SLC6A14 promotes ulcerative colitis progression by facilitating NLRP3 inflammasome-mediated pyroptosis

BACKGROUND Ulcerative colitis(UC)is an inflammatory condition with frequent relapse and recurrence.Evidence suggests the involvement of SLC6A14 in UC pathogenesis,but the central regulator remains unknown.AIM To explore the role of SLC6A14 in UC-associated pyroptosis.METHODS Quantitative real-time polymerase chain reaction(qRT-PCR),immunoblotting,and immunohistochemical were used to assess SLC6A14 in human UC tissues.Lipopolysaccharide(LPS)was used to induce inflammation in FHC and NCM460 cells and model enteritis,and SLC6A14 levels were assessed.Pyroptosis markers were quantified using enzyme-linked immunosorbent assay,Western blotting,and qRT-PCR,and EdU incubation,CCK-8 assays and flow cytometry were used to examine proliferation and apoptosis.Mouse models of UC were used for verification.RESULTS SLC6A14 was increased and correlated with NLRP3 in UC tissues.LPS-induced FHC and NCM460 cells showed increased SLC6A14 levels.Reducing SLC6A14 increased cell proliferation and suppressed apoptosis.Reducing SLC6A14 decreased pyroptosis-associated proteins(ASC,IL-1β,IL-18,NLRP3).NLRP3 overexpression counteracted the effects of sh-SLC6A14 on LPS-induced FHC and NCM460 cell pyroptosis.SLC6A14 improved the mucosa in mice with dextran sulfate sodium-induced colitis.CONCLUSION SLC6A14 promotes UC pyroptosis by regulating NLRP3,suggesting the therapeutic potential of modulating the SLC6A14/NLRP3 axis.

基于LSTM常模式阵列的燃气轮机高温部件异常检测方法

燃气轮机高温部件的故障发生率高、隐蔽性强、破坏性大,且故障发生后维修成本高、维修难度大。研究一种异常检测方法,在高温部件故障早期及时发现部件异常并进行检修意义重大。本文介绍了一种基于LSTM常模式阵列异常检测方法,利用常模式思想提取出反映燃气轮机高温部件性能状态的特征映射,通过LSTM特有的处理时间序列数据的能力,建立LSTM常模式阵列异常检测方法实现高温部件故障早期异常状态灵敏识别。在实际数据上进行方法有效性验证,并与西门子的基于排温保护逻辑的异常检测方法对比,证明了基于LSTM常模式阵列异常检测方法的灵敏性和有效性。

Transgelin as a potential target in the reversibility of pulmonary arterial hypertension second to congenital heart disease

Background The reversibility of pulmonary arterial hypertension(PAH)in congenital heart disease(CHD)is of great importance for the operability of CHD.Proteomics analysis found that transgelin was significantly upregulated in the lung tissue of CHD-PAH patients,especially in the irreversible group.However,how exactly it participated in CHD-PAH development is unknown.

Establishing an experimental rat model of photo-dynamically-induced retinal vein occlusion using erythrosin B

AIM:To develop a reliable,reproducible rat model of retinal vein occlusion(RVO)with a novel photosensitizer(erythrosin B)and study the cellular responses in the retina.METHODS:Central and branch RVOs were created in adult male rats via photochemically-induced ischemia.Retinal changes were monitored via color fundus photography and fluorescein angiography at 1 and 3h,and 1,4,7,14,and 21d after irradiation.Tissue slices were evaluated histopathologically.Retinal ganglion cell survival at different times after RVO induction was quantified by nuclear density count.Retinal thickness was also observed.RESULTS:For all rats in both the central and branch RVO groups,blood flow ceased immediately after laser irradiation and retinal edema was evident at one hour.The retinal detachment rate was 100%at 3h and developed into bullous retinal detachment within 24h.Retinal hemorrhages were not observed until 24h.Clearance of the occluded veins at 7d was observed by fluorescein angiography.Disease manifestation in the central RVO eyes was more severe than in the branch RVO group.A remarkable reduction in the ganglion cell count and retinal thickness was observed in the central RVO group by 21d,whereas moderate changes occurred in the branch RVO group.CONCLUSION:Rat RVO created by photochemicallyinduced ischemia using erythrosin B is a reproducible and reliable animal model for mimicking the key features of human RVO.However,considering the 100%rate ofretinal detachment,this animal model is more suitable for studying RVO with chronic retinal detachment.

Subhalo Abundance and Satellite Spatial Distribution in Milky Way-Andromeda-like Paired Haloes

We study the subhalo and satellite populations in haloes similar to the Milky Way(MW)-Andromeda paired configuration in the Millennium II and P-Millennium simulations.We find subhaloes are 5%–15%more abundant in paired haloes than their isolated counterparts that have the same halo mass and large-scale environmental density.Paired haloes tend to reside in a more isotropic environment than isolated haloes,the shear tensor of their large-scale tidal field is possibly responsible for this difference.We also study the thickness of the spatial distribution of the top 11 most massive satellite galaxies obtained in the semi-analytic galaxy sample constructed from the Millennium II simulation.Moreover,satellites that have lost their host subhaloes due to the resolution limit of the simulation have been taken into account.As a result,we find that the difference in the distribution of the satellite thickness between isolated and paired haloes is indistinguishable,which suggests that the paired configuration is not responsible for the observed plane of satellites in the MW.The results in this study indicate the paired configuration could bring some nonnegligible effect on the subhalo abundance in the investigation of the MW's satellite problems.

Significance of the left ventricular ejection/filling parameters in patients with pulmonary arterial hypertension:a magnetic resonance imaging based study

Background The goal of this study was to explore the significance of left ventricular(LV)ejection/filling parameters in assessing severity and prognosis of pulmonary arterial hypertension(PAH).Methods Newly diagnosed PAH patients were recruited between July 2011 and December 2013 prospectively.Cardiopulmonary hemodynamics derived from right heart catheterization,and LV ejection/filling parameters derived from CMR were collected.Follow-up data were collected periodically using a specifically-designed network database until April 30,2016.

The clinical features and effects with target therapy for post splenectomy pulmonary hypertension

Objective To analyze the clinical features and effects of target therapy of post splenectomy pulmonary hypertension, and improve the diagnosis and treatment of the disease.Methods Clinical data of 18 patients with post splenectomy pulmonary hypertension admitted to our hospital from October 2006 to March 2017 were systematically reviewed.

Sodium dichloroacetate could inhibit the endothelial-to-mesenchymal transition:a novel treatment target for pulmonary arterial hypertension

Background and Purpose Recent studies found endothelialto-mesenchymal transition(EndoMT)played an important role in the pathogenesis of pulmonary arterial hypertension.Our pilot research demonstrated the existence of Warburg effect in the lung tissue of idiopathic pulmonary arterial hypertension patients.However the relationships and the underlying mechanisms between EndoMT and Warburg effect have not been elucidated.Therefore,the purpose of this study is to determine whether metabolic reprogramming happens in EndoMT cells.We also want to investigate whether sodium dichloroacetate(DCA),a metabolic modulator,could prevent EndoMT by inhibiting Warburg effect.

The Halo Concentration and Mass Relation Traced by Satellite Galaxies

We study the relation between halo concentration and mass(c-M relation) using galaxy catalogs of the Seventh and Eighth Data Releases of the Sloan Digital Sky Survey(SDSS DR7 and DR8). Assuming that the satellite galaxies follow the distribution of dark matter, we derive the halo concentration by fitting the satellite radial profile with a Nararro Frank and White(NFW) format. The derived c-M relation covers a wide halo mass range from10^(11.6)to 10^(14.1)M_(⊙). We confirm the anti-correlation between the halo mass and concentration as predicted in cosmological simulations. Our results are in good agreement with those derived using galaxy dynamics and gravitational lensing for halos of 10^(11.6)– 10^(12.9)M_(⊙), while they are slightly lower for halos of 10^(12.9)– 10^(14.1)M_(⊙).This is because blue satellite galaxies are less concentrated, especially in the inner regions. Instead of using all satellite galaxies, red satellites could be better tracers of the underlying dark matter distribution in galaxy groups.

Unexplained septic shock after colonoscopy with polyethylene glycol preparation in a young adult:A case report

BACKGROUND Colonoscopy has become a routine physical examination as people’s health awareness has increased.Polyethylene glycol(PEG)is greatly used in bowel preparation before colonoscopy due to its price and safety advantages.Septic shock after colonoscopy with PEG preparation is extremely rare,with only very few cases in critically ill patients.Herein,we describe a case of septic shock in a healthy young adult immediately following colonoscopy with PEG preparation.CASE SUMMARY A 33-year-old young adult presented to our hospital for colonoscopy with PEG bowel preparation due to recurrent diarrhea for 7 years.The male's previous physical examination showed no abnormal indicators,and colonoscopy results were normal;however,he exhibited septic shock and markedly elevated white blood cell,C-reactive protein,and procalcitonin levels on the second day after colonoscopy.Immediate resuscitation and intensive care with appropriate antibiotics improved his condition.However,the blood and stool cultures did not detect the pathogen CONCLUSION Septic shock after colonoscopy is rare,especially in young adults.The authors considered the possibility of opportunistic infections after PEG bowel preparation,and clinicians should monitor patients for the possibility of such complications.

手性环戊二烯配体及其金属配合物的合成研究进展

环戊二烯基负离子能与金属形成稳定的配合物,在金属催化反应中有着广泛的应用.随着不对称催化反应的快速发展,一系列具有不同骨架的手性环戊二烯配体被相继报道.与此同时,相应的金属配合物也被广泛应用于不对称金属催化反应中.本文综述了手性环戊二烯配体及其金属配合物的合成研究,介绍了手性环戊二烯金属配合物在不对称C–H键官能团化反应中的应用,同时探讨了该领域当前研究所面临的机遇与挑战.

Rhodium(III)-Catalyzed Atroposelective C-H Indolylization of 1-Aryl Isoquinolines with 3-Indolylphenyliodonium Salts

Rhodium(III)-catalyzed enantioselective C-H indolylization of isoquinolines was developed.The C-H coupling reactions between 1-aryl isoquinoline derivatives and 3-indolylphenyliodonium salts proceeded smoothly in the presence of a modified chiral cyclopentadienyl-Rh(III)complex,affording a series of axially chiral indolylated biaryl products in excellent yields and enantioselectivity(up to 98%yield and 95%ee).

Rhodium-catalyzed enantioselective annulation of N-phenoxyacetamides with 1,3-dienes

Chiral dihydrobenzofuran is a valuable synthetic fragment in both medicinal and organic chemistry.Efficient rhodium(Ⅲ)-catalyzed chemoselective and enantioselective C-H functionalization/[3+2]annulation of N-phenoxyacetamides with 1,3-dienes is reported.In the presence of 5 mol%Cp Rh complex,various chiral dihydrobenzofurans were synthesized in up to 79%yield and 98%ee.This reaction proceeds under mild conditions with excellent functional group compatibility.

基于语义特征提取的砂岩薄片图像颗粒分割方法

砂岩薄片鉴定是矿物学和采矿工程中的一个重要步骤,其基础是将砂岩薄片图像包含的矿物颗粒分割到独立区域.不同于一般图像分割问题,砂岩薄片图像中包含大量矿物颗粒,且相邻颗粒之间边界模糊,通用的图像分割方法难以适用.本文利用多角度砂岩薄片图像,使用卷积神经网络和模糊聚类技术,提出一种3阶段颗粒分割方法.第1阶段,将输入的多角度砂岩图像预分割成超像素集合.第2阶段,根据砂岩矿物特点构建卷积神经网络RockNet,先使用带标签的砂岩矿物颗粒图像库训练RockNet,然后将之用于提取超像素语义特征.第3阶段,提出区域合并方法FCoG,该方法融合多特征用于聚类和合并超像素,并生成最终的矿物颗粒.对采集自多个地区和不同地质年代的砂岩薄片图像数据集进行实验,结果表明本文方法的有效性,其性能明显优于其他分割方法.

Correlation of Vascular Endothelial Growth Factor Production with Photochemical Reaction-induced Retinal Edema

Background： Retinal edema is the major complication of retinal vein occlusion and diabetic retinopathy; it can damage visual function by influencing macular region. This study was to establish a rat retinal edema model and explore the related VEGF expression and observe the responses to anti-VEGF drugs in this model. Methods： A rat retinal edema model was established by inducing photochemical reaction using a 532 nm laser after the intravenous injection of Erythrosin B. Immediately after the laser treatment, models were given intravitreal injections of Ranibizumab or Conbercept to inhibit VEGF expression, and the changes of retinal thickness were measured. Retinal edema was observed using fundus photography （FP）, optical coherence tomography （OCT）, and fluoresce in fundus angiography （FFA） at 0, 1, 2, 4, 7 and 14 days after intervention. The retinal VEGF expression was measured using enzyme-linked immunosorbent assay （ELISA） and western blotting at each time point. The rat retinal edema model was also used to verify the function of anti-VEGF polypeptide ZY1. Results： Both retinal edema and vascular leakage were clearly observed at 1, 2 and 4 days after photochemical induction and the retinal thickness increased notably over the same period. The retinal VEGF expression peaked at day 1 and retina became thickening simultaneously. After the interventions, the VEGF expression of the Ranibizumab and Conbercept groups decreased at each time point compared to the edema group （26.90 ± 3.57 vs. 40.29 ± 6.68, F = 31.269 on day 1 and 22.36 ± 1.12 vs. 29.92 ± 0.93 F = 163.789 on day 2, both P 〈 0.01）; the mean RT （278 ± 4 vs. 288 ± 3, F = 134.190 on day 1 and 274 ± 7 vs. 284 ± 6, F = 64.367 on day 2, both P 〈 0.05） and vascular leakage in these groups also decreased. The same results were observed in the ZY1 group, particularly at day 2 （P 〈 0.05）. Conclusions： This retinal edema model induced by a photochemical reaction is reliable and repeatable. Induced edema increases expression of VEGF. This model can be used to test new drugs.

A Cluster Based Feature Selection Method for Cross-Project Software Defect Prediction

Cross-project defect prediction （CPDP） uses the labeled data from external source software projects to com- pensate the shortage of useful data in the target project, in order to build a meaningful classification model. However, the distribution gap between software features extracted from the source and the target projects may be too large to make the mixed data useful for training. In this paper, we propose a cluster-based novel method FeSCH （Feature Selection Using Clusters of Hybrid-Data） to alleviate the distribution differences by feature selection. FeSCH includes two phases. Tile feature clustering phase clusters features using a density-based clustering method, and the feature selection phase selects features from each cluster using a ranking strategy. For CPDP, we design three different heuristic ranking strategies in the second phase. To investigate the prediction performance of FeSCH, we design experiments based on real-world software projects, and study the effects of design options in FeSCH （such as ranking strategy, feature selection ratio, and classifiers）. The experimental results prove the effectiveness of FeSCH. Firstly, compared with the state-of-the-art baseline methods, FeSCH achieves better performance and its performance is less affected by the classifiers used. Secondly, FeSCH enhances the performance by effectively selecting features across feature categories, and provides guidelines for selecting useful features for defect prediction.