KCNQ1 has been identified as a susceptibility gene of type 2 diabetes mellitus （T2DM） in Asian populations through genome-wide association studies. However, studies on the association between gene polymorphism of KC...KCNQ1 has been identified as a susceptibility gene of type 2 diabetes mellitus （T2DM） in Asian populations through genome-wide association studies. However, studies on the association between gene polymorphism of KCNQ1 and T2DM complications remain unclear. To further ana- lyze the association between different alleles at the single nncleotide polymorphism （SNP） rs2237892 within KCNQ1 and TD2M and its complications, we conducted a case-control study in a Chinese Han population. The C allele of rs2237892 variant contributed to susceptibility to T2DM （odds ratio [OR], 1.45; 95% confidence interval [CI], 1.20 1.75）. Genotypes CT （OR, 1.97; 95% CI, 1.24-3.15） and CC （OR, 2.49; 95% CI, 1.57-3.95） were associated with an increased risk of T2DM. Multivariate regression analysis was performed with adjustment of age, gender, and body mass index. We found that systolic blood pressure （P = 0.015）, prevalence of hypertension （P = 0.037）, and risk of maerovascnlar disease （OR, 2.10; CI, 1.00-4.45） were significantly higher in subjects with the CC genotype than in the combined population with genotype either CT or TT. Therefore, our data support that KCNQ1 might contribute to the higher incidence of patients with T2DM carrying the risk allele C population. is associated with an increased risk for T2DM and hypertension and macrovascular complications in though it needs further to be confirmed in a larger展开更多
Accumulating evidence suggests that a reduction in the number of Foxp3^(+) regulatory T cells(Tregs)contributes to the pathogenesis of acute graft-versus-host disease(aGVHD),which is a major adverse complication that ...Accumulating evidence suggests that a reduction in the number of Foxp3^(+) regulatory T cells(Tregs)contributes to the pathogenesis of acute graft-versus-host disease(aGVHD),which is a major adverse complication that can occur after allogeneic hematopoietic stem cell transplantation(allo-HSCT).However,the precise features and mechanism underlying the defects in Tregs remain largely unknown.In this study,we demonstrated that Tregs were more dramatically decreased in bone marrow compared with those in peripheral blood from aGVHD patients and that bone marrow Treg defects were negatively associated with hematopoietic reconstitution.Tregs from aGVHD patients exhibited multiple defects,including the instability of Foxp3 expression,especially in response to IL-12,impaired suppressor function,decreased migratory capacity,and increased apoptosis.Transcriptional profiling revealed the downregulation of Lkb1,a previously identified critical regulator of murine Treg identity and metabolism,and murine Lkb1-regulated genes in Tregs from aGVHD patients.Foxp3 expression in human Tregs could be decreased and increased by the knockdown and overexpression of the Lkb1 gene,respectively.Furthermore,a loss-of-function assay in an aGVHD murine model confirmed that Lkb1 deficiency could impair Tregs and aggravate disease severity.These findings reveal that Lkb1 downregulation contributes to multiple defects in Tregs in human aGVHD and highlight the Lkb1-related pathways that could serve as therapeutic targets that may potentially be manipulated to mitigate aGVHD.展开更多
Converting CO_(2)into carbonaceous fuels via photocatalysis represents an appealing strategy to simultaneously alleviate the energy crisis and associated environmental problems,yet designing with high photoreduction a...Converting CO_(2)into carbonaceous fuels via photocatalysis represents an appealing strategy to simultaneously alleviate the energy crisis and associated environmental problems,yet designing with high photoreduction activity catalysts remains a compelling challenge.Here,combining the merits of highly porous structure and maximum atomic efficiency,we rationally constructed covalent triazine-based frameworks(CTFs)anchoring copper single atoms(Cu-SA/CTF)photocatalysts for efficient CO_(2)conversion.The Cu single atoms were visualized by high-angle annular dark-field scanning transmission electron microscopy(HAADF-STEM)images and coordination structure of Cu-N-C2 sites was revealed by extended X-ray absorption fine structure(EXAFS)analyses.The as-prepared Cu-SA/CTF photocatalysts exhibited superior photocatalytic CO_(2)conversion to CH4 performance associated with a high selectivity of 98.31%.Significantly,the introduction of Cu single atoms endowed the CuSA/CTF catalysts with increased CO_(2)adsorption capacity,strengthened visible light responsive ability,and improved the photogenerated carriers separation efficiency,thus enhancing the photocatalytic activity.This work provides useful guidelines for designing robust visible light responsive photoreduction CO_(2)catalysts on the atomic scale.展开更多
Single-atom catalysts(SACs)have emerged as an advanced frontier in heterogeneous catalysis due to their potential to maximize the atomic efficiency.Herein,covalent triazine-based frameworks(CTFs)confining cobalt singl...Single-atom catalysts(SACs)have emerged as an advanced frontier in heterogeneous catalysis due to their potential to maximize the atomic efficiency.Herein,covalent triazine-based frameworks(CTFs)confining cobalt single atoms(Co-SA/CTF)photocatalysts have been synthesized and used for efficient CO_(2) reduction and hydrogen production under visible light irradiation.The resulted Co-SA/CTF demonstrate excellent photocatalytic activity,with the CO and H2 evolution rates reaching 1665.74μmol g^(−1) h^(−1) and 1293.18μmol g^(−1) h^(−1),respectively,far surpassing those of Co nanoparticles anchored CTF and pure CTF.A variety of instrumental analyses collectively indicated that Co single atoms sites served as the reaction center for activating the adsorbed CO_(2) molecules,which significantly improved the CO_(2) reduction performance.Additionally,the introduction of Co single atoms could accelerate the separation/transfer of photogenerated charge carriers,thus boosting the photocatalytic performance.This study envisions a novel strategy for designing efficient photocatalysts for energy conversion and showcases the application of CTFs as attractive support for confining metal single atoms.展开更多
Background:The estimation of postmortem interval(PMI)is one of the most important topics in forensic medicine research.We speculate that with an increased PMI,the computed tomography(CT)values of different tissues may...Background:The estimation of postmortem interval(PMI)is one of the most important topics in forensic medicine research.We speculate that with an increased PMI,the computed tomography(CT)values of different tissues may show regular changes.Purpose:To use postmortem computed tomography(PMCT)to measure the myocardial CT value(unit:Hounsfield Unit,HU)of the heart to explore its pattern in postmortem change,and to discuss whether it can serve as a new parameter for PMI estimation.Methods:A total of 10 healthy adult New Zealand rabbits were selected and then put into a 20℃ incubator after being sacrificed.Within 0-156 h after death,CT scans were performed every 12 h to detect changes in the myocardial CT value of the heart over time.Regression analysis was used to determine the relationship between the myocardial CT value of the heart and PMI.At the same time,HE and Masson were used to stain the cardiac tissue sections detected by PMCT at Oh,48h and 156h,respectively.Results:During 0-156 h,the overall myocardial CT value showed a trend of first rising and then decreasing with the increase of PMI.The fitting regression equation was y=-2873.193+143.866x-1.728x2(x:myocardial CT value,unit:Hu;y:PMI,unit:h,R2=0.466,P<0.05).During 48-156 h,the overall myocardial CT value decreased gradually with the increase of PMI.The fitting regression equation was y=-93.038+18.700x-0.321x2(x:myocardial CT value,unit:Hu;y:PMI,unit:h,R2=0.963,P<0.001).The results of the morphological changes of the myocardial tissue structure after death showed that the myocardial cell structure was relatively complete at 0-48 hours after death;and the myocardial cell structure disappeared at 156 hours after death.Conclusions:Our results revealed evident postmortem changes in the myocardial CT value of the heart.Accordingly,measuring the myocardial CT value through PMCT shows promise for being used as a parameter for PMI estimation in forensic medicine and is worthy of further studies.The morphological changes of the myocardial tissue structure after death provide morphological basis for postmortem changes of tissue density,and further prove the reasons for the changes of CT value.展开更多
基金supported by the National Natural Science Foundation of China(Grant No. 81271918)Zhejiang Provincial Natural Science Foundation of China (Grant No. Y2090753)+2 种基金Key Science and Technology Innovation Team of Zhejiang Province (Grant No. 2010R50048)Zhejiang Provincial Program for the Cultivation of High-level Innovative Health Talentsthe Medical Scientific Projects from the Health Bureau of Zhejiang Province (Grant No. 2011ZDA016)
文摘KCNQ1 has been identified as a susceptibility gene of type 2 diabetes mellitus （T2DM） in Asian populations through genome-wide association studies. However, studies on the association between gene polymorphism of KCNQ1 and T2DM complications remain unclear. To further ana- lyze the association between different alleles at the single nncleotide polymorphism （SNP） rs2237892 within KCNQ1 and TD2M and its complications, we conducted a case-control study in a Chinese Han population. The C allele of rs2237892 variant contributed to susceptibility to T2DM （odds ratio [OR], 1.45; 95% confidence interval [CI], 1.20 1.75）. Genotypes CT （OR, 1.97; 95% CI, 1.24-3.15） and CC （OR, 2.49; 95% CI, 1.57-3.95） were associated with an increased risk of T2DM. Multivariate regression analysis was performed with adjustment of age, gender, and body mass index. We found that systolic blood pressure （P = 0.015）, prevalence of hypertension （P = 0.037）, and risk of maerovascnlar disease （OR, 2.10; CI, 1.00-4.45） were significantly higher in subjects with the CC genotype than in the combined population with genotype either CT or TT. Therefore, our data support that KCNQ1 might contribute to the higher incidence of patients with T2DM carrying the risk allele C population. is associated with an increased risk for T2DM and hypertension and macrovascular complications in though it needs further to be confirmed in a larger
基金by grants from the National Basic Research Program of China(2015CB964402)the National Natural Science Foundation of China(81670171,81601369)+2 种基金the CAMS Innovation Fund for Medical Sciences(CIFMS,2016-I2M-1-003 and 2018-I2M-HL-013)the Tianjin Science Funds for Distinguished Young Scholars(17JCJQJC45800)the Nonprofit Central Research Institute Fund of the Chinese Academy of Medical Sciences(2018PT32034 and 2019-RC-HL-013).
文摘Accumulating evidence suggests that a reduction in the number of Foxp3^(+) regulatory T cells(Tregs)contributes to the pathogenesis of acute graft-versus-host disease(aGVHD),which is a major adverse complication that can occur after allogeneic hematopoietic stem cell transplantation(allo-HSCT).However,the precise features and mechanism underlying the defects in Tregs remain largely unknown.In this study,we demonstrated that Tregs were more dramatically decreased in bone marrow compared with those in peripheral blood from aGVHD patients and that bone marrow Treg defects were negatively associated with hematopoietic reconstitution.Tregs from aGVHD patients exhibited multiple defects,including the instability of Foxp3 expression,especially in response to IL-12,impaired suppressor function,decreased migratory capacity,and increased apoptosis.Transcriptional profiling revealed the downregulation of Lkb1,a previously identified critical regulator of murine Treg identity and metabolism,and murine Lkb1-regulated genes in Tregs from aGVHD patients.Foxp3 expression in human Tregs could be decreased and increased by the knockdown and overexpression of the Lkb1 gene,respectively.Furthermore,a loss-of-function assay in an aGVHD murine model confirmed that Lkb1 deficiency could impair Tregs and aggravate disease severity.These findings reveal that Lkb1 downregulation contributes to multiple defects in Tregs in human aGVHD and highlight the Lkb1-related pathways that could serve as therapeutic targets that may potentially be manipulated to mitigate aGVHD.
基金the National Natural Science Foundation of China(Nos.51672047,21707173,and 21701168)Dalian high level talent innovation project(No.2019RQ063)+2 种基金the National Natural Science Foundation of Fujian Province(Nos.2019J01648 and 2019J01226)Open project Foundation of State Key Laboratory of Structural Chemistry,Fujian Institute of Research on the Structure of Matter,Chinese Academy of Sciences(No.20200021)the Youth Talent Support Program of Fujian Province(No.00387077).
文摘Converting CO_(2)into carbonaceous fuels via photocatalysis represents an appealing strategy to simultaneously alleviate the energy crisis and associated environmental problems,yet designing with high photoreduction activity catalysts remains a compelling challenge.Here,combining the merits of highly porous structure and maximum atomic efficiency,we rationally constructed covalent triazine-based frameworks(CTFs)anchoring copper single atoms(Cu-SA/CTF)photocatalysts for efficient CO_(2)conversion.The Cu single atoms were visualized by high-angle annular dark-field scanning transmission electron microscopy(HAADF-STEM)images and coordination structure of Cu-N-C2 sites was revealed by extended X-ray absorption fine structure(EXAFS)analyses.The as-prepared Cu-SA/CTF photocatalysts exhibited superior photocatalytic CO_(2)conversion to CH4 performance associated with a high selectivity of 98.31%.Significantly,the introduction of Cu single atoms endowed the CuSA/CTF catalysts with increased CO_(2)adsorption capacity,strengthened visible light responsive ability,and improved the photogenerated carriers separation efficiency,thus enhancing the photocatalytic activity.This work provides useful guidelines for designing robust visible light responsive photoreduction CO_(2)catalysts on the atomic scale.
基金financially supported by the National Natural Science Foundation of China(Nos.51672047,21707173)the Youth Talent Support Program of Fujian Province(00387077)the National Natural Science Foundation of Fujian Province(Nos.2019J01648,2019J01226)。
文摘Single-atom catalysts(SACs)have emerged as an advanced frontier in heterogeneous catalysis due to their potential to maximize the atomic efficiency.Herein,covalent triazine-based frameworks(CTFs)confining cobalt single atoms(Co-SA/CTF)photocatalysts have been synthesized and used for efficient CO_(2) reduction and hydrogen production under visible light irradiation.The resulted Co-SA/CTF demonstrate excellent photocatalytic activity,with the CO and H2 evolution rates reaching 1665.74μmol g^(−1) h^(−1) and 1293.18μmol g^(−1) h^(−1),respectively,far surpassing those of Co nanoparticles anchored CTF and pure CTF.A variety of instrumental analyses collectively indicated that Co single atoms sites served as the reaction center for activating the adsorbed CO_(2) molecules,which significantly improved the CO_(2) reduction performance.Additionally,the introduction of Co single atoms could accelerate the separation/transfer of photogenerated charge carriers,thus boosting the photocatalytic performance.This study envisions a novel strategy for designing efficient photocatalysts for energy conversion and showcases the application of CTFs as attractive support for confining metal single atoms.
基金supported by the General Program of National Natural Science Foundation of China(grant number 81971796),Bering Natural Science Foundation(grant number 7192121)Chinese Academy of Engineering Advisory Project(grant number 2019-XZ-31)Production-Study-Research Project for graduate students of China University of Political Science and Law(grant number CXY2012).
文摘Background:The estimation of postmortem interval(PMI)is one of the most important topics in forensic medicine research.We speculate that with an increased PMI,the computed tomography(CT)values of different tissues may show regular changes.Purpose:To use postmortem computed tomography(PMCT)to measure the myocardial CT value(unit:Hounsfield Unit,HU)of the heart to explore its pattern in postmortem change,and to discuss whether it can serve as a new parameter for PMI estimation.Methods:A total of 10 healthy adult New Zealand rabbits were selected and then put into a 20℃ incubator after being sacrificed.Within 0-156 h after death,CT scans were performed every 12 h to detect changes in the myocardial CT value of the heart over time.Regression analysis was used to determine the relationship between the myocardial CT value of the heart and PMI.At the same time,HE and Masson were used to stain the cardiac tissue sections detected by PMCT at Oh,48h and 156h,respectively.Results:During 0-156 h,the overall myocardial CT value showed a trend of first rising and then decreasing with the increase of PMI.The fitting regression equation was y=-2873.193+143.866x-1.728x2(x:myocardial CT value,unit:Hu;y:PMI,unit:h,R2=0.466,P<0.05).During 48-156 h,the overall myocardial CT value decreased gradually with the increase of PMI.The fitting regression equation was y=-93.038+18.700x-0.321x2(x:myocardial CT value,unit:Hu;y:PMI,unit:h,R2=0.963,P<0.001).The results of the morphological changes of the myocardial tissue structure after death showed that the myocardial cell structure was relatively complete at 0-48 hours after death;and the myocardial cell structure disappeared at 156 hours after death.Conclusions:Our results revealed evident postmortem changes in the myocardial CT value of the heart.Accordingly,measuring the myocardial CT value through PMCT shows promise for being used as a parameter for PMI estimation in forensic medicine and is worthy of further studies.The morphological changes of the myocardial tissue structure after death provide morphological basis for postmortem changes of tissue density,and further prove the reasons for the changes of CT value.
