Comparison of therapeutic effectiveness of combined interventional therapy for 1126 cases of primary liver cancer

瞄准：为肝细胞癌(HCC ) 验证联合 interventional 治疗的效果。方法：接待了的 1126 个 HCC 病人的临床的数据为 transcatheter 联合了 interventional 治疗动脉的 chemoembolization (不作声)在肝切除术前后，不作声并且无线电频率脱离( RFA )在以后，中国药治疗和简历治疗不作声或 transcatheter 动脉的注入(傣族)，根据他们的肝函数， alpha-fetoprotein ，图象数据， color-ultrasonography 发现和幸存率的结果被考察。结果：874 个病人的一个总数被跟随在上面为 2 ～ 63 瞬间的一个时期。年幸存评估的全面的 1- ， 3- 和 5- 分别地是 67.8% ， 28.7% 和 18.8% 。接待了的病人的幸存率不作声的 1-3- 和 5- 年是 74.7% ， 41.4% ， 36.9% 在肝切除术前并且 78.9% ， 40.4% ， 37.5% 在肝切除术以后。有效的率(PR + NC ) 在以后不作声并且 RFA 是 93.4% ， 1- 和 3- 年幸存率是 74.5% 和 36.8% 在以后不作声并且 RFA。PR + NC 在以后的有效的率不作声是 83.2% 。年幸存评估的 1- ， 3- 和 5- 是 69.3% ， 21.7% ， 8.4% 在以后不作声。PR + NC 的有效的率在傣族是 27.5% 以后， 1- 和 2- 年幸存率在傣族以后是 11.6% 和 0% 。肝功能， color-ultrasonography 发现和 alpha-fetoprotein 在以后不作声 + RFA，不作声，傣族被比较。在每个索引在之间没有有效差量不作声并且 RFA 或象在肝功能在之间一样不作声不作声并且 RFA 或在之间不作声并且傣族。结论：治疗学的有效性在肝切除术前后不作声，是很重要的效果不作声并且 RFA 比的好不作声，并且傣族的效果是最小的。

Effectiveness of experiential teaching method on the development of nursing students’skill competence:a systematic review and meta-analysis

Objective:Skill competence is essential for nursing students and experiential teaching method has become increasingly popular in China’s nursing education.To systematically evaluate the effects of experiential teaching method on the development of nursing students’skill competence,and to provide evidence for nursing education.Methods:The China National Knowledge Infrastructure,VIP,Wanfang Data,PubMed,and Web of Science databases were searched from their inception until November 28,2019.We screened the studies according to inclusion and exclusion criteria,extracted the data,and assessed the quality.Then,a meta-analysis was conducted.Results:Totally 15 studies were included,and 1,633 nursing students were analyzed by Review Manager5.3 and Stata15.1 software.The results show that the experiential teaching method can significantly improve nursing students’nursing skills such as training(SMD=1.05,95%CI:0.95–1.16,P=0.000),learning interest(ES=0.93,95%CI:0.79–1.06),ability to analyze and solve problems(ES=0.75,95%CI:0.55–0.94),ability to judge and innovative thinking(ES=1.01,95%CI:0.77–1.25),abilities of understanding and perception(ES=0.59,95%CI:0.34–0.83),and teaching satisfaction(ES=1.26,95%CI:1.13–1.4).Conclusions:The results suggest that the experiential teaching method is more effective for the nursing students’skill competence than traditional teaching in China.However,due to the heterogeneity and bias risk,a large sample and high-quality studies are needed in future to confirm its effectiveness.

Effect of resveratrol on pancreatic oxygen free radicals in rats with severe acute pancreatitis

瞄准：在试验性的严重尖锐胰腺炎(树液) 上作为自由基 scavenger 调查 resveratrol (RESV ) 的治疗学的效果。方法：72 只男 Sprague-Dawley 老鼠随机被划分成假冒的操作组，树液组，和对待 resveratrol 的组。胰腺炎被 0.1 mL/kg 的管内管理导致 4% 牛磺胆酸钠。RESV 在 20 mg/kg 体重的剂量静脉内地被给。所有动物在 3 点被打死， 6，在模型的正式就职以后的 12 h。浆液淀粉酶，胰腺的超级氧化物歧化酶(草皮) ， malondialdehyde (MDA ) ，和 myeloperoxidase (军邮局) 是坚定的。胰的病理学的变化在光显微镜下面被观察。结果：浆液淀粉酶，胰腺的军邮局和病理学的损坏的分数在胰腺炎的正式就职以后增加了，早(3， 6 h ) 树液样品被减少的胰腺的草皮描绘并且增加了胰腺的 MDA。Resveratrol 在氧自由基在树液的早阶段引起的房间膜每氧化对类脂化合物展出了保护的效果。氧化还原作用状态缺陷的这变细减少了细胞的氧化损坏，由更低的浆液淀粉酶思考了不太严重的胰腺的损害，正常胰腺的 MDA 层次，以及在胰的减少的嗜中性的渗入。结论：RESV 可以由降低胰腺的氧化自由基并且减少 neutrophils 的胰腺的织物渗入在树液上施加它的治疗学的效果。

PAd-shRNA-PTN reduces pleiotrophin of pancreatic cancer cells and inhibits neurite outgrowth of DRG

AIM:To investigate the silencing effects of pAdshRNA-pleiotrophin(PTN) on PTN in pancreatic cancer cells,and to observe the inhibition of pAd-shRNA-PTN on neurite outgrowth from dorsal root ganglion(DRG) neurons in vitro.METHODS:PAd-shRNA-PTN was used to infect pancreatic cancer BxPC-3 cells;assays were conducted for knockdown of the PTN gene on the 0th,1st,3rd,5th,7th and 9th d after infection using immunocytochemistry,real-time quantitative polymerase chain reaction(PCR),and Western blotting analysis.The morphologic changes of cultured DRG neurons were observed by mono-culture of DRG neurons and co-culture with BXPC-3 cells in vitro.RESULTS:The real-time quantitative PCR showed that the inhibition rates of PTN mRNA expression in the BxPC-3 cells were 20%,80%,50% and 25% on the 1st,3rd,5th and 7th d after infection.Immunocytochemistry and Western blotting analysis also revealed the same tendency.In contrast to the control,the DRG neurons co-cultured with the infected BxPC-3 cells shrunk;the number and length of neurites were significantly decreased.CONCLUSION:Efficient and specific knockdown of PTN in pancreatic cancer cells and the reduction in PTN expression resulted in the inhibition of neurite outgrowth from DRG neurons.

Effect of spleen on immune function of rats with liver cancer complicated by liver cirrhosis

OBJECTIVE: To establish a rat model of liver cancer complicated by liver cirrhosis and explore the effects of the spleen on immune function in this model. METHODS: Liver cirrhosis was inflicted in rats by percutaneous injection of 40% CCl_4 on the back. Walker-256 tumor cells were inoculated in the cirrhotic liver and splenectomy was performed. Two weeks later, the growth and metastasis of tumor were observed and the amount of ascites and the activity of NK cells and CD25 cells were investigated. RESULTS: The amount of ascites and tumor volume were significantly higher in splenectomy group than in controls (P<0.O1). Two weeks after inoculation, the activity of NK cells in both groups was decreased as compared with that before the inoculation (P<0.01). There was no significant difference between the two groups before and after the inoculation (P>0.05). The number of CD25 in both groups was higher than that before the inoculation (P<0.01). However, there was no significant difference between the two groups before and after the inoculation (P>0.05). CONCLUSIONS: Splenectomy in early stage of tumor inoculation can stimulate the tumor growth and metastasis. The activity of NK cells and the number of CD25 are inhibited by tumor itself, not by splenectomy.