Antitumor and synergistic effect of Chinese medicine “Bushen huayu jiedu recipe” and chemotherapy on transplanted animal hepatocarcinoma

AIM: To investigate the antitumor and synergistic effect of Chinese medicine 'Bushen huayu jiedu recipe' (recipe for invigorating the kidney, removing blood stasis and toxic substances) and chemotherapy on mice hepatocarcinoma.METHODS: Bushen huayu jiedu recipe (BSHYJDR)consisting of Chinese Cassia Bark, Psoralea, Zedoary,Rhubarb, etc. is equal to 1.5 g/mL liquid of originated herbs after being decocted, filtered, and concentrated. Kunming mice, weighing 18-22 g, were injected with 0.2 mL ascitic hepatocarcinoma H22 containing 1×107 cells/mL into armpit of the right forelimb of mice. After 24 h, the mice were weighed and randomly divided into tumor-bearing model control group, cisplatin (DDP) group, BSHYJDR high dosage group, low dosage BSHYJDR group, DDP combined with high and low dosage BSHYJDR group, 10 mice in each group. DDP group received injection intraperitoneally (ip) at the dosage of 1 mg/kg (equal to 1/10 LD50), once a day for 4 d. High and low dosage BSHYJDR groups received intragastric BSHYJDR at the dosages of 26.6 and 13.3 g/kg (20 and 10 times each of clinical adult dosage)respectively, while tumor-bearing model group received the equal volume of distilled water once a day for 10 d. On the 11th d, the mice were weighed and killed, then the tumor was dissected and weighed, the repression rate (RR) was calculated according to the mean weight of tumor (MWT).RESULTS: Compared to the model group (MWT: 1.30±0.73),DDP group (MWT: 0.41+0.09, RR: 68.46%) had a significant difference in the inhibition of hepatocarcinoma H22 (P＜0.01).High dosage BSHYJDR group (MWT: 0.69+0.29, RR:46.92%) also had a significant difference in inhibition (P＜0.05), while no difference was found in low dosage BSHYJDR group (MWT: 0.85+0.34, RR: 34.62%) (P＞0.05).When DDP was combined with high dosage BSHYJDR (MWT: 0.29±0.17, RR: 77.69%) and low dosage BSHYJDR (MWT: 0.38±0.21, RR: 70.77%) respectively, we could see improvement of the inhibition effect of DDP on transplanted hepatocarcinoma H22. DDP combined with high dosage BSHYJDR had a significant difference (P＜0.001) compared to DDP, while DDP combined with low dosage BSHYJDR only had a little improvement that is not remarkable.CONCLUSION: Chinese medicine BSHYJDR in combination with chemotherapy can inhibit transplanted hepatocarcinoma in mice.

细胞免疫治疗联合化疗对实体肿瘤疗效的荟萃分析（英文）

目的:分析和研究DC-CIK细胞免疫治疗是否能够增强化疗在实体肿瘤中的疗效。创新点:首次荟萃分析了关于DC-CIK细胞免疫治疗联合化疗治疗肿瘤疗效的随机对照试验,并从多个角度对疗效进行综合评价。方法:通过文献检索及筛选,收集关于化疗联合DC-CIK细胞免疫治疗的随机对照试验(图1),并从中提取相关数据进行荟萃分析。总生存率(OS)、无病生存率(DFS)、客观缓解率(ORR)、疾病控制率(DFS)和治疗后T淋巴细胞亚型水平是本研究中的主要疗效指标,比值比(OR)(图3、4和5)以及平均差(MD)(图6)用来评价各个指标的综合效应。结论:共有12个随机对照试验被纳入我们的研究中(表1和2)。相对于单独应用化疗,DC-CIK细胞治疗联合化疗的综合疗法能在1年生存率、2年生存率、3年生存率、1年无病生存率、3年无病生存率、客观缓解率以及疾病控制率等指标上有明显优势,且接受综合疗法患者的CD3+T淋巴细胞和CD4+T淋巴细胞水平明显更高。综上所述,DC-CIK细胞免疫治疗能够增强化疗对实体肿瘤的疗效。