AIM:To access the agreement of intraocular pressure(IOP)values obtained from biomechanically corrected tonometer[Corvis ST(CST)],non-contact tonometer(NCT),and Goldmann applanation tonometer(GAT)in children with NCT m...AIM:To access the agreement of intraocular pressure(IOP)values obtained from biomechanically corrected tonometer[Corvis ST(CST)],non-contact tonometer(NCT),and Goldmann applanation tonometer(GAT)in children with NCT measured-IOP(NCT-IOP)values of 22 mm Hg or more,and related factors.METHODS:A total of 51 eyes with NCT-IOP≥22 mm Hg in children aged 7 to 14y were examined and IOP was measured by CST,NCT,and GAT.Based on GAT measured IOP(GAT-IOP),ocular hypertension(OHT)group(≥22 mm Hg,24 eyes)and the non-OHT group(<22 mm Hg,27 eyes)were defined.We compared the agreement of the three measurements,i.e.,CST measured IOP(CST-IOP),GAT-IOP,and NCT-IOP,and further analyzed the correlation between the differences in tonometry readings,central corneal thickness(CCT),axial length(AL),optic disc rim volume,and age.RESULTS:Compared with the OHT group,thicker CCT,larger rim volume,and higher differences between NCTIOP and GAT-IOP,were found in the non-OHT group.The differences between CST-IOP and GAT-IOP were lower than the differences between NCT-IOP and GAT-IOP in both groups.The mean differences in CST-IOP and GAT-IOP were 1.26 mm Hg(95%limit of agreement ranged from 0.1 to 2.41 mm Hg,OHT group)and 1.20 mm Hg(95%limit of agreement ranged from-0.5 to 3.00 mm Hg,non-OHT group),and the mean differences in NCT and GAT were 3.90 mm Hg(95%limit of agreement ranged from-0.19 to 9.70 mm Hg,OHT group)and 6.00 mm Hg(95%limit of agreement ranged from 1.50 to 10.50 mm Hg,non-OHT group).The differences between CST-IOP and GAT-IOP were not related to CCT,age,and AL in both groups;while the differences between NCT-IOP and GAT-IOP were related to CCT in the OHT group(r=0.93,P<0.001)and to CCT and AL in the non-OHT group(r=0.66,P<0.001,r=-0.81,P<0.001).CONCLUSION:The accuracy of NCT in the diagnosis of pediatric OHT is low.The agreement of CST-IOP and GATIOP was significantly higher in children with and without OHT than in those with NCT-IOP and GAT-IOP.Therefore,CST can be used as a good alternative for IOP measurement in children.The impacts of CCT and AL on NCT measurement need to be fully considered when managing childhood IOP.展开更多
AIM: To examine fibroblast activation protein (FAP) expression in pancreatic ductal adenocarcinoma (PDAC) and to analyze its relationship with the clinicopathology of PDAC. METHODS: FAP expression was examined in 134 ...AIM: To examine fibroblast activation protein (FAP) expression in pancreatic ductal adenocarcinoma (PDAC) and to analyze its relationship with the clinicopathology of PDAC. METHODS: FAP expression was examined in 134 PDAC specimens by immunohistochemistry, and in four pancreatic cancer cell lines (SW1990, Miapaca-2, AsPC-1 and BxPC-3) by Western blotting assay. We also analyzed the association between FAP expression in PDAC cells and the clinicopathology of PDAC patients. RESULTS: The results showed that the FAP was expressed in both stromal fibroblast cells (98/134, 73.1%) and carcinoma cells (102/134, 76.1%). All 4 pancreatic cancer cell lines expressed FAP protein at different levels. Protein bands corresponding to the proteolytically active 170-kDa seprase dimer and its88-kDa seprase subunit were identif ied. Higher FAP expression in carcinoma cells was associated with tumor size (P < 0.001), fi brotic focus (P = 0.003), perineural invasion (P = 0.009) and worse clinical outcome (P = 0.0085). CONCLUSION: FAP is highly expressed in carcinoma cells and f ibroblasts in PDAC tissues, and its expression is associated with desmoplasia and worse prognosis.展开更多
AIM: To investigate the expression patterns of human differentiated embryo chondrocyte 1 (DEC1) in hepatocellular carcinoma (HCC) and corresponding adjacent non-tumor and the normal liver tissues, the association betw...AIM: To investigate the expression patterns of human differentiated embryo chondrocyte 1 (DEC1) in hepatocellular carcinoma (HCC) and corresponding adjacent non-tumor and the normal liver tissues, the association between DEC1 expression and histopathological variables and the role of DEC1 in hepatocarcinogenesis. METHODS: The expression of DEC1 was detected immunohistochemically in 176 paraffin-embedded sections from 63 patients with HCC and 50 subjects with normal liver tissues. RESULTS: DEC1 protein was persistently expressed in the cytoplasm of hepatocytes in normal liver and HCC tissues. Compared with adjacent non-tumor liver tissues, HCC tissues showed high nuclear expression of DEC1 protein. However, high DEC1 nuclear expression was more frequently detected in well-differentiated (83.3%) than in moderately (27.3%) and poorly differentiated HCC (16.7%). Low DEC1 expression was associated with poor histological differentiation and malignancy progression. A correlation was found between the nuclear expression of DEC1 protein and histological differentiation (r = 0.376, P = 0.024). CONCLUSION: DEC1 is expressed in the cytoplasm of hepatocytes and because nuclear DEC1 expression is decreased with decreasing differentiation status of HCC, nuclear DEC1 might be a marker of HCC differentiation.展开更多
BACKGROUND Gastric cancer(GC) is one of the most common malignancies in China with a high morbidity and mortality.AIM To determine whether interleukin(IL)-31, IL-32, and IL-33 can be used as biomarkers for the detecti...BACKGROUND Gastric cancer(GC) is one of the most common malignancies in China with a high morbidity and mortality.AIM To determine whether interleukin(IL)-31, IL-32, and IL-33 can be used as biomarkers for the detection of GC, via evaluating the correlations between their expression and clinicopathological parameters of GC patients.METHODS Tissue array(n = 180) gastric specimens were utilised. IL-31, IL-32, and IL-33 expression in GC and non-GC tissues was detected immunohistochemically. The correlations between IL-31, IL-32, and IL-33 expression in GC and severity of clinicopathological parameters were evaluated. Survival curves were plotted using the Kaplan-Meier method/Cox regression. Circulating IL-31, IL-32, and IL-33 were detected by ELISA.RESULTS We found that the expression levels of IL-31, IL-32, and IL-33 were all lower in GC than in adjacent non-GC gastric tissues(P < 0.05). IL-33 in peripheral blood of GC patients was significantly lower than that of healthy individuals(1.50 ± 1.11 vs 9.61 ± 8.00 ng/m L, P <0.05). Decreased IL-31, IL-32, and IL-33 in GC were observed in younger patients(< 60 years), and IL-32 and IL-33 were lower in female patients(P < 0.05). Higher IL-32 correlated with a longer survival in two GC subgroups: T4 invasion depth and TNM I-II stage. Univariate/multivariate analysis revealed that IL-32 was an independent prognostic factor for GC in the T4 stage subgroup. Circulating IL-33 was significantly lower in GC patients at TNM stage IV than in healthy people(P < 0.05).CONCLUSION Our findings may provide new insights into the roles of IL-31, IL-32, and IL-33 in the carcinogenesis of GC and demonstrate their relative usefulness as prognostic markers for GC. The underlying mechanism of IL-31, IL-32, and IL-33 actions in GC should be further explored.展开更多
Objective:To investigate the expression and significance of IL-38 protein in gastric carcinoma and normal tissues adjacent to carcinoma and to evaluate the relationship between the expressions and the clinical patholo...Objective:To investigate the expression and significance of IL-38 protein in gastric carcinoma and normal tissues adjacent to carcinoma and to evaluate the relationship between the expressions and the clinical pathologic factors.Methods:High throughput technology of tissue microarray and immunohistochemical SP method were applied to detect the expressions of IL-38 protein in 78 gastric carcinoma specimens that did not have a history of chemo-radiotherapy and 72 non-tumor gastric tissues.The followed-up cases were used for prognosis analysis.Results:The positive expression rate of IL-38 in gastric cancer tissues was 84.6%,and the expression rate in adjacent tissues was 45.8%.The difference between the two groups was statistically significant.IL-38 protein showed relationship with tumor TNM stage and lymph node metastasis.Kaplan-Meier survival analysis showed that the high expression of IL-38 was associated with poor prognosis of gastric cancer.Cox proportional hazard regression analysis showed that IL-38,tumor TNM clinical stage and lymph node metastasis were associated with prognosis,and IL-38 and lymph node metastasis could be used as independent prognostic factors.Conclusion:IL-38 is highly expressed in gastric cancer tissues,and the positive expression of IL-38 is associated with tumor TNM stage and lymph node metastasis.High expression of IL-38 is associated with poor prognosis in patients with gastric cancer,and IL-38 and lymph node metastasis can be used as independent prognostic factors.This experiment clarifies the relationship between IL-38 and clinicopathological parameters and prognosis of gastric cancer,and provides a new target for clinical diagnosis and treatment of gastric cancer.展开更多
To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G (1), was isolated from the roots of Vernonia cumingiana Benth. (Compositae).The structure ...To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G (1), was isolated from the roots of Vernonia cumingiana Benth. (Compositae).The structure of vernonioside G was elucidated using spectral methods, particularly two-dimensional nuclear magnetic resonance analysis. Together with the new compound, eight known compounds were also isolated and identified from the roots of V. cumingiana, among which, VE-1 (2) and 24-methylenelanost-9(11)-en-3β-ol acetate (3) were assigned NMR data for the first time and compound 3 was obtained as a natural product from a plant for the first time.展开更多
The tribe Delphineae,comprising two genera,Aconitum L.and Delphinium L.,is the richest source of bioactive diterpenoid alkaloids(DAs),while their highly similar chemical structures create a major challenge for derepli...The tribe Delphineae,comprising two genera,Aconitum L.and Delphinium L.,is the richest source of bioactive diterpenoid alkaloids(DAs),while their highly similar chemical structures create a major challenge for dereplication from complex mixture.To solve the inefficient discovery process of natural DAs,a novel dereplication strategy based on pattern recognition of specific heteronuclear multiple bond correlation(HMBC)signals has been established in this study.This strategy utilized the diagnostic signals in the HMBC spectra of C_(19)-DAs,which could indicate the structural categories.The newly designed software MatchNat allowed in silico recognition of C_(19)-DA types.The developed dereplication system was demonstrated to be a practical strategy to dereplicate a variety of natural products from two Delphineae plants,including Aconitum vilmorinianum Kom.and Delphinium pachycentrum Hemsl.Known compounds could be rapidly recognized from the mixture,and novel DAs were found with the aid of MatchNat.Consequently,targeted isolation led to the explosive discovery of a series of novel DAs.This dereplication system has the potential to be adopted for dereplication of any other class of natural products and has shown promising application as a complementary tool in the nuclear magnetic resonance analysis of traditional Chinese medicines.展开更多
基金Supported by Medical Science Research Project of Hebei Province in 2023(No.20231818).
文摘AIM:To access the agreement of intraocular pressure(IOP)values obtained from biomechanically corrected tonometer[Corvis ST(CST)],non-contact tonometer(NCT),and Goldmann applanation tonometer(GAT)in children with NCT measured-IOP(NCT-IOP)values of 22 mm Hg or more,and related factors.METHODS:A total of 51 eyes with NCT-IOP≥22 mm Hg in children aged 7 to 14y were examined and IOP was measured by CST,NCT,and GAT.Based on GAT measured IOP(GAT-IOP),ocular hypertension(OHT)group(≥22 mm Hg,24 eyes)and the non-OHT group(<22 mm Hg,27 eyes)were defined.We compared the agreement of the three measurements,i.e.,CST measured IOP(CST-IOP),GAT-IOP,and NCT-IOP,and further analyzed the correlation between the differences in tonometry readings,central corneal thickness(CCT),axial length(AL),optic disc rim volume,and age.RESULTS:Compared with the OHT group,thicker CCT,larger rim volume,and higher differences between NCTIOP and GAT-IOP,were found in the non-OHT group.The differences between CST-IOP and GAT-IOP were lower than the differences between NCT-IOP and GAT-IOP in both groups.The mean differences in CST-IOP and GAT-IOP were 1.26 mm Hg(95%limit of agreement ranged from 0.1 to 2.41 mm Hg,OHT group)and 1.20 mm Hg(95%limit of agreement ranged from-0.5 to 3.00 mm Hg,non-OHT group),and the mean differences in NCT and GAT were 3.90 mm Hg(95%limit of agreement ranged from-0.19 to 9.70 mm Hg,OHT group)and 6.00 mm Hg(95%limit of agreement ranged from 1.50 to 10.50 mm Hg,non-OHT group).The differences between CST-IOP and GAT-IOP were not related to CCT,age,and AL in both groups;while the differences between NCT-IOP and GAT-IOP were related to CCT in the OHT group(r=0.93,P<0.001)and to CCT and AL in the non-OHT group(r=0.66,P<0.001,r=-0.81,P<0.001).CONCLUSION:The accuracy of NCT in the diagnosis of pediatric OHT is low.The agreement of CST-IOP and GATIOP was significantly higher in children with and without OHT than in those with NCT-IOP and GAT-IOP.Therefore,CST can be used as a good alternative for IOP measurement in children.The impacts of CCT and AL on NCT measurement need to be fully considered when managing childhood IOP.
基金Supported by The National Key Project of Scientific and Technical Supporting Programs of China, No. 2006BAI02A14National Natural Science Foundation of China, No. 30770996 and No. 81172310
文摘AIM: To examine fibroblast activation protein (FAP) expression in pancreatic ductal adenocarcinoma (PDAC) and to analyze its relationship with the clinicopathology of PDAC. METHODS: FAP expression was examined in 134 PDAC specimens by immunohistochemistry, and in four pancreatic cancer cell lines (SW1990, Miapaca-2, AsPC-1 and BxPC-3) by Western blotting assay. We also analyzed the association between FAP expression in PDAC cells and the clinicopathology of PDAC patients. RESULTS: The results showed that the FAP was expressed in both stromal fibroblast cells (98/134, 73.1%) and carcinoma cells (102/134, 76.1%). All 4 pancreatic cancer cell lines expressed FAP protein at different levels. Protein bands corresponding to the proteolytically active 170-kDa seprase dimer and its88-kDa seprase subunit were identif ied. Higher FAP expression in carcinoma cells was associated with tumor size (P < 0.001), fi brotic focus (P = 0.003), perineural invasion (P = 0.009) and worse clinical outcome (P = 0.0085). CONCLUSION: FAP is highly expressed in carcinoma cells and f ibroblasts in PDAC tissues, and its expression is associated with desmoplasia and worse prognosis.
基金Supported by The National Natural Science Foundation ofChina, No. 81000869the "Spring City Scholars" ConstructionProject of Jinan City (Q2-06)+1 种基金the Key Projects of Science andTechnology of Jinan City, No. 200807027the Youth Sci-ence and Technology Star Project of Jinan City, No. 20080210
文摘AIM: To investigate the expression patterns of human differentiated embryo chondrocyte 1 (DEC1) in hepatocellular carcinoma (HCC) and corresponding adjacent non-tumor and the normal liver tissues, the association between DEC1 expression and histopathological variables and the role of DEC1 in hepatocarcinogenesis. METHODS: The expression of DEC1 was detected immunohistochemically in 176 paraffin-embedded sections from 63 patients with HCC and 50 subjects with normal liver tissues. RESULTS: DEC1 protein was persistently expressed in the cytoplasm of hepatocytes in normal liver and HCC tissues. Compared with adjacent non-tumor liver tissues, HCC tissues showed high nuclear expression of DEC1 protein. However, high DEC1 nuclear expression was more frequently detected in well-differentiated (83.3%) than in moderately (27.3%) and poorly differentiated HCC (16.7%). Low DEC1 expression was associated with poor histological differentiation and malignancy progression. A correlation was found between the nuclear expression of DEC1 protein and histological differentiation (r = 0.376, P = 0.024). CONCLUSION: DEC1 is expressed in the cytoplasm of hepatocytes and because nuclear DEC1 expression is decreased with decreasing differentiation status of HCC, nuclear DEC1 might be a marker of HCC differentiation.
基金Supported by the National Natural Science Foundation of China,No. 81502030。
文摘BACKGROUND Gastric cancer(GC) is one of the most common malignancies in China with a high morbidity and mortality.AIM To determine whether interleukin(IL)-31, IL-32, and IL-33 can be used as biomarkers for the detection of GC, via evaluating the correlations between their expression and clinicopathological parameters of GC patients.METHODS Tissue array(n = 180) gastric specimens were utilised. IL-31, IL-32, and IL-33 expression in GC and non-GC tissues was detected immunohistochemically. The correlations between IL-31, IL-32, and IL-33 expression in GC and severity of clinicopathological parameters were evaluated. Survival curves were plotted using the Kaplan-Meier method/Cox regression. Circulating IL-31, IL-32, and IL-33 were detected by ELISA.RESULTS We found that the expression levels of IL-31, IL-32, and IL-33 were all lower in GC than in adjacent non-GC gastric tissues(P < 0.05). IL-33 in peripheral blood of GC patients was significantly lower than that of healthy individuals(1.50 ± 1.11 vs 9.61 ± 8.00 ng/m L, P <0.05). Decreased IL-31, IL-32, and IL-33 in GC were observed in younger patients(< 60 years), and IL-32 and IL-33 were lower in female patients(P < 0.05). Higher IL-32 correlated with a longer survival in two GC subgroups: T4 invasion depth and TNM I-II stage. Univariate/multivariate analysis revealed that IL-32 was an independent prognostic factor for GC in the T4 stage subgroup. Circulating IL-33 was significantly lower in GC patients at TNM stage IV than in healthy people(P < 0.05).CONCLUSION Our findings may provide new insights into the roles of IL-31, IL-32, and IL-33 in the carcinogenesis of GC and demonstrate their relative usefulness as prognostic markers for GC. The underlying mechanism of IL-31, IL-32, and IL-33 actions in GC should be further explored.
基金This work was supported by National Natural Science Foundation of China （Grant No. 10274077, 20103007 and 29703007）. The authors would like to thank Wei Chu, Yong-qiang Xu and Guo-sheng Cheng for their kind help.
文摘气体的 CH <SUB>2</SUB 的红外线的吸收系列 > 在 120012000 厘米 <SUP>1</SUP> 的区域的 Cl <SUB>2</SUB> 用 Bruker 被测量与一个 multipass 房间一起的 IFS 120HR Fourier 变换分光计。陪音和 combinational 的 47 个震动的层次光谱拉长的 CH 的线(v <SUB>1</SUB>, v <SUB>6</SUB>), 弯曲(v <SUB>2</SUB>), 并且摇(v <SUB>8</SUB>) 模式被分析并且分配。利用正常模式模型并且考虑在拉长的 CH 之中联合，把颤动弄弯并且摇，泛音频率 <SUB 的值> i </SUB>,非谐的经常的 x <SUB > ij </SUB>,和费密的系数和 v <SUB>1</SUB>, v <SUB>6</SUB>, v <SUB>2</SUB>和 v <SUB>8</SUB>模式的 Darling-Dennison 回声也被决定从试验性光谱有非线性的最少平方的适合的数据。这些光谱常数复制了试验性的层次很好。这些结果显示出在拉长并且摇颤动的 CH 之间的那费密回声(254.63 厘米 <SUP>1</SUP>) 是的 k <SUB>188</SUB>= 比那强壮在拉长并且弯曲颤动的 CH 之间(k <SUB>122</SUB>= 54.87 厘米 <SUP>1</SUP>) ；并且那 Darling-Dennison 在拉长并且弯曲颤动的 CH 之间的回声(k <SUB>1166</SUB>= 215.28 厘米 <SUP>1</SUP>) 在 CH 把颤动弄弯并且摇之间也是比那强壮得多的(k <SUB>2288</SUB>= 5.72 厘米 <SUP>1</SUP>) 。
基金National Natural Science Foundation of China(81502030).
文摘Objective:To investigate the expression and significance of IL-38 protein in gastric carcinoma and normal tissues adjacent to carcinoma and to evaluate the relationship between the expressions and the clinical pathologic factors.Methods:High throughput technology of tissue microarray and immunohistochemical SP method were applied to detect the expressions of IL-38 protein in 78 gastric carcinoma specimens that did not have a history of chemo-radiotherapy and 72 non-tumor gastric tissues.The followed-up cases were used for prognosis analysis.Results:The positive expression rate of IL-38 in gastric cancer tissues was 84.6%,and the expression rate in adjacent tissues was 45.8%.The difference between the two groups was statistically significant.IL-38 protein showed relationship with tumor TNM stage and lymph node metastasis.Kaplan-Meier survival analysis showed that the high expression of IL-38 was associated with poor prognosis of gastric cancer.Cox proportional hazard regression analysis showed that IL-38,tumor TNM clinical stage and lymph node metastasis were associated with prognosis,and IL-38 and lymph node metastasis could be used as independent prognostic factors.Conclusion:IL-38 is highly expressed in gastric cancer tissues,and the positive expression of IL-38 is associated with tumor TNM stage and lymph node metastasis.High expression of IL-38 is associated with poor prognosis in patients with gastric cancer,and IL-38 and lymph node metastasis can be used as independent prognostic factors.This experiment clarifies the relationship between IL-38 and clinicopathological parameters and prognosis of gastric cancer,and provides a new target for clinical diagnosis and treatment of gastric cancer.
文摘To search for new and bioactive constituents from traditional Chinese medicines, a new steroidal saponin, named vernonioside G (1), was isolated from the roots of Vernonia cumingiana Benth. (Compositae).The structure of vernonioside G was elucidated using spectral methods, particularly two-dimensional nuclear magnetic resonance analysis. Together with the new compound, eight known compounds were also isolated and identified from the roots of V. cumingiana, among which, VE-1 (2) and 24-methylenelanost-9(11)-en-3β-ol acetate (3) were assigned NMR data for the first time and compound 3 was obtained as a natural product from a plant for the first time.
基金funded by the Science and Technology Development Fund,Macao SAR(File Nos.0023/2019/AKP,0077/2019/A2,0002/2019/APD,and 0040/2021/AGJ)the National Natural Science Foundation of China(No.31860095)。
文摘The tribe Delphineae,comprising two genera,Aconitum L.and Delphinium L.,is the richest source of bioactive diterpenoid alkaloids(DAs),while their highly similar chemical structures create a major challenge for dereplication from complex mixture.To solve the inefficient discovery process of natural DAs,a novel dereplication strategy based on pattern recognition of specific heteronuclear multiple bond correlation(HMBC)signals has been established in this study.This strategy utilized the diagnostic signals in the HMBC spectra of C_(19)-DAs,which could indicate the structural categories.The newly designed software MatchNat allowed in silico recognition of C_(19)-DA types.The developed dereplication system was demonstrated to be a practical strategy to dereplicate a variety of natural products from two Delphineae plants,including Aconitum vilmorinianum Kom.and Delphinium pachycentrum Hemsl.Known compounds could be rapidly recognized from the mixture,and novel DAs were found with the aid of MatchNat.Consequently,targeted isolation led to the explosive discovery of a series of novel DAs.This dereplication system has the potential to be adopted for dereplication of any other class of natural products and has shown promising application as a complementary tool in the nuclear magnetic resonance analysis of traditional Chinese medicines.
