BACKGROUND Emerging studies indicate the critical involvement of microorganisms,such as Epstein-Barr virus(EBV),in the pathogenesis of inflammatory bowel disease(IBD).Immunosuppressive therapies for IBD can reactivate...BACKGROUND Emerging studies indicate the critical involvement of microorganisms,such as Epstein-Barr virus(EBV),in the pathogenesis of inflammatory bowel disease(IBD).Immunosuppressive therapies for IBD can reactivate latent EBV,complicating the clinical course of IBD.Moreover,the clinical significance of EBV expression in B lymphocytes derived from IBD patients’intestinal tissues has not been explored in detail.AIM To explore the clinical significance of latent EBV infection in IBD patients.METHODS Latent EBV infection was determined by double staining for EBV encoded RNA and CD20 in colon specimens of 43 IBD patients who underwent bowel resection.Based on the staining results,the patients were divided into two groups,according to their latent EBV infection states-negative(n=33)and positive(n=10).Illness severity of IBD were assigned according to Crohn’s disease activity index(ulcerative colitis)and Mayo staging system(Crohn’s disease).The clinicpathological data were analyzed between the two different latent EBV groups and also between the mild-to-moderate and severe disease groups.RESULTS Systolic pressure(P=0.005),variety of disease(P=0.005),the severity of illness(P=0.002),and pre-op corticosteroids(P=0.025)were significantly different between the EBV-negative and EBV-positive groups.Systolic pressure(P=0.001),variety of disease(P=0.000),pre-op corticosteroids(P=0.011)and EBV infection(P=0.003)were significantly different between the mild-to-moderate and severe disease groups.CONCLUSION IBD patients with latent EBV infection may manifest more severe illnesses.It is suggested that the role of EBV in IBD development should be further investigated,latent EBV infection in patients with serious IBD should be closely monitored,and therapeutic course should be optimized.展开更多
The pressure and horizontal particle velocity combined descriptions in the very low frequency acoustic field of shallow wa- ter integrated with the concept of effective depth of Pekeris wave- guide is proposed, especi...The pressure and horizontal particle velocity combined descriptions in the very low frequency acoustic field of shallow wa- ter integrated with the concept of effective depth of Pekeris wave- guide is proposed, especially the active component of the pressure and horizontal particle velocity cross-spectrum, also called ho- rizontal complex cross acoustic intensity, when only two normal modes are trapped in the waveguide. Both the approximate theo- retic analysis and the numerical results show that the sign of the horizontal complex cross acoustic intensity active component is independent of the range when vertically deployed receiving dual sensors are placed in appropriate depths, the sum of which is equal to the waveguide effective depth, so it can be used to tell whether the sound source is near the surface or underwater; while the range rate is expected to be measured by utilizing the sign distribution characteristic of the reactive component. The further robustness analysis of the depth classification algorithm shows that the existence of shear waves in semi infinite basement and the change of acoustic velocity profiles have few effects on the application of this method, and the seabed attenuation will limit the detection range, but the algorithm still has a good robustness in the valid detection range.展开更多
BACKGROUND Management of non-neurogenic,non-obstructive dysuria represents one of the most challenging dilemmas in urological practice.The main clinical symptom is the increase in residual urine.Voiding dysfunction is...BACKGROUND Management of non-neurogenic,non-obstructive dysuria represents one of the most challenging dilemmas in urological practice.The main clinical symptom is the increase in residual urine.Voiding dysfunction is the main cause of dysuria or urinary retention,mainly due to the decrease in bladder contraction(the decrease in contraction amplitude or duration)or the increase in outflow tract resistance.Sacral neuromodulation(SNM)has been used for>10 years to treat many kinds of lower urinary tract dysfunction.It has become increasingly popular in China in recent years.Consequently,studies focusing on nonneurogenic,non-obstructive dysuria patients treated by SNM are highly desirable.AIM To assess the outcome of two-stage SNM in non-neurogenic,non-obstructive dysuria.METHODS Clinical data of 54 patients(26 men,28 women)with non-neurogenic,nonobstructive dysuria treated by SNM from January 2012 to December 2016 in ten medical centers in China were retrospectively analyzed.All patients received two or more conservative treatments.The voiding diary,urgency score,and quality of life score before operation,after implantation of tined lead in stage I(test period),and during short-term follow-up(latest follow-up)after implantation of the implanted pulse generator in stage II were compared to observe symptom improvements.RESULTS Among the 54 study patients,eight refused to implant an implanted pulse generator because of the unsatisfactory effect,and 46 chose to embed the implanted pulse generator at the end of stage I.The conversion rate of stage I to stage II was 85.2%.The average follow-up time was 18.6 mo.There were significant differences between baseline(before stage I)and the test period(after stage I)in residual urine,voiding frequency,average voiding amount,maximum voiding amount,nocturia,urgency score,and quality of life score.The residual urine and urgency score between the test period and the latest follow-up time(after stage II)were also significantly different.No significant differences were observed for other parameters.No wound infection,electrode breakage,or other irreversible adverse events occurred.CONCLUSION SNM is effective for patients with non-neurogenic,non-obstructive dysuria showing a poor response to traditional treatment.The duration of continuous stimulation may be positively correlated with the improvement of residual urine.展开更多
Acyl-CoA synthetase long chain family member 5(ACSL5),is a member of the acyl-CoA synthetases(ACSs)family that activates long chain fatty acids by catalyzing the synthesis of fatty acyl-CoAs.The dysregulation of ACSL5...Acyl-CoA synthetase long chain family member 5(ACSL5),is a member of the acyl-CoA synthetases(ACSs)family that activates long chain fatty acids by catalyzing the synthesis of fatty acyl-CoAs.The dysregulation of ACSL5 has been reported in some cancers,such as glioma and colon cancers.However,little is known about the role of ACSL5 in acute myeloid leukemia(AML).We found that the expression of ACSL5 was higher in bone marrow cells from AML patients compared with that from healthy donors.ACSL5 level could serve as an independent prognostic predictor of the overall survival of AML patients.In AML cells,the ACSL5 knockdown inhibited cell growth both in vitro and in vivo.Mechanistically,the knockdown of ACSL5 suppressed the activation of the Wnt/β-catenin pathway by suppressing the palmitoylation modification of Wnt3a.Additionally,triacsin c,a pan-ACS family inhibitor,inhibited cell growth and robustly induced cell apoptosis when combined with ABT-199,the FDA approved BCL-2 inhibitor for AML therapy.Our results indicate that ACSL5 is a potential prognosis marker for AML and a promising pharmacological target for the treatment of molecularly stratified AML.展开更多
Idiopathic hypogonadotropic hypogonadism(IHH)is a rare genetic disease with clinical and genetic heterogeneity.This study aimed to investigate a novel causal gene of IHH and a homozygous mutation(p.Ala515Val)in SEMA4D...Idiopathic hypogonadotropic hypogonadism(IHH)is a rare genetic disease with clinical and genetic heterogeneity.This study aimed to investigate a novel causal gene of IHH and a homozygous mutation(p.Ala515Val)in SEMA4D,and sought to determine the mechanism of SEMA4D promoting GnRH neurons migration.展开更多
Due to various technical issues,existing numerical weather prediction(NWP)models often perform poorly at forecasting rainfall in the first several hours.To correct the bias of an NWP model and improve the accuracy of ...Due to various technical issues,existing numerical weather prediction(NWP)models often perform poorly at forecasting rainfall in the first several hours.To correct the bias of an NWP model and improve the accuracy of short-range precipitation forecasting,we propose a deep learning-based approach called UNet Mask,which combines NWP forecasts with the output of a convolutional neural network called UNet.The UNet Mask involves training the UNet on historical data from the NWP model and gridded rainfall observations for 6-hour precipitation forecasting.The overlap of the UNet output and the NWP forecasts at the same rainfall threshold yields a mask.The UNet Mask blends the UNet output and the NWP forecasts by taking the maximum between them and passing through the mask,which provides the corrected 6-hour rainfall forecasts.We evaluated UNet Mask on a test set and in real-time verification.The results showed that UNet Mask outperforms the NWP model in 6-hour precipitation prediction by reducing the FAR and improving CSI scores.Sensitivity tests also showed that different small rainfall thresholds applied to the UNet and the NWP model have different effects on UNet Mask's forecast performance.This study shows that UNet Mask is a promising approach for improving rainfall forecasting of NWP models.展开更多
目的评价年轻男男性行为者(young men who have sex with men,YMSM)艾滋病行为干预模式效果。方法采用滚雪球抽样法,在四川省绵阳市招募15~24岁的艾滋病病毒(HIV)阴性YMSM,实施"心灵冲击与持续强化相结合的行为干预",建立12...目的评价年轻男男性行为者(young men who have sex with men,YMSM)艾滋病行为干预模式效果。方法采用滚雪球抽样法,在四川省绵阳市招募15~24岁的艾滋病病毒(HIV)阴性YMSM,实施"心灵冲击与持续强化相结合的行为干预",建立12个月随访队列。通过基线及随访调查了解HIV、梅毒新发感染和采用广义估计方程分析知-信-行变化,评价干预效果。结果队列入组328人,随访调查198人(60.37%),HIV、梅毒新发感染率分别为1.31/100人年、1.41/100人年。随访较基线调查:艾滋病知识知晓率、风险认识提高(P均<0.05),近6个月性行为每次准备安全套从63.64%上升到71.79%、没有安全套会去买/非插入性行为从59.18%上升到84.44%、有1个固定性伴从55.43%上升到74.77%、≥2个偶然性伴从63.16%下降到45.00%、近1次有保护肛交从83.33%上升到91.55%,近6个月有保护肛交从61.90%上升到80.99%(P均<0.05)。结论YMSM随访队列HIV新发感染率居较低水平,艾滋病知识、风险认知和行为明显改变,干预模式切实有效。展开更多
基金Supported by Clinical and Translational Medicine Research Foundation of Chinese Academy of Medical Sciences,No. 2020-I2M-C&T-B-038Capital Health Research and Development of Special Project,No. 2022-1-2181Group Medical Aid Project of the Tibet Autonomous Region Natural Science Foundation,No. XZ2020ZR-ZY28[Z]
文摘BACKGROUND Emerging studies indicate the critical involvement of microorganisms,such as Epstein-Barr virus(EBV),in the pathogenesis of inflammatory bowel disease(IBD).Immunosuppressive therapies for IBD can reactivate latent EBV,complicating the clinical course of IBD.Moreover,the clinical significance of EBV expression in B lymphocytes derived from IBD patients’intestinal tissues has not been explored in detail.AIM To explore the clinical significance of latent EBV infection in IBD patients.METHODS Latent EBV infection was determined by double staining for EBV encoded RNA and CD20 in colon specimens of 43 IBD patients who underwent bowel resection.Based on the staining results,the patients were divided into two groups,according to their latent EBV infection states-negative(n=33)and positive(n=10).Illness severity of IBD were assigned according to Crohn’s disease activity index(ulcerative colitis)and Mayo staging system(Crohn’s disease).The clinicpathological data were analyzed between the two different latent EBV groups and also between the mild-to-moderate and severe disease groups.RESULTS Systolic pressure(P=0.005),variety of disease(P=0.005),the severity of illness(P=0.002),and pre-op corticosteroids(P=0.025)were significantly different between the EBV-negative and EBV-positive groups.Systolic pressure(P=0.001),variety of disease(P=0.000),pre-op corticosteroids(P=0.011)and EBV infection(P=0.003)were significantly different between the mild-to-moderate and severe disease groups.CONCLUSION IBD patients with latent EBV infection may manifest more severe illnesses.It is suggested that the role of EBV in IBD development should be further investigated,latent EBV infection in patients with serious IBD should be closely monitored,and therapeutic course should be optimized.
基金supported by the National Natural Science Foundation of China(1140440611374072)
文摘The pressure and horizontal particle velocity combined descriptions in the very low frequency acoustic field of shallow wa- ter integrated with the concept of effective depth of Pekeris wave- guide is proposed, especially the active component of the pressure and horizontal particle velocity cross-spectrum, also called ho- rizontal complex cross acoustic intensity, when only two normal modes are trapped in the waveguide. Both the approximate theo- retic analysis and the numerical results show that the sign of the horizontal complex cross acoustic intensity active component is independent of the range when vertically deployed receiving dual sensors are placed in appropriate depths, the sum of which is equal to the waveguide effective depth, so it can be used to tell whether the sound source is near the surface or underwater; while the range rate is expected to be measured by utilizing the sign distribution characteristic of the reactive component. The further robustness analysis of the depth classification algorithm shows that the existence of shear waves in semi infinite basement and the change of acoustic velocity profiles have few effects on the application of this method, and the seabed attenuation will limit the detection range, but the algorithm still has a good robustness in the valid detection range.
基金Supported by National Key Research and Development Program of China,No.2018YFC2002202.
文摘BACKGROUND Management of non-neurogenic,non-obstructive dysuria represents one of the most challenging dilemmas in urological practice.The main clinical symptom is the increase in residual urine.Voiding dysfunction is the main cause of dysuria or urinary retention,mainly due to the decrease in bladder contraction(the decrease in contraction amplitude or duration)or the increase in outflow tract resistance.Sacral neuromodulation(SNM)has been used for>10 years to treat many kinds of lower urinary tract dysfunction.It has become increasingly popular in China in recent years.Consequently,studies focusing on nonneurogenic,non-obstructive dysuria patients treated by SNM are highly desirable.AIM To assess the outcome of two-stage SNM in non-neurogenic,non-obstructive dysuria.METHODS Clinical data of 54 patients(26 men,28 women)with non-neurogenic,nonobstructive dysuria treated by SNM from January 2012 to December 2016 in ten medical centers in China were retrospectively analyzed.All patients received two or more conservative treatments.The voiding diary,urgency score,and quality of life score before operation,after implantation of tined lead in stage I(test period),and during short-term follow-up(latest follow-up)after implantation of the implanted pulse generator in stage II were compared to observe symptom improvements.RESULTS Among the 54 study patients,eight refused to implant an implanted pulse generator because of the unsatisfactory effect,and 46 chose to embed the implanted pulse generator at the end of stage I.The conversion rate of stage I to stage II was 85.2%.The average follow-up time was 18.6 mo.There were significant differences between baseline(before stage I)and the test period(after stage I)in residual urine,voiding frequency,average voiding amount,maximum voiding amount,nocturia,urgency score,and quality of life score.The residual urine and urgency score between the test period and the latest follow-up time(after stage II)were also significantly different.No significant differences were observed for other parameters.No wound infection,electrode breakage,or other irreversible adverse events occurred.CONCLUSION SNM is effective for patients with non-neurogenic,non-obstructive dysuria showing a poor response to traditional treatment.The duration of continuous stimulation may be positively correlated with the improvement of residual urine.
基金supported by the key international cooperation projects of the National Natural Science Foundation of China(No.81820108004)the major projects of the Zhejiang Provincial Department of Science and Technology(No.2021C03123)the Pediatric Leukemia Diagnosis and Therapeutic Technology Research Center of Zhejiang Province(No.JBZX-201904).
文摘Acyl-CoA synthetase long chain family member 5(ACSL5),is a member of the acyl-CoA synthetases(ACSs)family that activates long chain fatty acids by catalyzing the synthesis of fatty acyl-CoAs.The dysregulation of ACSL5 has been reported in some cancers,such as glioma and colon cancers.However,little is known about the role of ACSL5 in acute myeloid leukemia(AML).We found that the expression of ACSL5 was higher in bone marrow cells from AML patients compared with that from healthy donors.ACSL5 level could serve as an independent prognostic predictor of the overall survival of AML patients.In AML cells,the ACSL5 knockdown inhibited cell growth both in vitro and in vivo.Mechanistically,the knockdown of ACSL5 suppressed the activation of the Wnt/β-catenin pathway by suppressing the palmitoylation modification of Wnt3a.Additionally,triacsin c,a pan-ACS family inhibitor,inhibited cell growth and robustly induced cell apoptosis when combined with ABT-199,the FDA approved BCL-2 inhibitor for AML therapy.Our results indicate that ACSL5 is a potential prognosis marker for AML and a promising pharmacological target for the treatment of molecularly stratified AML.
基金The project was supported by the National Natural Science Foundation of China(No.81671443 to J.L.,No.81601270 to H.X.)Tongji Hospital Clinical Research Flash Ship Program(China)(No.2019CR109).
文摘Idiopathic hypogonadotropic hypogonadism(IHH)is a rare genetic disease with clinical and genetic heterogeneity.This study aimed to investigate a novel causal gene of IHH and a homozygous mutation(p.Ala515Val)in SEMA4D,and sought to determine the mechanism of SEMA4D promoting GnRH neurons migration.
基金supported by the Strategic Priority Research Programme of the Chinese Academy of Sciences(XDA20060302)the Tianshan Talent Cultivation(2022TSYCLJ0001)+2 种基金the Key Projects of Natural Science Foundation of Xinjiang Uygur Autonomous Region(2022D01D01)the National Natural Science Foundation of China(U1803243)the High-End Foreign Experts Project(G2022045012L)。
基金jointly supported by the National Natural Science Foundation of China(Grant No.U1811464)the Hydraulic Innovation Project of Science and Technology of Guangdong Province of China(Grant No.2022-01)the Guangzhou Basic and Applied Basic Research Foundation(Grant No.202201011472)。
文摘Due to various technical issues,existing numerical weather prediction(NWP)models often perform poorly at forecasting rainfall in the first several hours.To correct the bias of an NWP model and improve the accuracy of short-range precipitation forecasting,we propose a deep learning-based approach called UNet Mask,which combines NWP forecasts with the output of a convolutional neural network called UNet.The UNet Mask involves training the UNet on historical data from the NWP model and gridded rainfall observations for 6-hour precipitation forecasting.The overlap of the UNet output and the NWP forecasts at the same rainfall threshold yields a mask.The UNet Mask blends the UNet output and the NWP forecasts by taking the maximum between them and passing through the mask,which provides the corrected 6-hour rainfall forecasts.We evaluated UNet Mask on a test set and in real-time verification.The results showed that UNet Mask outperforms the NWP model in 6-hour precipitation prediction by reducing the FAR and improving CSI scores.Sensitivity tests also showed that different small rainfall thresholds applied to the UNet and the NWP model have different effects on UNet Mask's forecast performance.This study shows that UNet Mask is a promising approach for improving rainfall forecasting of NWP models.
文摘目的评价年轻男男性行为者(young men who have sex with men,YMSM)艾滋病行为干预模式效果。方法采用滚雪球抽样法,在四川省绵阳市招募15~24岁的艾滋病病毒(HIV)阴性YMSM,实施"心灵冲击与持续强化相结合的行为干预",建立12个月随访队列。通过基线及随访调查了解HIV、梅毒新发感染和采用广义估计方程分析知-信-行变化,评价干预效果。结果队列入组328人,随访调查198人(60.37%),HIV、梅毒新发感染率分别为1.31/100人年、1.41/100人年。随访较基线调查:艾滋病知识知晓率、风险认识提高(P均<0.05),近6个月性行为每次准备安全套从63.64%上升到71.79%、没有安全套会去买/非插入性行为从59.18%上升到84.44%、有1个固定性伴从55.43%上升到74.77%、≥2个偶然性伴从63.16%下降到45.00%、近1次有保护肛交从83.33%上升到91.55%,近6个月有保护肛交从61.90%上升到80.99%(P均<0.05)。结论YMSM随访队列HIV新发感染率居较低水平,艾滋病知识、风险认知和行为明显改变,干预模式切实有效。
