Moringa oleifera leaves (MO L) attract extensive attention in agriculture,pharmaceutical and food industry because of its rich biological activity.In this study,ChCl/LA as one of the deep eutectic solvents (DESs) was ...Moringa oleifera leaves (MO L) attract extensive attention in agriculture,pharmaceutical and food industry because of its rich biological activity.In this study,ChCl/LA as one of the deep eutectic solvents (DESs) was selected to extract flavonoids from Moringa oleifera leaves (FMO L).The extraction rate of FMO L reached 54.8 mg/g.After separation of FMO L,its biological activities were systematically studied.The IC50 values of FMO L for DPPH radical,ABTS radical scavenging activity,FRAP and ORAC were 27.3 μg/mL,41.2 μg/mL,18.7 mg TE/g and 6008.4 mg TE/g,respectively,indicating FMO L had excellent antioxidant activity.FMO L not only had better antibacterial activity than the positive controls,but also had inhibitory activity to tumor cells.The IC50 values of FMO L on B16 cells,HCT116 cells and Hep G-2 cells were 530.7,328.0,and 533.7 μg/mL,respectively.Nine flavonoid compounds were identified by UPLC-Q-Orbitrap MS/MS,and four of them were confirmed from MO L for the first time.Therefore,FMO L extracted by ChCl/LA had rich bioactive components and is expected to be used in food and pharmaceutical industries.展开更多
基金supported by Guangdong Basic and Applied Basic Research Foundation(No.2020A1515110427,2022A1515010009)the Youth Innovation Project of Guangdong Province University(No.2020KQNCX088)+5 种基金the National Natural Science Foundation of China(No.21802019)the Characteristics Innovation Project of Guangdong Province University(No.2019KTSCX185)COVID-19 Epidemic Prevention and Control Project(No.2020FKZX02)Foundation from Department of Education of Guangdong Province(2017KSYS010)Innovation and Entrepreneurship Training Project of Wuyi University(No.2020CX35)Youth Research Fund Team Project of Wuyi University(No.2019td05).
文摘Moringa oleifera leaves (MO L) attract extensive attention in agriculture,pharmaceutical and food industry because of its rich biological activity.In this study,ChCl/LA as one of the deep eutectic solvents (DESs) was selected to extract flavonoids from Moringa oleifera leaves (FMO L).The extraction rate of FMO L reached 54.8 mg/g.After separation of FMO L,its biological activities were systematically studied.The IC50 values of FMO L for DPPH radical,ABTS radical scavenging activity,FRAP and ORAC were 27.3 μg/mL,41.2 μg/mL,18.7 mg TE/g and 6008.4 mg TE/g,respectively,indicating FMO L had excellent antioxidant activity.FMO L not only had better antibacterial activity than the positive controls,but also had inhibitory activity to tumor cells.The IC50 values of FMO L on B16 cells,HCT116 cells and Hep G-2 cells were 530.7,328.0,and 533.7 μg/mL,respectively.Nine flavonoid compounds were identified by UPLC-Q-Orbitrap MS/MS,and four of them were confirmed from MO L for the first time.Therefore,FMO L extracted by ChCl/LA had rich bioactive components and is expected to be used in food and pharmaceutical industries.
