Aim:To investigate the effect of Trx-LHRH, a new GnRH crasis protein, on antibody production and reproductive function in male rats. Methods: Trx-LHRH produced in vitro with a new crasis gene, which crasised Trx gene ...Aim:To investigate the effect of Trx-LHRH, a new GnRH crasis protein, on antibody production and reproductive function in male rats. Methods: Trx-LHRH produced in vitro with a new crasis gene, which crasised Trx gene and GnRH gene together, was used as a vaccine with hydroalaminum base as the adjuvant in adult male SD rats. A second inoculation was performed 5 weeks later. Antibody level was measured by ELISA and androgen level by RIA thereafter. Results: Trx-LHRH successfully induced polycolonal antibody at the level of 1:1 280 - 2 560 four weeks after the first inoculation and 1:2 000 SIX weeks after the enhanced treatment. Testosterone level was reduced significantly (P<0.01). Sperm count was also reduced by Trx-LHRH treatment. However, there was reasonable individual variations. Conclusion: Trx-LHRH can be used as an effective vaccine to induce antibody production; at the same time, it restrains the function of hypothatamas-pituitary-testis axis.展开更多
文摘Aim:To investigate the effect of Trx-LHRH, a new GnRH crasis protein, on antibody production and reproductive function in male rats. Methods: Trx-LHRH produced in vitro with a new crasis gene, which crasised Trx gene and GnRH gene together, was used as a vaccine with hydroalaminum base as the adjuvant in adult male SD rats. A second inoculation was performed 5 weeks later. Antibody level was measured by ELISA and androgen level by RIA thereafter. Results: Trx-LHRH successfully induced polycolonal antibody at the level of 1:1 280 - 2 560 four weeks after the first inoculation and 1:2 000 SIX weeks after the enhanced treatment. Testosterone level was reduced significantly (P<0.01). Sperm count was also reduced by Trx-LHRH treatment. However, there was reasonable individual variations. Conclusion: Trx-LHRH can be used as an effective vaccine to induce antibody production; at the same time, it restrains the function of hypothatamas-pituitary-testis axis.
