Correction to:Light:Science&Applications(2018)7,17153;https://doi.org/10.1038/lsa.2017.153;Article published online,23 February 2018.In the version of this article originally published,in the“Materials and method...Correction to:Light:Science&Applications(2018)7,17153;https://doi.org/10.1038/lsa.2017.153;Article published online,23 February 2018.In the version of this article originally published,in the“Materials and methods”section under subheading“Reagents”,there are errors about the information of reagents.And the original and corrected versions are shown below.展开更多
Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena.Here,we introduce a skull optical clearing window for imaging cortical structures at synaptic re...Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena.Here,we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution.Combined with two-photon microscopy,this technique allowed us to repeatedly image neurons,microglia and microvasculature of mice.We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation.Given its easy handling and safety,this method holds great promise for application in neuroscience research.展开更多
文摘Correction to:Light:Science&Applications(2018)7,17153;https://doi.org/10.1038/lsa.2017.153;Article published online,23 February 2018.In the version of this article originally published,in the“Materials and methods”section under subheading“Reagents”,there are errors about the information of reagents.And the original and corrected versions are shown below.
基金supported by the National Natural Science Foundation of China(Grants Nos.91232710,31571002)the Science Fund for Creative Research Groups(Grant No.61721092)the Director Fund of WNLO.We are thankful to ZH Zhang for providing the Cx3cr1^(EGFP/+)mice.
文摘Imaging cells and microvasculature in the living brain is crucial to understanding an array of neurobiological phenomena.Here,we introduce a skull optical clearing window for imaging cortical structures at synaptic resolution.Combined with two-photon microscopy,this technique allowed us to repeatedly image neurons,microglia and microvasculature of mice.We applied it to study the plasticity of dendritic spines in critical periods and to visualize dendrites and microglia after laser ablation.Given its easy handling and safety,this method holds great promise for application in neuroscience research.
