Copper azide with high density was successfully synthesized by in-situ reaction of nanoporous copper(NPC)precursor with HN_(3) gaseous.NPC with pore size of about 529 nm has been prepared by electroless plating using ...Copper azide with high density was successfully synthesized by in-situ reaction of nanoporous copper(NPC)precursor with HN_(3) gaseous.NPC with pore size of about 529 nm has been prepared by electroless plating using polystyrene(PS)as templates.The copper shells thickness of NPC was controlled by adjusting the PS loading amount.The effects of copper shell on the morphology,structure and density of copper azide were investigated.The conversion increased from 87.12%to 95.31%when copper shell thickness decrease from 100 to 50 nm.Meanwhile,the density of copper azide prepared by 529 nm NPC for 24 h was up to 2.38 g/cm^(3).The hollow structure of this NPC was filled by swelling of copper azide which guaranteed enough filling volume for keeping the same shape as well as improving the charge density.Moreover,HNS-IV explosive was successfully initiated by copper azide with minimum charge thickness of 0.55 mm,showing that copper azide prepared has excellent initiation performance,which has more advantages in the application of miniaturized explosive systems.展开更多
Seven photoaffinity-based and sixteen biotin-based berberine(BBR)probes were constructed and screened for their effects on c-Jun N-terminal protein kinases(JNK)phosphorylation(p-JNK)suppression at the cellular level.T...Seven photoaffinity-based and sixteen biotin-based berberine(BBR)probes were constructed and screened for their effects on c-Jun N-terminal protein kinases(JNK)phosphorylation(p-JNK)suppression at the cellular level.Taking active-photoaffinity probe 7c as a chemical tool,we first identified mitogen-activated protein kinase 7(MAP2K7),an upstream protein on the JNK/stress activated protein kinase(SAPK)pathway,as a direct proteomic target of BBR using activity-based protein profiling(ABPP)and other chemical proteomic techniques.Furthermore,BBR’s inhibitory effect on p-JNK was significantly attenuated in both the MAP2K7-knockdown and models,indicating a MAP2K7-dependent inhibition on the JNK signaling pathway.For the first time,we demonstrate the unique mechanism of BBR that directly targets MAP2K7 to inhibit p-JNK rather than JNK activity with the advantages of multiple activities and a good safety profile.展开更多
基金the financial support provided by the National Natural Science Foundation of China(No.11872013)。
文摘Copper azide with high density was successfully synthesized by in-situ reaction of nanoporous copper(NPC)precursor with HN_(3) gaseous.NPC with pore size of about 529 nm has been prepared by electroless plating using polystyrene(PS)as templates.The copper shells thickness of NPC was controlled by adjusting the PS loading amount.The effects of copper shell on the morphology,structure and density of copper azide were investigated.The conversion increased from 87.12%to 95.31%when copper shell thickness decrease from 100 to 50 nm.Meanwhile,the density of copper azide prepared by 529 nm NPC for 24 h was up to 2.38 g/cm^(3).The hollow structure of this NPC was filled by swelling of copper azide which guaranteed enough filling volume for keeping the same shape as well as improving the charge density.Moreover,HNS-IV explosive was successfully initiated by copper azide with minimum charge thickness of 0.55 mm,showing that copper azide prepared has excellent initiation performance,which has more advantages in the application of miniaturized explosive systems.
基金ACKNOWLEDGMENTS This work was supported by the National Natural Science Foundation of China （No.20073004, No.20473012, No.20673010, and No.20640420450）, the 111 Project B07012, and the China Postdoctoral Science Foundation （No.20070410466）. The Trans-Century Training Program Foundation for the Talents by the Ministry of Education of China was also gratefully acknowledged.
基金supported by the CAMS Innovation Fund for Medical Sciences(nos.2020-I2M-2-010 and 2016-I2M-1-011)the Drug Innovation Major Project(no.2018ZX09711-001)the National Natural Science Foundation of China(no.81974494).
文摘Seven photoaffinity-based and sixteen biotin-based berberine(BBR)probes were constructed and screened for their effects on c-Jun N-terminal protein kinases(JNK)phosphorylation(p-JNK)suppression at the cellular level.Taking active-photoaffinity probe 7c as a chemical tool,we first identified mitogen-activated protein kinase 7(MAP2K7),an upstream protein on the JNK/stress activated protein kinase(SAPK)pathway,as a direct proteomic target of BBR using activity-based protein profiling(ABPP)and other chemical proteomic techniques.Furthermore,BBR’s inhibitory effect on p-JNK was significantly attenuated in both the MAP2K7-knockdown and models,indicating a MAP2K7-dependent inhibition on the JNK signaling pathway.For the first time,we demonstrate the unique mechanism of BBR that directly targets MAP2K7 to inhibit p-JNK rather than JNK activity with the advantages of multiple activities and a good safety profile.
