,文中采用层次分析法选取服务态度、服务效率、收发快递的方便程度和快递完好程度四个一级评价指标,并把一级指标进行再划分,从而与模糊综合评价相结合来建立校园快递服务质量的评价模型,并选取A、B、C、D四家快递服务点进行服务质量的评价,最后对评价结果提出相关建议。

Study on the Flow Injection Micro-Column Pre-Separation System Coupled With High Performance Liquid Chromatography for the Determination of Ecdysterone in Traditional Chinese Medicine

A flow injection (FI) micro-column system coupled with high performance liquid chromatography (HPLC) was proposed for the pre-separation and determination of active organic component (ecdysterone) in traditional Chinese medicine, Loulu. The factors influencing separation performance were investigated and optimized. Under the optimal conditions, the contents of ecdysterone in Loulu were determined by HPLC system using MeOH-H_2O (40: 60,V/V) as the mobile phase at a flow rate of 1. 0 mL/min. The calibration curve was linear in the range of 0. 5~ 100 mg/L of ecdysterone concentrations. The detection limit of the analyte was 0. 11mol/L(3) with a precision of 0. 38% RSD (n=7 f c= 10. 0 mg/L). The average recovery of the method was 98. 7%. The proposed method has been applied to determine ecdysterone in practical samples, and the determined values by both external standard method and standard addition method were in good agreement. Compared to the traditional solid extraction method, the system proposed has the advantages of simple procedure, good reproducibility, minimum volume requirement, reduction of matrix interference and low contamination risk.

下调宫颈癌细胞FAM111B表达对增殖、周期和凋亡的影响

目的观察下调FAM111B对宫颈癌细胞系C-33A和HeLa增殖、细胞周期和凋亡的影响及其可能机制。方法应用慢病毒载体siRNA-FAM111B及Null,分别感染C-33A和HeLa细胞。实时定量PCR方法(qRTPCR)和Western blot方法分别检查各组细胞FAM111B基因与蛋白的表达。应用CCK-8方法检测FAM111B对细胞增殖能力的影响。流式细胞术PI染色细胞周期检测各组细胞周期变化。流式细胞术Annexin V/PI双染方法检测各组细胞的凋亡情况。Western blot法检测p53及下游Bax和Bcl-2的表达。结果成功转染C-33A和HeLa细胞,FAM111B基因与蛋白表达水平均下调。转染siR-FAM111B能抑制宫颈癌细胞的增殖,C-33A和HeLa细胞发生G1期阻滞。下调FAM111B诱导宫颈癌细胞凋亡,促进p53蛋白表达,进而上调Bax蛋白和下调Bcl-2蛋白的表达。结论下调FAM111B抑制宫颈癌细胞的增殖,促使细胞周期G1期阻滞,诱导细胞凋亡,与调节p53蛋白进而激活下游相关信号通路相关。

脐血间充质干细胞对同基因及异基因T淋巴细胞亚群的影响

目的探讨脐血间充质干细胞对同基因和异基因来源的T淋巴细胞亚群数量的影响。方法取新生儿脐血,体外培养间充质干细胞和T淋巴细胞,间充质干细胞和同基因及异基因T淋巴细胞共培养,流式细胞仪分析其亚群细胞的数量变化。结果与间充质干细胞共培养的T淋巴细胞中CD3^(+)CD4^(+)T淋巴细胞、CD3^(+)CD8^(+)T淋巴细胞及CD4^(+)CD25^(+)调节性T细胞的数量均增多,且异基因来源的CD3^(+)C D4^(+)T淋巴细胞的数量与同基因来源的CD3^(+)CD4^(+)T淋巴细胞的数量相比,数量增多更显著(P<0.05)。结论脐血间充质干细胞可以促进同基因和异基因T淋巴细胞的增殖,为间充质干细胞应用于临床疾病的治疗提供了数据支持。